LOCUS       plasmid                 7249 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2156..3016)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(803..1182)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(599..802)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   complement(3933..4073)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(4578..4689)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3017..3121)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(1397..1985)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(3403..3858)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(525..551)
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4204..4444)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3877..3928
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4751..4773)
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    3353..3402
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3216..3251
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4781..4803)
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(1297..1324)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   complement(1241..1268)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 ggcttgtgag gagacagcag gatcagcctc tcgtgctcgg acacgttgca tctgccgaag
       61 tagttggtgt ggggcttgcc cttgtcgttc acttgttcca gcttgtacac gtacagaggc
      121 tggcaacact ggctgcaggc aaagccgcta gaggtcagtc tctcgttgcc gtccttcacg
      181 gggatcagct ttctctggtc gatgttctta ggagcgggct gagcaactct gctcatcagg
      241 cccacggggt ctttctggaa ctcttcccag tacacgtgca ggatggcttt cttcatctcg
      301 gtgtagtcgg tcagcagctt gttcaggttg gccctgctgg tgttgctgat aggctgaggg
      361 atgttctcgg gcttctttct caggttttcc agccgctctc tcaggtcagg ggtcatcact
      421 ctgacgagca gggttttcat ggggccgcgc ttgccggcct tctttgtgtt gctgtccttg
      481 accagtctcc ttctgatctt gttgattctc ttgatctctt gcatgtccaa cttgaccctc
      541 ttggcagcag gggatccgga catggtggca gcgctctaga accggtagtt agccagagag
      601 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      661 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      721 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      781 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      841 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      901 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      961 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     1021 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     1081 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     1141 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcctc gagtctagag
     1201 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     1261 tgaggccggg cgaccaaagg tcgcccgacg cccgggcggc ctcagtgagc gagcgagcgc
     1321 gcagctgcct gcaggacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1381 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1441 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1501 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1561 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1621 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1681 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1741 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1801 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1861 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1921 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1981 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2041 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2101 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2161 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2221 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2281 ctgcaatgat accgcggctt ccacgctcac cggctccaga tttatcagca ataaaccagc
     2341 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2401 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2461 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2521 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2581 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2641 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2701 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2761 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2821 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2881 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2941 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3001 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3061 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3121 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3181 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     3241 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3301 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     3361 actatgcggc atcagagcag attgtactga gagtgcacca taaaattgta aacgttaata
