LOCUS       plasmid                 7249 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             5911..6771
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        496..875
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        876..1079
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   4854..4994
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    4238..4349
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5806..5910
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      join(6942..7249,1..281)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      5069..5524
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1127..1153
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4483..4723
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(4999..5050)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             4154..4176
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5525..5574)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5676..5711)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             4124..4146
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   354..381
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   410..437
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
       61 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg
      361 ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
      421 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggcctc
      481 tagactcgag gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      541 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      601 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      661 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      721 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      781 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      841 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      901 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      961 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     1021 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     1081 tctggctaac taccggttct agagcgctgc caccatgtcc ggatcccctg ctgccaagag
     1141 ggtcaagttg gacatgcaag agatcaagag aatcaacaag atcagaagga gactggtcaa
     1201 ggacagcaac acaaagaagg ccggcaagcg cggccccatg aaaaccctgc tcgtcagagt
     1261 gatgacccct gacctgagag agcggctgga aaacctgaga aagaagcccg agaacatccc
     1321 tcagcctatc agcaacacca gcagggccaa cctgaacaag ctgctgaccg actacaccga
     1381 gatgaagaaa gccatcctgc acgtgtactg ggaagagttc cagaaagacc ccgtgggcct
     1441 gatgagcaga gttgctcagc ccgctcctaa gaacatcgac cagagaaagc tgatccccgt
     1501 gaaggacggc aacgagagac tgacctctag cggctttgcc tgcagccagt gttgccagcc
     1561 tctgtacgtg tacaagctgg aacaagtgaa cgacaagggc aagccccaca ccaactactt
     1621 cggcagatgc aacgtgtccg agcacgagag gctgatcctg ctgtctcctc acaagcccga
     1681 ggccaacgat gagctggtca catacagcct gggcaagttc ggacagagag ccctggactt
     1741 ctacagcatc cacgtgacca gggagagcaa tcaccctgtg aagcccctgg aacagatcgg
     1801 cggcaatagc tgtgcctctg gacctgtggg aaaagccctg agcgacgcct gtatgggagc
     1861 cgtggcatcc ttcctgacca agtaccagga catcatcctg gaacaccaga aagtgatcaa
     1921 gaagaacgag aaaagactgg ccaacctcaa ggatatcgcc agcgctaacg gcctggcctt
     1981 tcctaagatc accctgcctc cacagcctca caccaaagag ggcatcgagg cctacaacaa
     2041 cgtggtggcc cagatcgtga tttgggtcaa cctgaatctg tggcagaagc tgaagatcgg
     2101 cagggacgaa gccaagccac tgcagagact gaagggcttc cctagcttcc ctctggtgga
     2161 aagacaggcc aatgaagtgg attggtggga catggtctgc aacgtgaaga agctgatcaa
     2221 cgagaagaaa gaggatggca aggttttctg gcagaacctg gccggctaca agagacaaga
     2281 agccctgctg ccttacctga gcagcgaaga ggaccggaag aagggcaaga agttcgccag
     2341 ataccagttc ggcgacctgc tgctgcacct ggaaaagaag cacggcgagg actggggcaa
     2401 agtgtacgat gaggcctggg agagaatcga caagaaggtg gaaggcctga gcaagcacat
     2461 taagctggaa gaggaaagaa ggagcgagga cgcccaatct aaagccgctc tgaccgattg
     2521 gctgagagcc aaggccagct ttgtgatcga gggcctgaaa gaggccgaca aggacgagtt
     2581 ctgcagatgc gagctgaagc tgcagaagtg gtacggcgat ctgagaggca agcccttcgc
     2641 cattgaggcc gagaacagca tcctggacat cagcggcttc agcaagcagt acaactgcgc
     2701 cttcatttgg cagaaagacg gcgtcaagaa actgaacctg tacctgatca tcaattactt
     2761 caaaggcggc aagctgcggt tcaagaagat caaacccgag gccttcgagg ctaacagatt
     2821 ctacaccgtg atcaacaaaa agtccggcga gatcgtgccc atggaagtga acttcaactt
     2881 cgacgacccc aacctgatta tcctgcctct ggccttcggc aagagacagg gcagagagtt
     2941 catctggaac gatctgctga gcctggaaac cggctctctg cgcctggcca atggcagagt
     3001 gatcgagaaa accctgtaca acaggagaac cagacaggac gagcctgctc tgtttgtggc
     3061 cctgaccttc gagagaagag aggtgctgga cagcagcaac atcaagccca tgaacctgat
     3121 cggcatcgac cggggcgaga atatccctgc tgtgatcgcc ctgacagacc ctgaaggatg
     3181 cccactgagc agattcaagg actccctggg caaccctaca cacatcctga gaatcggcga
     3241 gagctacaaa gagaagcaga ggacaatcca ggccgccaaa gaggtggaac agagaagagc
     3301 cggcggatac tctaggaagt acgccagcaa ggccaagaat ctggccgacg acatggtccg
     3361 aaacaccgcc agagatctgc tgtactacgc cgtgacacag gacgccatgc tgatcttcga
     3421 gaatctgagc agaggcttcg gccggcaggg caagagaacc tttatggccg agaggcagta
     3481 caccagaatg gaagattggc tcacagctaa actggcctac gagggactgc ccagcaagac
     3541 ctacctgtcc aaaacactgg cccagtatac ctcccgcacc tgcagcaatt gcggcttcac
     3601 catcaccagc gccgactacg acagagtgct ggaaaagctc aagaaaaccg ccaccggctg
     3661 gatgaccacc atcaacggca aagagctgaa ggttgagggc cagatcacct actacaacag
     3721 gtacaagagg cagaacgtcg tgaaggatct gagcgtggaa ctggacagac tgagcgaaga
     3781 gagcgtgaac aacgacatca gcagctggac aaagggcaga tcaggcgagg ctctgagcct
     3841 gctgaagaag aggtttagcc acagacctgt gcaagagaag ttcgtgtgcc tgaactgcgg
     3901 cttcgagaca cacgccgatg aacaggctgc cctgaacatt gccagaagct ggctgttcct
     3961 gagaagccaa gagtacaaga agtaccagac caacaagacc accggcaaca ccgacaagag
     4021 ggcctttgtg gaaacctggc agagcttcta cagaaaaaag ctgaaagaag tctggaagcc
     4081 cgccgtgaat gcattgccaa agaagaagcg gaaggtcggc agttacccat acgatgtgcc
     4141 tgactacgct tatccatacg acgtacccga ttacgcctat ccttacgacg tacctgacta
     4201 cgcctaagaa ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     4261 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4321 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4381 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct
     4441 ggggaggtac ccttttttgg tacggccgcc tagtctggcc gcgagggcct atttcccatg
     4501 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     4561 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     4621 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     4681 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgactggcgc
     4741 ttttatctga ttactttgag agccatcacc agcgactatg tcgtagtggg taaagctccc
     4801 tcttcggagg gagcatcaaa gggtcttctc gaagacatgc cgtttgcggc cgcaggaacc
     4861 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4921 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4981 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5041 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     5101 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     5161 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5221 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5281 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5341 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5401 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     5461 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5521 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5581 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5641 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5701 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5761 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5821 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5881 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5941 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6001 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6061 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6121 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6181 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6241 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6301 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6361 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6421 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6481 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6541 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6601 gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat
     6661 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6721 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6781 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6841 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6901 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6961 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7021 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7081 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7141 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7201 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagc
//
