LOCUS       plasmid                 7570 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      complement(1232..1620)
                     /note="pLannotate"
                     /label="R6K γ ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     oriT            1791..1900
                     /note="pLannotate"
                     /label="oriT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
     CDS             complement(2031..2891)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3379..4107
                     /note="pLannotate"
                     /label="ermC'"
                     /database="swissprot"
                     /identity="70.4"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(6040..6100)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="56.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1933..1978
                     /note="pLannotate"
                     /label="traJ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.4"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2892..2908)
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="26.6"
                     /fragment="True"
                     /other="promoter"
     misc_feature    complement(1199..1220)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.4"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 ttgcttgatt tgatttccac gggtataaga gattctcttt ccgtcgatgt tgaacacgtg
       61 gttgtccttc ttgtcaataa ccggttctaa tgttccgctg acgaacgagc aggtggaatc
      121 ggtatggtaa ggatatatca taggagcata tttcagcgtc ttttgcaaag ccggcactct
      181 ggaaaacgcc gttcctgtga acacctgatg tccgatttct tgcagcatga cggcgggaat
      241 ccatccgata acctgctcac cgattttatc ggcagagaga gaaggagttt tcgataccaa
      301 tacactgcgt ctgtcaacag aatacttgag cgcgtatata atagcgtgga tacccaccac
      361 gccggtttga ttctgtagct ccggatcgcc aaacactttc aatgaatcgg cggttgtaaa
      421 ataagttgcg gattgcatga ttgccgtggt gtcggtaatc gcagtcaaca atttgtcatg
      481 cagtcccgac tgtacatcgg taatggaaga aggtgaaagt atcagtcggg agcggtgcat
      541 ccagcccaca tattccgcct ttttgcgttg ggtcatacgg ttgtgcttga tatttgtggg
      601 gttatattta atcaaccgca aataatctcc tttggtcttg ataactaaga acgtatccag
      661 taatcctacc tcggcagact ttaccctgcc tcccggattg acataggcgg cattctccac
      721 cctgtccgaa tagacaatcc acggtttgcg gtgggcagcg cttctgggtg cctttatccc
      781 ctctatggaa taattttcag aatattcacg aggaagcttc cgcagttgag tgaagcggtg
      841 tacgggagca caagccgata aaacgacgat agaaatcaat aggacaaata actttctcat
      901 tgttaaaaac agatttggag tgcaaagtta cgacaaataa tttgttaaca tacatattta
      961 ggcaactttt tcactatatt ttcaactaat aaaacaaaaa agaatgccga caagttgtgt
     1021 gacggcatcc tttttctatt tcattagctt atcctacaat cttcttacct taatttataa
     1081 tttattcctg agtcagatca actgcaaagt atccgcgttt accggatggg aatacacctg
     1141 tcaggaacaa tacctgattc ggagacttaa cagcagcttt cattgcttct tccagatcag
     1201 cggccgccac cgcggtggag gggaattccc atgtcagccg ttaagtgttc ctgtgtcact
     1261 caaaattgct ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc
     1321 cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa
     1381 cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg
     1441 agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta
     1501 gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt
     1561 acgtgaaaca tgagagctta gtacgtacta tcaacaggtt gaactgctga tcttcagatc
     1621 ctctacgccg gacgcatcgt ggccggatca attccgtttt ccgctgcata accctgcttc
     1681 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
     1741 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