     3421 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caatagaccg
     3481 aaatcggcaa aatcccttat aaatcaaaag aatagcccga gatagagttg agtgttgttc
     3541 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3601 ccgtctatca gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt
     3661 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3721 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3781 aggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3841 cgccgctaca gggcgcgtac tatggttgct ttgacgtatg cggtgtgaaa taccgcacag
     3901 atgcgtaagg agaaaatacc gcatcaggcg cccctgcagg cagctgcgcg ctcgctcgct
     3961 cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
     4021 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg
     4081 caaacggcat gtcttcgaga agaccctttg atgctccctc cgaagaggga gctttaccca
     4141 ctacgacata gtcgctggtg atggctctca aagtaatcag ataaaagcgc cagtcggtgt
     4201 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     4261 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     4321 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
     4381 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     4441 cctcgcggcc agactaggcg gccgtaccaa aaaagggtac ctccccagca tgcctgctat
     4501 tctcttccca atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac
     4561 acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg
     4621 caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag
     4681 aaggcacagt cgaggctgat cagcgagctc taggaattct taggcgtagt caggtacgtc
     4741 gtaaggatag gcgtaatcgg gtacgtcgta tggataagcg tagtcaggca catcgtatgg
     4801 gtaactgccg accttccgct tcttctttgg caatgcattc acggcgggct tccagacttc
     4861 tttcagcttt tttctgtaga agctctgcca ggtttccaca aaggccctct tgtcggtgtt
     4921 gccggtggtc ttgttggtct ggtacttctt gtactcttgg cttctcagga acagccagct
     4981 tctggcaatg ttcagggcag cctgttcatc ggcgtgtgtc tcgaagccgc agttcaggca
     5041 cacgaacttc tcttgcacag gtctgtggct aaacctcttc ttcagcaggc tcagagcctc
     5101 gcctgatctg ccctttgtcc agctgctgat gtcgttgttc acgctctctt cgctcagtct
     5161 gtccagttcc acgctcagat ccttcacgac gttctgcctc ttgtacctgt tgtagtaggt
     5221 gatctggccc tcaaccttca gctctttgcc gttgatggtg gtcatccagc cggtggcggt
     5281 tttcttgagc ttttccagca ctctgtcgta gtcggcgctg gtgatggtga agccgcaatt
     5341 gctgcaggtg cgggaggtat actgggccag tgttttggac aggtaggtct tgctgggcag
     5401 tccctcgtag gccagtttag ctgtgagcca atcttccatt ctggtgtact gcctctcggc
     5461 cataaaggtt ctcttgccct gccggccgaa gcctctgctc agattctcga agatcagcat
     5521 ggcgtcctgt gtcacggcgt agtacagcag atctctggcg gtgtttcgga ccatgtcgtc
     5581 ggccagattc ttggccttgc tggcgtactt cctagagtat ccgccggctc ttctctgttc
     5641 cacctctttg gcggcctgga ttgtcctctg cttctctttg tagctctcgc cgattctcag
     5701 gatgtgtgta gggttgccca gggagtcctt gaatctgctc agtgggcatc cttcagggtc
     5761 tgtcagggcg atcacagcag ggatattctc gccccggtcg atgccgatca ggttcatggg
     5821 cttgatgttg ctgctgtcca gcacctctct tctctcgaag gtcagggcca caaacagagc
     5881 aggctcgtcc tgtctggttc tcctgttgta cagggttttc tcgatcactc tgccattggc
     5941 caggcgcaga gagccggttt ccaggctcag cagatcgttc cagatgaact ctctgccctg
     6001 tctcttgccg aaggccagag gcaggataat caggttgggg tcgtcgaagt tgaagttcac
     6061 ttccatgggc acgatctcgc cggacttttt gttgatcacg gtgtagaatc tgttagcctc
     6121 gaaggcctcg ggtttgatct tcttgaaccg cagcttgccg cctttgaagt aattgatgat
     6181 caggtacagg ttcagtttct tgacgccgtc tttctgccaa atgaaggcgc agttgtactg
     6241 cttgctgaag ccgctgatgt ccaggatgct gttctcggcc tcaatggcga agggcttgcc
     6301 tctcagatcg ccgtaccact tctgcagctt cagctcgcat ctgcagaact cgtccttgtc
     6361 ggcctctttc aggccctcga tcacaaagct ggccttggct ctcagccaat cggtcagagc
     6421 ggctttagat tgggcgtcct cgctccttct ttcctcttcc agcttaatgt gcttgctcag
     6481 gccttccacc ttcttgtcga ttctctccca ggcctcatcg tacactttgc cccagtcctc
     6541 gccgtgcttc ttttccaggt gcagcagcag gtcgccgaac tggtatctgg cgaacttctt
     6601 gcccttcttc cggtcctctt cgctgctcag gtaaggcagc agggcttctt gtctcttgta
     6661 gccggccagg ttctgccaga aaaccttgcc atcctctttc ttctcgttga tcagcttctt
     6721 cacgttgcag accatgtccc accaatccac ttcattggcc tgtctttcca ccagagggaa
     6781 gctagggaag cccttcagtc tctgcagtgg cttggcttcg tccctgccga tcttcagctt
     6841 ctgccacaga ttcaggttga cccaaatcac gatctgggcc accacgttgt tgtaggcctc
     6901 gatgccctct ttggtgtgag gctgtggagg cagggtgatc ttaggaaagg ccaggccgtt
     6961 agcgctggcg atatccttga ggttggccag tcttttctcg ttcttcttga tcactttctg
     7021 gtgttccagg atgatgtcct ggtacttggt caggaaggat gccacggctc ccatacaggc
     7081 gtcgctcagg gcttttccca caggtccaga ggcacagcta ttgccgccga tctgttccag
     7141 gggcttcaca gggtgattgc tctccctggt cacgtggatg ctgtagaagt ccagggctct
     7201 ctgtccgaac ttgcccaggc tgtatgtgac cagctcatcg ttggcctcg
//
