LOCUS       plasmid                 6152 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(4314..4742)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(4105..4209)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3244..4104)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2485..3073)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2131..2161)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1925..1949)
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    5003..5027
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(2176..2197)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(2047..2065)
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4906..4924
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(2107..2123)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(4746..4903)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4236..4286
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1973..2035)
                     /note="pLannotate"
                     /label="VESPI_VESMG (fragment)"
                     /database="swissprot"
                     /identity="81.0"
                     /match_length="31.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2210..2302)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     polyA_signal    10..27
                     /note="pLannotate"
                     /label="EF-1α poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.1"
                     /fragment="True"
                     /other="polyA_signal"
ORIGIN
        1 agaaaagcct cttgaaaatg taattctcca tcgttctttg aagacgagaa ttgagcgtct
       61 tgcaagagcc acagcaaata ccaagtcaca taaagcacca ttccgaaaca tgatgtttta
      121 tgggcctcca ggtaccggaa aaactatggt ggcgagggag attgctcgga agtcgggtct
      181 tgattatgct atgatgacag gaggagatgt tgctcctctt ggtgcacagg ctgttacaaa
      241 gatccatgaa atatttgatt gggctaagaa atcaaacaaa gggttactgc ttttcatcga
      301 tgaagctgat gctttcctat gcgaacgtaa cagcacttac atgagtgagg ctcagcgcag
      361 cgctctgaac gcgttgctct ttcgaaccgg tgatcaatcg cgggacatag tccttgtcct
      421 ggctacaaac agacctggag atctcgacag tgcagtcact gacaggatcg acgaagttat
      481 cgagtttcct ctccctggtg aagaagaacg cttcaagctc ctcaagctct atctcaacaa
      541 gtacctaatg ggtgacgaca agaaaggtga gaaagactca aaccttaaat ggagcaactt
      601 gttcaagaag aagaagtcac agaagataac cattgaagga gacctaaccg accaagtgat
      661 taaagaagct gcaaaaaaga cagaaggctt ttctggtcgt gaaatcgcta agcttgtcgc
      721 cggtgttcaa gctgcggtat atggacgaca ggattgtgtc ttggattccc aactttttga
      781 agaaattgtg gattataaga tcgaagaaca tcaccagaga atcagacttg cgactgaagg
      841 tggccaatcg tttccgtagc atcatgcatt gggtatggaa aagagttggg attctagtcc
      901 aagtgcaaat tcgtttttgc agatttgcat gcgggctttc ttgcagagag gaagtagttt
      961 atcgcattgg tggggtagtc cagcaagcct cccagaggat gtagtttcag tttcaagtag
     1021 acacattcat ttgtgagact tgacagggaa agctcaaagt ttccatcaaa ctgaccaaat
     1081 ccacctagtt tcacattatt tatgctagtt tcgaagtact ttagcgtaga attttttgcc
     1141 atctgtcatg aggccaacaa gttatgttga agagtcaatt attccttctc ggtatgtaat
     1201 atgaggctac atgccagatt gattgctgcg gaccatttga tttatcatat atatggtttt
     1261 caatcgtttt catcacgaag ttcgaactca gatgccatgc atttttaaac aattgcaaaa
     1321 aagaacatca agaattgtgc ttgtattaaa actgggataa aatcttagtt cagaaagttg
     1381 ttcgatatat attcctccat attgaacaaa aatggaattg accgtagtat tagtgttatt
     1441 gtgcaattaa gtgagccgat tggtttccct taaacacttg gaaattaact taacagattt
     1501 ataaattctc tatttcttaa acatggttca tataacttct taaaataaaa tacaattact
     1561 gaatattcta ttaaataaca tttgggttac atgcaaaaat agctgttcta tgagaacaat
     1621 tagaatatct aagcatctta tttccactcc aaacaagcac atattgaaat aataacctaa
     1681 ttcaaactag ttcttatgag catgcaattt ttaaagtttt gtcgaggatt tgagaaacat
     1741 ttaatattaa aagaatcagt atttcaaaat gaaattttct caatgacata attttttcaa
     1801 tgataatagt tgagactgtg cacttcaaat ggaagttttc tgtttaaatt tattttagaa
     1861 gaaattcttc acaataactg caggtcagag tatacgtaac taatttaaca gcaggatcct
     1921 tcacacccag ctttcttgta caaagtggtg tacgtattaa ttaagcaatc actagtgaat
     1981 tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt
     2041 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt
     2101 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2161 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2221 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2281 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2341 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2401 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2461 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2521 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2581 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2641 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2701 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2761 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2821 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2881 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     2941 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3001 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3061 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3121 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3181 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3241 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3301 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3361 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3421 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3481 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3541 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3601 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3661 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3721 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3781 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3841 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3901 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3961 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4021 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4081 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4141 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4201 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat
     4261 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     4321 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4381 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4441 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4501 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4561 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4621 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4681 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4741 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4801 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     4861 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     4921 tagggcgaat tgggcccgac gtcgcatgct cccggccgcc atggcggccg cgggaattcg
     4981 atttagccct aggtacgtaa tcacaagttt gtacaaaaaa gcaggctcat ctttttgagg
     5041 gacaagagga gcttctactt tttagtttta ttttgtctag ttttattttg tttgggaatg
     5101 gcaagtagtg cattaatgaa ggtggctagc tcagctggtg cgatcgcgtc ggcctcgtat
     5161 gtgcttgcct gtaatcctgc ctctgctgac aaaagactcg attccggatc tctgttcggc
     5221 gcatcatcct ctgctgctcc ggctcccgcg acacccactc cagtgaaaga ggaaaaacct
     5281 cgcaatgatg ctccgcgcac atccgccgct gggttcgacc cagaggcatt ggagagaggt
     5341 gccaaagcac ttcgggaaat taatgcctct aatcatgcca aaaaggtttt tgagttgatg
     5401 aagaaacagg aagagactcg gcaaatagag gaaaatgctc gtagagccga atatcaagcc
     5461 atgcaagctc aacacgagac ggagagacaa agggtaatat atgaggagca aaagaaattg
     5521 ttacagcaga cagcccagaa caaggcccag ttggctcgat atgaagatga acttacacgc
     5581 aagcggatgc agagtgaaca tgaggcaaat aggcaacgaa atcaagaatt agtgaggatg
     5641 caggaagagg cagcattgcg acaggaacag attcgaagga acacagaaga acagattcaa
     5701 gcacagaggc gtcaaacaga aaaggagatg gctgaaattg agagggaaac cattcgagtg
     5761 aaggcaatgg cagaggctga agggcgagct catgaggcta agatggctga ggacgtgaac
     5821 agaagacttc tggtcgagcg tgctaacatg gagaaggaca agtggctagc tgctattaat
     5881 accacattca gtcatattgg aggcggaata tacgcaatac tgacagatca gaacaagctt
     5941 gtcgttgccg ttggaggagc aactgccttg gcagcgggag tatatactac cagggagggg
     6001 gcccgtgtat tgtggagcca cattgatcga atactagggc aaccgtcttt ggttagggag
     6061 agcagcatgg gccgattccc atgggcaggc tcagtgtctc agtttaagaa caaacttagc
     6121 acagctgcag gggcagcagc atctgcagaa gg
//