     1801 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
     1861 gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat
     1921 gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcacg
     1981 gaattgatcc ccctcgaatt gacgcgttat gagtaaactt ggtctgacag ttaccaatgc
     2041 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2101 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2161 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2221 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2281 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2341 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     2401 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     2461 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     2521 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2581 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2641 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2701 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2761 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2821 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2881 tgaatactca tactcttcct ttttcaatca tggccgcggg attaaaagtc ggggattggt
     2941 gaacaaaaag gtgtttctct ctttaagaga aatatcgttt tgctaaacag ttgatattga
     3001 ggtatcattt tatcgtaaaa gacatttttg ctcaacaatt gcttgacgga aatcaacaaa
     3061 ttttagcatt ttgtaaaaaa gtcgctatat aatttggtga attggagtta ttttcatatt
     3121 tttgcatccc gaagagtttc tcttaaagag agaaacatct tttgcatacc ttttccgacc
     3181 gaatttttat gtcgtaaaga ggggctttgc agggggtgga ctcagaaaga tgagaataga
     3241 tgactattgt agttgaaaca catagaaagt tgctgatata cagaccgata cgcatatcgg
     3301 gatgaaccat gagtacgttc ttttctcaaa aaacataaat attcgaaaag agatgcaata
     3361 aattaaggag aggttataat gaacaaagta aatataaaag atagtcaaaa ttttattact
     3421 tcaaaatatc acatagaaaa aataatgaat tgcataagtt tagatgaaaa agataacatc
     3481 tttgaaatag gtgcagggaa aggtcatttt actgctggat tggtaaagag atgtaatttt
     3541 gtaacggcga tagaaattga ttctaaatta tgtgaggtaa ctcgtaataa gctcttaaat
     3601 tatcctaact atcaaatagt aaatgatgat atactgaaat ttacatttcc tagccacaat
     3661 ccatataaaa tatttggcag cataccttac aacataagca caaatataat tcgaaaaatt
     3721 gtttttgaaa gttcagccac aataagttat ttaatagtgg aatatggttt tgctaaaatg
     3781 ttattagata caaacagatc actagcattg ctgttaatgg cagaggtaga tatttctata
     3841 ttagcaaaaa ttcctaggta ttatttccat ccaaaaccta aagtggatag cacattaatt
     3901 gtattaaaaa gaaagccagc aaaaatggca tttaaagaga gaaaaaaata tgaaactttt
     3961 gtaatgaaat gggttaacaa agagtacgaa aaactgttta caaaaaatca atttaataaa
     4021 gctttaaaac atgcgagaat atatgatata aacaatatta gtttcgaaca atttgtatcg
     4081 ctatttaata gttataaaat atttaacggc taaaaacaat aggccacatg caactgtaaa
     4141 tgtttacgcg tcctcggtac caacaaatac tttcaggacg atgtaaagtc ctgagaaatc
     4201 aaactgaatg gatagataaa tattagtctt ttctcatcgg attcttttaa tatcgctact
     4261 cttttattat gtagtggttg tattatctta aataaaacaa aaacacccga ctttacattc
     4321 ttgggtgtaa aacagggtgt ttgttttgca atctgctgat tttcagcgtt ttttgcggag
     4381 agacaggctc tcgaacttat actttcacag aatatcataa aactgcaaac gactgataat
     4441 caagtgtaaa ttacaatata gagtaaaagt aaatgtttct aaatgtcttt tgctatctca
     4501 ataattgggt gtatatttgg gtgtagaatt tcaaacgcac ccaattatga atatcaagcg
     4561 caacatcatt tttgcattgg agagccggaa aaagaacggt gtgccaatcg tagagaacgt
     4621 acccatccgt atgcgtgtca tctttgccag ccaacgtatc gagtttacaa cgggctaccg
     4681 gattgacgta gccaaatggg atgcagataa gcagcgggta aagaacggat gtaccaacaa
     4741 gctaaagcaa agtgcagccg aaatcaatac ggacttgctg aaatactatg ccgaaatcca
