LOCUS       plasmid                 6816 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(join(6417..6816,1..189))
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             5799..5990
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2170..4086)
                     /note="pLannotate"
                     /label="ATG20"
                     /database="swissprot"
                     /identity="99.7"
                     /match_length="99.8"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(1124..1215)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1444..2160)
                     /note="pLannotate"
                     /label="SiriusGFP"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4214..5482)
                     /note="pLannotate"
                     /label="SNX4"
                     /database="swissprot"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(1282..1329)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(266..1123)
                     /note="pLannotate"
                     /label="bla"
                     /database="swissprot"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(220..259)
                     /note="pLannotate"
                     /label="fd terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     RBS             complement(5490..5512)
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     RBS             complement(4151..4173)
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        complement(5544..5562)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(4114..4131)
                     /note="pLannotate"
                     /label="6xHis"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    6092..6231
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(1337..1386)
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5649..5759)
                     /note="pLannotate"
                     /label="mbeC (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="32.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4097..4113)
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.0"
                     /fragment="True"
                     /other="CDS"
     CDS             4186..4204
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="10.9"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
       61 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      181 ggatctcaag aagatccttt gatcttttct accggtccgt ccaaaaaaaa aggctccaaa
      241 aggagccttt aattgtatcg gtttaccaat gcttaatcag tgaagcccct atttcagcaa
      301 tctgtctatt tcgttcgtcc atagttgcct gactacccgt cgtgtagata actacgatac
      361 gggaaggctt accatctggt ccaagtgcgg caatgatacc ccgtgaacca cgctctcctg
      421 ccccagattt atcagcaata aaccagccag caggaagtgc cgaacgcagt agtggtcctg
      481 caactttatc cgcttcaagc cagtctatta attgttgccg tgaagccaga gtaagtagtt
      541 cgccagttaa tagtttgcgt aatgttgttg ccattgccgc aggcattgtg gtgtctcgct
      601 cgtcgtttgg tatggcttca ttcagttctg gttcccaacg gtcaaggcga gttacatggt
      661 ctcccatatt gtgcaaaaaa gcggttaatt ccttcggtcc accgatggtt gtcagaagta
      721 agttcgccgc agtgttatca ctcatcgtta tggcggcact acataattct cttaccgtca
      781 ttccgtccgt aaggtgcttt tctgtcactg gggagtattc aaccaagtca ttctgcgaat
      841 agtgtattcg tcgtccgagt tgctcttgcc ccgcatctac acgggataat actgctccac
      901 atagcagaac tttgaaagtg ctcatcattg gaaaacgctc ttcagggcga aaactctcaa
      961 ggattttacc gctattcaag tccagttcta tgtatcccac tcgtgccccc aattggtcct
     1021 ctgcgtcttt tactttcacg agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     1081 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     1141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     1201 agaaaaataa acaaataggg gaattaaaaa aaagcccgct cattaggcgg gctactagtc
     1261 ggatatagtt cctcctttca gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg
     1321 ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
     1381 agcagccgga tctctcgaga cgcgttcgcg aatccggatg tgaaattgtt atccgctggt
     1441 accttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     1501 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact gggtgctcag
     1561 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     1621 gtcggcgagc tgcacgctgc cgtcctcgac gttgtggcgg atcttgaagt tcgccttgat
     1681 gccgttcttc tgcttgtcgg ccgtgatata gacgttgtgg ctgttgaagt tgtactccag
     1741 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     1801 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtaggtgc cgtcgtcctt
     1861 gaaggagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     1921 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     1981 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     2041 gccgttggtg gcatcgccct cgccctcgcc gcgcacgctg aacttgtggc cgtttacgtc
     2101 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     2161 agaagaacct gcaaaatctt gatgtctttt gacttctttc caaatttcta aattttgacg
     2221 ggcataattt ctcatatatc tggaataatg ctttaaaatt tctgataaat ctacttccct
