LOCUS       plasmid                 6317 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      5166..5594
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        5699..5803
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      737..1325
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2954..4618)
                     /note="pLannotate"
                     /label="ATAD3A (fragment)"
                     /database="swissprot"
                     /identity="63.7"
                     /match_length="87.5"
                     /fragment="True"
                     /other="CDS"
     CDS             9..565
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.1"
                     /fragment="True"
                     /other="CDS"
     CDS             join(5804..6317,1..11)
                     /note="pLannotate"
                     /label="AmpR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="61.0"
                     /fragment="True"
                     /other="CDS"
     promoter        1649..1679
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4881..4905)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    1861..1885
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    1613..1634
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(4984..5002)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1745..1763
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1687..1703
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             5005..5162
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5622..5672)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1775..1837
                     /note="pLannotate"
                     /label="VESPI_VESMG (fragment)"
                     /database="swissprot"
                     /identity="81.0"
                     /match_length="31.3"
                     /fragment="True"
                     /other="CDS"
     CDS             1508..1600
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 caaacgacga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
       61 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      721 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1381 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1441 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1501 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1561 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     1621 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1681 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1741 agctatttag gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc
     1801 ccatatggtc gacctgcagg cggccgcgaa ttcactagtg attgcttaat taatacgtac
     1861 accactttgt acaagaaagc tgggtgtgaa ggatcctgct gttaaattag ttacgtatac
     1921 tctgacctgc agttattgtg aagaatttct tctaaaataa atttaaacag aaaacttcca
     1981 tttgaagtgc acagtctcaa ctattatcat tgaaaaaatt atgtcattga gaaaatttca
     2041 ttttgaaata ctgattcttt taatattaaa tgtttctcaa atcctcgaca aaactttaaa
     2101 aattgcatgc tcataagaac tagtttgaat taggttatta tttcaatatg tgcttgtttg
     2161 gagtggaaat aagatgctta gatattctaa ttgttctcat agaacagcta tttttgcatg
     2221 taacccaaat gttatttaat agaatattca gtaattgtat tttattttaa gaagttatat
     2281 gaaccatgtt taagaaatag agaatttata aatctgttaa gttaatttcc aagtgtttaa
     2341 gggaaaccaa tcggctcact taattgcaca ataacactaa tactacggtc aattccattt
     2401 ttgttcaata tggaggaata tatatcgaac aactttctga actaagattt tatcccagtt
     2461 ttaatacaag cacaattctt gatgttcttt tttgcaattg tttaaaaatg catggcatct
     2521 gagttcgaac ttcgtgatga aaacgattga aaaccatata tatgataaat caaatggtcc
     2581 gcagcaatca atctggcatg tagcctcata ttacataccg agaaggaata attgactctt
     2641 caacataact tgttggcctc atgacagatg gcaaaaaatt ctacgctaaa gtacttcgaa
     2701 actagcataa ataatgtgaa actaggtgga tttggtcagt ttgatggaaa ctttgagctt
     2761 tccctgtcaa gtctcacaaa tgaatgtgtc tacttgaaac tgaaactaca tcctctggga
     2821 ggcttgctgg actaccccac caatgcgata aactacttcc tctctgcaag aaagcccgca
     2881 tgcaaatctg caaaaacgaa tttgcacttg gactagaatc ccaactcttt tccataccca
     2941 atgcatgatg tcaggatggg gagggctcgt ccccacgccc aggcccttcc gccttcagcc
     3001 agcacatctt ctgctggtgc tgctggacag catcttgcac gcgggtgtcc atcatggcct
     3061 cggtcaggac cccgtcctcg gaggcatacg ccgtggcctg ccaggacacg gccagctgag
     3121 cgatctcccg gcccgacatg ccctccgtca gccgagcgac ctccgagcac ttcctcccgt
     3181 agtcaaactg ggccagcttc aggcgctgct ttccttctgt ggccggctta agaacatact
     3241 tgtcaaaata cattctcacc aggcgctccc gttcctcctg ccctggcagg tcgaagtgga
     3301 ccatctcatt gatgcggtca ttgatggccc agtcaaactg ctctggttgg ttgctggcca
     3361 ggaccagcat gaacttgttg ctgtgctggc ccgtgcggta caggaaggcg ttcagtgtgg
     3421 ccctgaggtc ctcgcttatc ttctcggtgg ctcgcttccg aaggaaggcg tccgcttcat
     3481 ccacaaagag caggaggccg cgccggctgg tattggccca gtcaaagagc ttgtgcatgg
     3541 cggtcacgcc ttcccgcccc atgggggcca cgtccccgcc tgtcatgatg gcgtagtcca
     3601 tgcctgagtg cagggcgagt ttcttggcaa acagcgtctt cccggtgcct ggtggcccgt
     3661 acatcaggat gttcctgtac aggctgcggt tcttcttggt gttccttgtt gctatggcga
     3721 tgtcgcgcac ccgtgcttcc aggctgggac tgagcacaac accctccagc gcgtcctggg
     3781 gtcgactgag gagccgccgg ctgacctgga tggggtgccg cagcgcctca agcaccgtga
     3841 tgctgctctc tctaaccaaa gacggttgcc ctagtattcg atcaatgtgg ctccacaata
     3901 cacgggcccc ctccctggta gtatatactc ccgctgccaa ggcagttgct cctccaacgg
     3961 caacgacaag cttgttctga tctgtcagta ttgcgtatat tccgcctcca atatgactga
     4021 atgtggtatt aatagcagct agccacttgt ccttctccat gttagcacgc tcgaccagaa
     4081 gtcttctgtt cacgtcctca gccatcttag cctcatgagc tcgcccttca gcctctgcca
     4141 ttgccttcac tcgaatggtt tccctctcaa tttcagccat ctccttttct gtttgacgcc
     4201 tctgtgcttg aatctgttct tctgtgttcc ttcgaatctg ttcctgtcgc aatgctgcct
     4261 cttcctgcat cctcactaat tcttgatttc gttgcctatt tgcctcatgt tcactctgca
     4321 tccgcttgcg tgtaagttca tcttcatatc gagccaactg ggccttgttc tgggctgtct
     4381 gctgtaacaa tttcttttgc tcctcatata ttaccctttg tctctctgtc tcgtgttgag
     4441 cttgcatggc ttgatattcg gctctacgag cattttcctc tatttgccga gtctcttcct
     4501 gtttcttcat caactcaaaa acctttttgg catgattaga ggcattaatt tcccgaagtg
     4561 ctttggcacc tctctccaat gcctctgggt cgaacccagc ggcggatgtg cgcggagcat
     4621 cattgcgagg tttttcctct ttcactggag tgggtgtcgc gggagccgga gcagcagagg
     4681 atgatgcgcc gaacagagat ccggaatcga gtcttttgtc agcagaggca ggattacagg
     4741 caagcacata cgaggccgac gcgatcgcac cagctgagct agccaccttc attaatgcac
     4801 tacttgccat tcccaaacaa aataaaacta gacaaaataa aactaaaaag tagaagctcc
     4861 tcttgtccct caaaaagatg agcctgcttt tttgtacaaa cttgtgatta cgtacctagg
     4921 gctaaatcga attcccgcgg ccgccatggc ggccgggagc atgcgacgtc gggcccaatt
     4981 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg
     5041 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     5101 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     5161 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5221 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5281 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5341 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5401 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5461 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5521 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5581 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
     5641 cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg
     5701 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5761 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5821 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5881 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5941 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6001 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6061 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6121 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6181 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6241 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6301 gctgaatgaa gccatac
//
