LOCUS       plasmid                 6101 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      677..1105
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1210..1314
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1315..2175
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2346..2934
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(join(4590..6101,1..105))
                     /note="pLannotate"
                     /label="Atad3 (fragment)"
                     /database="swissprot"
                     /identity="62.4"
                     /match_length="91.2"
                     /fragment="True"
                     /other="CDS"
     promoter        3258..3288
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3470..3494
                     /note="pLannotate"
                     /label="attB2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(392..416)
                     /note="pLannotate"
                     /label="attB1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    3222..3243
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        3354..3372
                     /note="pLannotate"
                     /label="SP6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(495..513)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3296..3312
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             516..673
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1133..1183)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.2"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3384..3446
                     /note="pLannotate"
                     /label="VESPI_VESMG (fragment)"
                     /database="swissprot"
                     /identity="81.0"
                     /match_length="31.3"
                     /fragment="True"
                     /other="CDS"
     CDS             3117..3209
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ctatttgccg agtttcctca tcagttcaaa tacttttttg gcctgaggcg aagaattaat
       61 ttcccgcaat gccttggctc ctcgctcaag tgcctcagga tcgaaacctg cagcggtagt
      121 gcgtgggtta tcgttccgca ctcgcgtctc ctccttgggc gcgctagcgg ggggaggtcc
      181 cggtggcgcc gcttgctggg gcggcggaga gctgaagggg gggaagttga aagggccatc
      241 ggcatacgcg tgctctactg ccaaggcagc ggacgccaag gctgacacga gcccggcagc
      301 tcgggccttt gccattgcca ttcccaaaca aaataaaact agacaaaata aaactaaaaa
      361 gtagaagctc ctcttgtccc tcaaaaagat gagcctgctt ttttgtacaa acttgtgatt
      421 acgtacctag ggctaaatcg aattcccgcg gccgccatgg cggccgggag catgcgacgt
      481 cgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
      541 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      601 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      661 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     1021 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     1081 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     1141 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg
     1201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2101 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2161 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2461 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2941 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3001 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3061 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     3241 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     3301 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctatttagg
     3361 tgacactata gaatactcaa gctatgcatc caacgcgttg ggagctctcc catatggtcg
     3421 acctgcaggc ggccgcgaat tcactagtga ttgcttaatt aatacgtaca ccactttgta
     3481 caagaaagct gggtgtgaag gatcctgctg ttaaattagt tacgtatact ctgacctgca
     3541 gttattgtga agaatttctt ctaaaataaa tttaaacaga aaacttccat ttgaagtgca
     3601 cagtctcaac tattatcatt gaaaaaatta tgtcattgag aaaatttcat tttgaaatac
     3661 tgattctttt aatattaaat gtttctcaaa tcctcgacaa aactttaaaa attgcatgct
     3721 cataagaact agtttgaatt aggttattat ttcaatatgt gcttgtttgg agtggaaata
     3781 agatgcttag atattctaat tgttctcata gaacagctat ttttgcatgt aacccaaatg
     3841 ttatttaata gaatattcag taattgtatt ttattttaag aagttatatg aaccatgttt
     3901 aagaaataga gaatttataa atctgttaag ttaatttcca agtgtttaag ggaaaccaat
     3961 cggctcactt aattgcacaa taacactaat actacggtca attccatttt tgttcaatat
     4021 ggaggaatat atatcgaaca actttctgaa ctaagatttt atcccagttt taatacaagc
     4081 acaattcttg atgttctttt ttgcaattgt ttaaaaatgc atggcatctg agttcgaact
     4141 tcgtgatgaa aacgattgaa aaccatatat atgataaatc aaatggtccg cagcaatcaa
     4201 tctggcatgt agcctcatat tacataccga gaaggaataa ttgactcttc aacataactt
     4261 gttggcctca tgacagatgg caaaaaattc tacgctaaag tacttcgaaa ctagcataaa
     4321 taatgtgaaa ctaggtggat ttggtcagtt tgatggaaac tttgagcttt ccctgtcaag
     4381 tctcacaaat gaatgtgtct acttgaaact gaaactacat cctctgggag gcttgctgga
     4441 ctaccccacc aatgcgataa actacttcct ctctgcaaga aagcccgcat gcaaatctgc
     4501 aaaaacgaat ttgcacttgg actagaatcc caactctttt ccatacccaa tgcatgatgt
     4561 caggatgggg agggctcgtc cccacgccca ggcccttccg ccttcagcca gcacatcttc
     4621 tgctggtgct gctggacagc atcttgcacg cgggtgtcca tcatggcctc ggtcaggacc
     4681 ccgtcctcgg aggcatacgc cgtggcctgc caggacacgg ccagctgagc gatctcccga
     4741 cccgacatgc cctccgtcag ccgagcgacc tccgagcact tcctcccgta gtcaaactgg
     4801 gccagcttca ggcgctgctt tccttctgtg gccggcttaa gaacatactt gtcaaaatac
     4861 attctcacca ggcgctcccg ttcctcctgc cctggcaggt cgaagtggac catctcattg
     4921 atgcggtcat tgatggccca gtcaaactgc tctggttggt tgctggccag gaccagcatg
     4981 aacttgttgc tgtgctggcc cgtgcggtac aggaaggcgt tcagtgtggc cctgaggtcc
     5041 tcgcttatct tctcggtggc tcgcttccga aggaaggcgt ccgcttcatc cacaaagagc
     5101 aggaggccgc gccggctggt attggcccag tcaaagagct tgtgcatggc ggtcacgcct
     5161 tcccgcccca tgggggccac gtccccgcct gtcatgatgg cgtagtccat gcctgagtgc
     5221 agggcgagtt tcttggcaaa cagcgtcttc ccggtgcctg gtggcccgta catcaggatg
     5281 ttcctgtaca ggctgcggtt cttcttggtg ttccttgttg ctatggcgat gtcgcgcacc
     5341 cgtgcttcca ggctgggact gagcacaaca ccctccagcg cgtcctgggg tcgactgagg
     5401 agccgccggc tgacctggat ggggtgccgc agcgcctcaa gcaccgtgat gctgctctct
     5461 ctaaccaaag acggttgccc tagtattcga tcaatgtggc tccacaatac acgggccccc
     5521 tccctggtag tatatactcc cgctgccaag gcagttgctc ctccaacggc aacgacaagc
     5581 ttgttctgat ctgtcagtat tgcgtatatt ccgcctccaa tatgactgaa tgtggtatta
     5641 atagcagcta gccacttgtc cttctccatg ttagcacgct cgaccagaag tcttctgttc
     5701 acgtcctcag ccatcttagc ctcatgagct cgcccttcag cctctgccat tgccttcact
     5761 cgaatggttt ccctctcaat ttcagccatc tccttttctg tttgacgcct ctgtgcttga
     5821 atctgttctt ctgtgttcct tcgaatctgt tcctgtcgca atgctgcctc ttcctgcatc
     5881 ctcactaatt cttgatttcg ttgcctattt gcctcatgtt cactctgcat ccgcttgcgt
     5941 gtaagttcat cttcatatcg agccaactgg gccttgttct gggctgtctg ctgtaacaat
     6001 ttcttttgct cctcatatat taccctttgt ctctccgtct cgtgttgagc ttgcatggct
     6061 tgatattcgg ctctacgagc attttcctct atttgccgag t
//