     2281 ttctttagaa aatttaggta gctggtcatt tttgatgact tttgaaatga cctccaaatc
     2341 attctcagta acttccaatg attcagacag ttgttcaatt tctttcttca aattgatact
     2401 tgcggtatgc ggatctgttt cttgatactt cacagaatct tttaccattg atgccaattt
     2461 gttaaatcca ttgaatagtt tgccaccata tgatttccca ccgctacctg tattattatt
     2521 agggcgttcc agattaatag tatgactttt agacatttcg ttgtcaatta ttttgtcaac
     2581 atccttatat tcattgtttt gcgcttccag cttctccaac tgtgctcttt tggaattcag
     2641 agacttttta atcatttcat tttgcaggta tttcaatttt ctgtatttga tcaattctct
     2701 tgcagaagtt gccatatgaa cagattcatt taaaggctca tttatattgt agtatagtct
     2761 tccaactaat ttttctaaag aaatcgagga ccccgaaaag gcattgctta agtaagaaag
     2821 ctgagcggcc aattccccaa cttgagcctg ttcagcggca aactgtgcaa atacctcacc
     2881 gatttcattg taatccgatt tcaaatcgtg ataagtcttc gtggttcgtc tattatactt
     2941 ataaatcccg ttatctaaca agttctcata ttgcttgtaa tcctgttcaa tattagtaaa
     3001 cgacttactt ctttccttat ccattttttt gtcgttggac tccttattca acaataactg
     3061 tgacgaggag cccggtattg gtaacatggc atgaattcta gtggtgttag tgggatctag
     3121 tggattacac tggaggatgc tcttcggtaa agatgaaaaa gtggacgaac tgttaacaaa
     3181 ttcatgccaa ttatggttat taggatccaa aaaatcggta attatagatg tttttgtgat
     3241 ttcttcgttc gtcaaaagtt tgttcaagaa ctcagtgagc attcttatcc tatgccgtat
     3301 taacttttca tcactattat tgaccgacgc atgtatcaca gacaatgata gatctactga
     3361 accagaacct tctgagggca gcaaatactt tgaactcgaa ccagttattg acttaccata
     3421 attctttatt gactgctttt ctggaatcgg cggtatcaac gtcataggaa acaatcttat
     3481 caagatactt ctcaaactct cgaaatcgct gtaacgtctc tgaacggtag aattttcaaa
     3541 ctgaataaca taggcgatgt aggctcttcc ttgaccttcg gaaactcttt tcgcttcaag
     3601 tatgtgtatc ctagcggact tctttctatt acttacagaa ggttcttctg acgcagaggt
     3661 ctctccgttc atggagttaa tacttccact aagacttgtt ctacatgttt ctgcattcga
     3721 ttcttggttt aaaggcaata atccctcaga gtcatcctcg taatcttcat tcgggttctt
     3781 taggttatgg cttctcatac cgggtatttc taataaagct gattttgtga atcctgttgg
     3841 cccctcctcc atgaatgggt tgtctttttc taacagggct gtgtgtacta actcagtttc
     3901 tactttgtta ttgtccccct ttccaacctt accctttttt gggctctttt ttggcgatcc
     3961 aactccattc ttagaaattt ctgcctctgc cacatactca gttgtcccat gaggtggttc
     4021 agcctttcct gtattccgag tttccgaatt caatttagca ttttcttgga catcatttaa
     4081 gtctgatgca ttggattgga agtacaggtt ttcatggtga tggtgatggt gagaagaacc
     4141 catatgtata tctccttctt aaagttaaac aaaattattg agctcgactg ggaaaaccct
     4201 ggcgaagctt ttatagacta tcgtctactt tttcccatgc ttcaagtatc ctttcgtaga
     4261 attcgatatg atggtccgct agtgagccca gagatttctt gatttcagcc gtttttacac
     4321 tttcgaaatg gtctatttcc ttcaagcatt cctgttcgaa tccatccgcc acctttttag
     4381 cgttttctaa ttcacctgtt aatgacgtta ttttgccttc tagtttattg attttttctc
     4441 tacgtgaagc ctcctgattt attcccgcta gttcttccaa tttattggca aagaaattac
     4501 tacctccgta tccagaaatc aagttatttt tttctttgat tgacctggtt aaataatcac
     4561 ttagttcttc gtagtctatc tgtttctggt ctttcaattt gatcagctgt cgcatactat
     4621 caatgtagtg ttcaagatcc ttcaaatcaa caatatattc gtaatccaga tactttgtaa
     4681 gatcttgcaa gccatatgac agctgtgtta caccttcatt aaaaatcttc aacttcgcag
     4741 caagttcctc gttttcgcct gtgaccaatt cttgtaattc ctgtaaatta ctgcccaact
     4801 ttgtatagtc ttcagacatc gaatcatttt ttttcacaac cttatgaaaa agtttatcta
     4861 tctttgtcac agtccgatct agtttatcac ttttctccct tatctcagtg aattcttcgt
     4921 tttgcttgtg aactgttttg aaggcattca taaaagcatc agtaacttca tctttatttg
     4981 gttgcaagct atcctggagg actttgcggt gagattccca actgctactc accaaaaaag
     5041 ttttgaagac ttttgattga gaaagatcgg gatgcaaaga gacccttctt aggaaatttt
     5101 gcaaactatg acatctcttt tgtgtaaatc tttggctaaa cctatcgcct gctatgtatt
     5161 gaaaaacttt cttatcaggt aatggcggga tgatgcaggt ggggaacctg ttgagtaaaa
     5221 tatcatgcag gagcaatagg tcactgtatc ttctatgtac aactataata gattctggat
     5281 cgccgctatt tgcataaaat gacgtgttgt tagtctttgt tgatatctga taactcacgt
     5341 agccagatga agagcctggg tcaccagttc ttttttgtgg atcagaaaca atgatttcaa
     5401 accaatatgg tggcttctcg gggttgcctc tggctttgtc cttagctttg cttgttaaat
     5461 cgttcttgcc tttgtctgtc atatgtatat ctccttctta aagttaaaca aaattatttc
     5521 tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg ggatcgatgc
     5581 atggtgccaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     5641 ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     5701 gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     5761 accccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     5821 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     5881 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     5941 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6001 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6061 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     6121 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     6181 ttgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     6241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6781 aggattagca gagcgaggta tgtaggcggt gctaca
//
