LOCUS       plasmid                 6152 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(2766..3194)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2557..2661)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1696..2556)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(937..1525)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(583..613)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(377..401)
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    3455..3479
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(628..649)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(499..517)
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3358..3376
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(559..575)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(3198..3355)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2688..2738
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(425..487)
                     /note="pLannotate"
                     /label="VESPI_VESMG (fragment)"
                     /database="swissprot"
                     /identity="81.0"
                     /match_length="31.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(662..754)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     polyA_signal    4614..4631
                     /note="pLannotate"
                     /label="EF-1α poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.1"
                     /fragment="True"
                     /other="polyA_signal"
ORIGIN
        1 aatacaatta ctgaatattc tattaaataa catttgggtt acatgcaaaa atagctgttc
       61 tatgagaaca attagaatat ctaagcatct tatttccact ccaaacaagc acatattgaa
      121 ataataacct aattcaaact agttcttatg agcatgcaat ttttaaagtt ttgtcgagga
      181 tttgagaaac atttaatatt aaaagaatca gtatttcaaa atgaaatttt ctcaatgaca
      241 taattttttc aatgataata gttgagactg tgcacttcaa atggaagttt tctgtttaaa
      301 tttattttag aagaaattct tcacaataac tgcaggtcag agtatacgta actaatttaa
      361 cagcaggatc cttcacaccc agctttcttg tacaaagtgg tgtacgtatt aattaagcaa
      421 tcactagtga attcgcggcc gcctgcaggt cgaccatatg ggagagctcc caacgcgttg
      481 gatgcatagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat catggtcata
      541 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      601 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      661 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      721 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1681 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1741 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1801 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1861 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1921 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1981 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2041 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2101 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2161 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2221 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2281 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2341 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2401 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2461 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2521 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2641 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgatg cggtgtgaaa
     2701 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     2761 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     2821 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     2881 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     2941 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3001 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3061 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3121 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3181 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     3241 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     3301 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     3361 tacgactcac tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc
     3421 cgcgggaatt cgatttagcc ctaggtacgt aatcacaagt ttgtacaaaa aagcaggctc
     3481 atctttttga gggacaagag gagcttctac tttttagttt tattttgtct agttttattt
     3541 tgtttgggaa tggcaagtag tgcattaatg aaggtggcta gctcagctgg tgcgatcgcg
     3601 tcggcctcgt atgtgcttgc ctgtaatcct gcctctgctg acaaaagact cgattccgga
     3661 tctctgttcg gcgcatcatc ctctgctgct ccggctcccg cgacacccac tccagtgaaa
     3721 gaggaaaaac ctcgcaatga tgctccgcgc acatccgccg ctgggttcga cccagaggca
     3781 ttggagagag gtgccaaagc acttcgggaa attaatgcct ctaatcatgc caaaaaggtt
     3841 tttgagttga tgaagaaaca ggaagagact cggcaaatag aggaaaatgc tcgtagagcc
     3901 gaatatcaag ccatgcaagc tcaacacgag acggagagac aaagggtaat atatgaggag
     3961 caaaagaaat tgttacagca gacagcccag aacaaggccc agttggctcg atatgaagat
     4021 gaacttacac gcaagcggat gcagagtgaa catgaggcaa ataggcaacg aaatcaagaa
     4081 ttagtgagga tgcaggaaga ggcagcattg cgacaggaac agattcgaag gaacacagaa
     4141 gaacagattc aagcacagag gcgtcaaaca gaaaaggaga tggctgaaat tgagagggaa
     4201 accattcgag tgaaggcaat ggcagaggct gaagggcgag ctcatgaggc taagatggct
     4261 gaggacgtga acagaagact tctggtcgag cgtgctaaca tggagaagga caagtggcta
     4321 gctgctatta ataccacatt cagtcatatt ggaggcggaa tatacgcaat actgacagat
     4381 cagaacaagc ttgtcgttgc cgttggagga gcaactgcct tggcagcggg agtatatact
     4441 accagggagg gggcccgtgt attgtggagc cacattgatc gaatactagg gcaaccgtct
     4501 ttggttaggg agagcagcat gggccgattc ccatgggcag gctcagtgtc tcagtttaag
     4561 aacaaactta gcacagctgc aggggcagca gcatctgcag aaggagaaaa gcctcttgaa
     4621 aatgtaattc tccatcgttc tttgaagacg agaattgagc gtcttgcaag agccacagca
     4681 aataccaagt cacataaagc accattccga aacatgatgt tttatgggcc tccaggtacc
     4741 ggaaaaacta tggtggcgag ggagattgct cggaagtcgg gtcttgatta tgctatgatg
     4801 acaggaggag atgttgctcc tcttggtgca caggctgtta caaagatcca tgaaatattt
     4861 gattgggcta agaaatcaaa caaagggtta ctgcttttca tcgatgaagc tgatgctttc
     4921 ctatgcgaac gtaacagcac ttacatgagt gaggctcagc gcagcgctct gaacgcgttg
     4981 ctctttcgaa ccggtgatca atcgcgggac atagtccttg tcctggctac aaacagacct
     5041 ggagatctcg acagtgcagt cactgacagg atcgacgaag ttatcgagtt tcctctccct
     5101 ggtgaagaag aacgcttcaa gctcctcaag ctctatctca acaagtacct aatgggtgac
     5161 gacaagaaag gtgagaaaga ctcaaacctt aaatggagca acttgttcaa gaagaagaag
     5221 tcacagaaga taaccattga aggagaccta accgaccaag tgattaaaga agctgcaaaa
     5281 aagacagaag gcttttctgg tcgtgaaatc gctaagcttg tcgccggtgt tcaagctgcg
     5341 gtatatggac gacaggattg tgtcttggat tcccaacttt ttgaagaaat tgtggattat
     5401 aagatcgaag aacatcacca gagaatcaga cttgcgactg aaggtggcca atcgtttccg
     5461 tagcatcatg cattgggtat ggaaaagagt tgggattcta gtccaagtgc aaattcgttt
     5521 ttgcagattt gcatgcgggc tttcttgcag agaggaagta gtttatcgca ttggtggggt
     5581 agtccagcaa gcctcccaga ggatgtagtt tcagtttcaa gtagacacat tcatttgtga
     5641 gacttgacag ggaaagctca aagtttccat caaactgacc aaatccacct agtttcacat
     5701 tatttatgct agtttcgaag tactttagcg tagaattttt tgccatctgt catgaggcca
     5761 acaagttatg ttgaagagtc aattattcct tctcggtatg taatatgagg ctacatgcca
     5821 gattgattgc tgcggaccat ttgatttatc atatatatgg ttttcaatcg ttttcatcac
     5881 gaagttcgaa ctcagatgcc atgcattttt aaacaattgc aaaaaagaac atcaagaatt
     5941 gtgcttgtat taaaactggg ataaaatctt agttcagaaa gttgttcgat atatattcct
     6001 ccatattgaa caaaaatgga attgaccgta gtattagtgt tattgtgcaa ttaagtgagc
     6061 cgattggttt cccttaaaca cttggaaatt aacttaacag atttataaat tctctatttc
     6121 ttaaacatgg ttcatataac ttcttaaaat aa
//
