LOCUS       plasmid                 6893 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(1336..1724)
                     /note="pLannotate"
                     /label="R6K γ ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     oriT            1895..2004
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     CDS             complement(2135..2995)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3483..4211
                     /note="pLannotate"
                     /label="ermC'"
                     /database="swissprot"
                     /identity="70.4"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(6144..6201)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="53.7"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(57..111)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="98.2"
                     /match_length="50.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2037..2082
                     /note="pLannotate"
                     /label="traJ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.4"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2996..3012)
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="26.6"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(1303..1324)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.4"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 ttgattgttg cagccaatgc acaaatgctg ttccatcact ggaagatagg caattagtcg
       61 acggtatcga taagcttgat atcgaatatc ctgcagcccg ggggatccac ttgttacatt
      121 tctttttttc gatttgcata accgttattt gggtaatata tccgctctct tcatcctccg
      181 tctctacgat gacattctgg ataatcgttt cttttctgcc cgatatggtg cagtccttgg
      241 caataagaat aaaagtcgtt atatttattg ttgttgacaa taaccagcgt gttcgggtta
      301 ttattaaaat agttgtccac gacataaccg taaacaatgc cctggtttta ccgtctgcag
      361 tgttctgcaa cttctctttg atgtctaaac cgatgagcac cattacatcg gtagaatcat
      421 tctccgagaa gggcagaatg ttttatccgg tgtctgatgg gataccgggt attctccgta
      481 ttaagcaata cttcgtactc tccgggttcg gtataagcat aaagagccgt tttttcacgg
      541 gaatcaatca tccccgtctc tccgaactcc cagcgccatt gttcgtcgtg tccttcgcaa
      601 cggaagatga tattttctcc ctgaatggct tccttgggag ctatgatatg aaccagatgc
      661 aggtcttccg cattggtctt ctcttttact ctcacatcaa agtagcgttc cagatttccg
      721 ttgacggtca gtcgtatttt gtacacccct ttttgcttga accgatggta tccgctgcgt
      781 tgtgtggatg tctcgttgtt tccgaactcc cagtaccaga ttctcgctcc cgacgtggaa
      841 tcggaaaaaa atatttcttc ccccacgaca gggtcagacg gagttacagt agcctgaatc
      901 ggctgcttct gcgagcaagc tctcattacc agcagaaaaa acataataag tatgataacg
      961 gagataaaga tatagagaac cttcgtattt aggaatttat tcattgttaa aaacagattt
     1021 ggagtgcaaa gttacgacaa ataatttgtt aacatacata tttaggcaac tttttcacta
     1081 tattttcaac taataaaaca aaaaagaatg ccgacaagtt gtgtgacggc atcctttttc
     1141 tatttcatta gcttatccta caatcttctt accttaattt ataatttatt cctgagtcag
     1201 atcaactgca aagtatccgc gtttaccgga tgggaataca cctgtcagga acaatacctg
     1261 attcggagac ttaacagcag ctttcattgc ttcttccaga tcagcggccg ccaccgcggt
     1321 ggaggggaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
     1381 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
     1441 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
     1501 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
     1561 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
     1621 tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag
     1681 cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca
     1741 tcgtggccgg atcaattccg ttttccgctg cataaccctg cttcggggtc attatagcga
     1801 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     1861 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     1921 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     1981 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     2041 ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg atccccctcg
     2101 aattgacgcg ttatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3001 tcctttttca atcatggccg cgggattaaa agtcggggat tggtgaacaa aaaggtgttt
     3061 ctctctttaa gagaaatatc gttttgctaa acagttgata ttgaggtatc attttatcgt
     3121 aaaagacatt tttgctcaac aattgcttga cggaaatcaa caaattttag cattttgtaa
     3181 aaaagtcgct atataatttg gtgaattgga gttattttca tatttttgca tcccgaagag
     3241 tttctcttaa agagagaaac atcttttgca taccttttcc gaccgaattt ttatgtcgta
     3301 aagaggggct ttgcaggggg tggactcaga aagatgagaa tagatgacta ttgtagttga