     4801 gaatattttc aaggaatttg aggtgcagga ggtcatgcca acgacccaac agttgaagga
     4861 agctttcaac atgagaatga aagacaccag tgaagaacag ccggaagaag cccctgtcag
     4921 cttttgggag gtgttcgatg agtttgtaaa agagtgcggt aaccagaata actggacggc
     4981 atccacctat gaaaaatttg cagcagtgag gaaccacctc aaagagttca aggaggatgc
     5041 aacgttcaac tatttcaacg agtttggatt gaacgaatac gtcaacttcc tgcgtgacac
     5101 caaggatatg agaaacagca ccatcggcaa gcaaatggga ttcctcaaat ggttcctgcg
     5161 ctggagcttc aagaaaggac atcatcagaa cattgcatac gatacgttca aaccgaaact
     5221 gaaaaccacc tcgaaaaaag taatcttcct gacttgggat gaactgaaca agctgaaaga
     5281 ctaccagata cccaaggata agcaatacct ggaacgtgtg cgtgatgttt tcctgttctg
     5341 ctgctttacg agtttgcggt attcggatgt tcgcaatctg aaaagaagcg atgtgaagtc
     5401 cgaccacatc gaaataacca cagtcaagac tgccgacagc ctgacgattg aactgaacaa
     5461 atacagcaaa gccatactgg acaaatacaa ggacatccat ttcgagaatt acatggctct
     5521 gcccgtcatc agcaaccaga agatgaacga ttacctgaaa gagctgggcg aactggcaga
     5581 aatcaacgag cctgtacggg aaacctacta caagggaaat gaacgtattg atgaagtcac
     5641 acccaaatac gctttgctca gtacccatgc aggaaaaagg acattcatct gcaatgcgct
     5701 ggctctcgga atcccggcac aggtggtcat gaaatggacg ggacacagcg actacaaagc
     5761 tatgaaaccc tacattgaca tagcggatga tattaaggca aatgccatga acaagtttaa
     5821 tcaactataa aagtatcaaa cagtttcata gatagttacc aatgactata tttgtagaaa
     5881 ttatcatcac aatatgagca acaaggaatc caacgtagag aaaacgaatt ttgatgcatt
     5941 catacaagcg gtcggttcgg aaatagaaca ggcacaagta aggctgattg ttgcagccaa
     6001 tgcacaaatg ctgttccatc actggaagat aggcaattag tcgacggtat cgataagctt
     6061 gatatcgaat tcctgcagcc cgggggatcc actagttcta cggtaacagt tctgaagcta
     6121 tgtagtaata gctttgtcct tccatcgtta ttttttcttc tccgtatttc ttagccatat
     6181 tttctttcct ctctttaaaa tattgaataa gtccgtccaa ctctttgtcg gttaactgtt
     6241 ttttcttttt gagatccttc accgttatgt catcaagcac agggctgttg gccggaactc
     6301 caaatatctg actgtgtgca taagaaagag aatatcgcct gatttccttt cgcttatttt
     6361 tgcagatccg gcagttgcgc agttctgaca tataaaagcg ataaaggtgg cggcgtattt
     6421 ttccggtaga tacatacacc gtatctgctt tgctttcggg atttgcgggt gatgcgccga
     6481 gagtttctac cttatcgggg cgtacccttt cccttaaata gcggcacaac tgtttaactt
     6541 tacctttctt tggcttattc atgtccttca catccacttc tatggcacag agtgtttcca
     6601 agcgttctat tgtctgtttc agttccgggt cggagagtag caggtaatag cgcatcaaac
     6661 tgtccggaac ggtaattctt tctgttttcc tataccatat cggagcaaca tcaccaaata
     6721 tttttttaaa ctccttattc ggttttaccc cttgaggcag atgataggtt gcaatcagaa
     6781 gactgtctaa acggtcttta ccgttgccaa ccgtggcaaa tgcacggtct atgctttcag
     6841 agagcagttg gtgatccgct tttatctgtg ttacgatgga atccaccgct ccggcaaaca
     6901 attccattgg cagggtttca cctttctccg gaaacaggaa accgccttgt gtcatgcagg
     6961 catagggata gtcgagcatg agaaaattcg ggcctgcttc ccgcagcagg ttgctgcggc
     7021 aaagctggtc gacatacaga atcatattcc gcttgttttt agtgcggtac tcggcgtaat
     7081 gttcaatcat gtttgaaagt tgaagcacat atgctgcgta cttatcgtcg ttcgaggcgt
     7141 acagttgcca accgagcagg cgacagttct tttcattcag ggttttcaca atggaagagg
     7201 gagcattctc ttgctgttct cccgtttcac cgactgatat aatcagatta accgcttcag
     7261 gtgtattgcc gataagttcc aatgcacttc tcattgcctt ccatgcaggc aaaggagtat
     7321 tttctgtgtc agctacatgt gacgccattt tcaccaatcg gtctataagt atctcgtaat
     7381 cagcagtcag tggaattgtc tccatgcccc ggtgggtagc taccgccgct ccgaattgat
     7441 aggagaacga ttcaccggaa cctgtaaaaa acggtcccag attctgtatg gcattcaaca
     7501 gcattgggta ttgttccggt agccgggaag actgttccat tgcgaacagg atatttattc
     7561 gcttcaattc
//