     3361 aacacataga aagttgctga tatacagacc gatacgcata tcgggatgaa ccatgagtac
     3421 gttcttttct caaaaaacat aaatattcga aaagagatgc aataaattaa ggagaggtta
     3481 taatgaacaa agtaaatata aaagatagtc aaaattttat tacttcaaaa tatcacatag
     3541 aaaaaataat gaattgcata agtttagatg aaaaagataa catctttgaa ataggtgcag
     3601 ggaaaggtca ttttactgct ggattggtaa agagatgtaa ttttgtaacg gcgatagaaa
     3661 ttgattctaa attatgtgag gtaactcgta ataagctctt aaattatcct aactatcaaa
     3721 tagtaaatga tgatatactg aaatttacat ttcctagcca caatccatat aaaatatttg
     3781 gcagcatacc ttacaacata agcacaaata taattcgaaa aattgttttt gaaagttcag
     3841 ccacaataag ttatttaata gtggaatatg gttttgctaa aatgttatta gatacaaaca
     3901 gatcactagc attgctgtta atggcagagg tagatatttc tatattagca aaaattccta
     3961 ggtattattt ccatccaaaa cctaaagtgg atagcacatt aattgtatta aaaagaaagc
     4021 cagcaaaaat ggcatttaaa gagagaaaaa aatatgaaac ttttgtaatg aaatgggtta
     4081 acaaagagta cgaaaaactg tttacaaaaa atcaatttaa taaagcttta aaacatgcga
     4141 gaatatatga tataaacaat attagtttcg aacaatttgt atcgctattt aatagttata
     4201 aaatatttaa cggctaaaaa caataggcca catgcaactg taaatgttta cgcgtcctcg
     4261 gtaccaacaa atactttcag gacgatgtaa agtcctgaga aatcaaactg aatggataga
     4321 taaatattag tcttttctca tcggattctt ttaatatcgc tactctttta ttatgtagtg
     4381 gttgtattat cttaaataaa acaaaaacac ccgactttac attcttgggt gtaaaacagg
     4441 gtgtttgttt tgcaatctgc tgattttcag cgttttttgc ggagagacag gctctcgaac
     4501 ttatactttc acagaatatc ataaaactgc aaacgactga taatcaagtg taaattacaa
     4561 tatagagtaa aagtaaatgt ttctaaatgt cttttgctat ctcaataatt gggtgtatat
     4621 ttgggtgtag aatttcaaac gcacccaatt atgaatatca agcgcaacat catttttgca
     4681 ttggagagcc ggaaaaagaa cggtgtgcca atcgtagaga acgtacccat ccgtatgcgt
     4741 gtcatctttg ccagccaacg tatcgagttt acaacgggct accggattga cgtagccaaa
     4801 tgggatgcag ataagcagcg ggtaaagaac ggatgtacca acaagctaaa gcaaagtgca
     4861 gccgaaatca atacggactt gctgaaatac tatgccgaaa tccagaatat tttcaaggaa
     4921 tttgaggtgc aggaggtcat gccaacgacc caacagttga aggaagcttt caacatgaga
     4981 atgaaagaca ccagtgaaga acagccggaa gaagcccctg tcagcttttg ggaggtgttc
     5041 gatgagtttg taaaagagtg cggtaaccag aataactgga cggcatccac ctatgaaaaa
     5101 tttgcagcag tgaggaacca cctcaaagag ttcaaggagg atgcaacgtt caactatttc
     5161 aacgagtttg gattgaacga atacgtcaac ttcctgcgtg acaccaagga tatgagaaac
     5221 agcaccatcg gcaagcaaat gggattcctc aaatggttcc tgcgctggag cttcaagaaa
     5281 ggacatcatc agaacattgc atacgatacg ttcaaaccga aactgaaaac cacctcgaaa
     5341 aaagtaatct tcctgacttg ggatgaactg aacaagctga aagactacca gatacccaag
     5401 gataagcaat acctggaacg tgtgcgtgat gttttcctgt tctgctgctt tacgagtttg
     5461 cggtattcgg atgttcgcaa tctgaaaaga agcgatgtga agtccgacca catcgaaata
     5521 accacagtca agactgccga cagcctgacg attgaactga acaaatacag caaagccata
     5581 ctggacaaat acaaggacat ccatttcgag aattacatgg ctctgcccgt catcagcaac
     5641 cagaagatga acgattacct gaaagagctg ggcgaactgg cagaaatcaa cgagcctgta
     5701 cgggaaacct actacaaggg aaatgaacgt attgatgaag tcacacccaa atacgctttg
     5761 ctcagtaccc atgcaggaaa aaggacattc atctgcaatg cgctggctct cggaatcccg
     5821 gcacaggtgg tcatgaaatg gacgggacac agcgactaca aagctatgaa accctacatt
     5881 gacatagcgg atgatattaa ggcaaatgcc atgaacaagt ttaatcaact ataaaagtat
     5941 caaacagttt catagatagt taccaatgac tatatttgta gaaattatca tcacaatatg
     6001 agcaacaagg aatccaacgt agagaaaacg aattttgatg cattcataca agcggtcggt
     6061 tcggaaatag aacaggcaca agtaaggctg attgttgcag ccaatgcaca aatgctgttc
     6121 catcactgga agataggcaa ttagtcgacg gtatcgataa gcttgatatc gaattcctgc
     6181 agcccggggg atccactagt ttcatttctt tttttcgatt tgcataaccg ttatttgggt
     6241 aatatatccg ctctcttcat cctccgtctc tacgatgaca ttctggataa tcgtttcttt
     6301 tctgccgata tggtgcagtc cttggcaata agaataaaag tcgttatatt tattgttgtt
     6361 gacaataacc agcgtgttcg ggttattatt aaaatacttg tccacgacat agttgtagtt
     6421 tactgctgcg ggtttaccgt ctgcaatgtt ctgcaacttc tctttgatgt ctaaaccgat
     6481 gagcaccatt acatcggtag aatcattctc cgaatagtag ggcagaatgt ttatccggtg
     6541 tctgatggga taccgggtat tctccgtatt aagcaatact tcgtactctc cgggttcggt
     6601 ataagcataa agagccgttt tttcacggga atcaatcatc cccgtctctc cgaactccca
     6661 gcgccattgt tcgtcgtgtc cttcgcaacg gaagatgata ttttctccct gaatggcttc
     6721 cttgggagct atgatatgaa ccagatgcag gtcttccgca ttggtcttct cttactctca
     6781 catcaaagaa gcggcgcacg aaaaacgcga aaagcgtttc acgataaatg cgaaaactac
     6841 gtcctgaagt aagtgtgggt gggttaaaca cccgatttta taactgaacg aag
//
