LOCUS       plasmid                 6557 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     mobile_element  795..1019
                     /note="pLannotate"
                     /label="Tn7R"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     mobile_element  3712..3877
                     /note="pLannotate"
                     /label="Tn7L"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     polyA_signal    complement(3906..4040)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(2944..3048)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(6111..6202)
                     /note="pLannotate"
                     /label="polyhedrin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(2083..2943)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5367..6059)
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="97.1"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1324..1912)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             195..728
                     /note="pLannotate"
                     /label="GmR"
                     /database="snapgene"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(3074..3528)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(4191..5330)
                     /note="pLannotate"
                     /label="wc-2 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="71.7"
                     /fragment="True"
                     /other="CDS"
     promoter        join(6554..6557,1..25)
                     /note="pLannotate"
                     /label="Pc promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    1087..1138
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5332..5351)
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.2"
                     /fragment="True"
                     /other="CDS"
     CDS             join(6229..6557,1..19)
                     /note="pLannotate"
                     /label="YPR2_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="94.8"
                     /match_length="88.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4063..4146)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3533..3549)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt
       61 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
      121 atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
      181 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
      241 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
      301 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
      361 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
      421 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
      481 tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg
      541 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
      601 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
      661 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
      721 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
      781 tgtcacaacg ccgcgaatat agtctttacc atgcccttgg ccacgcccct ctttaatacg
      841 acgggcaatt tgcacttcag aaaatgaaga gtttgcttta gccataacaa aagtccagta
      901 tgctttttca cagcataact ggactgattt cagtttacaa ctattctgtc tagtttaaga
      961 ctttattgtc atagtttaga tctattttgt tcagtttaag actttattgt ccgcccacac
     1021 ccgcttacgc agggcatcca tttattactc aaccgtaacc gattttgcca ggttacgcgg
     1081 ctggtctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     1141 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1201 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1261 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1321 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1381 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1441 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1501 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1561 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1621 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1681 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1741 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1801 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1861 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1921 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1981 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2041 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2101 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2161 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2221 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2281 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2341 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2401 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2461 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2521 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2581 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2641 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2701 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2761 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2821 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2881 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2941 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3001 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3061 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3121 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3181 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaga
     3241 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3301 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3361 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3421 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3481 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     3541 tcaggctgca aataagcgtt gatattcagt caattacaaa cattaataac gaagagatga
     3601 cagaaaaatt ttcattctgt gacagagaaa aagtagccga agatgacggt ttgtcacatg
     3661 gagttggcag gatgtttgat taaaaacata acaggaagaa aaatgccccg ctgtgggcgg
     3721 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
     3781 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
     3841 acaaaatagt ttggaactag atttcactta tctggttcgg atctcctagg ctcaagcagt
     3901 gatcagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3961 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4021 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4081 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     4141 atgatcctct agtacttctc gacaagcttg gtaccgcatg cctcgagtta tcccatatga
     4201 tcgcccatgg gtgtatggat gtcgttgtga ccgcctatac ctccgccgtt attgttgttg
     4261 ttggcgttct tctttttctc tttctttgcc cagcgaagac cgcaggcatt gcatagtgtc
     4321 ttgggtccac tagggccttt tcgccattcg ggggaatcga gcgtaccgca gtcggtgcac
     4381 acgtattcct ctgcaacctt gatcttcttc ttcttctctc ccgtccgggg atctcggtct
     4441 aggggaatcg cgattcccgc atcaccctta atgagcgtcg ggctggcatt acccgttgtg
     4501 ataccatgac ttctttcacc ctcctggtac ttgagtcctg taagcatttc aatcgtgtct
     4561 gcgtgattgc cctcgtaacg caacatcttt tcgcgtatag aatccggccg gcttccggca
     4621 atgccttcca ggttctcccg cgtcagcgca atgttaagcg acgacgccgg ctggctgggt
     4681 ggtggcatca taacgtcagc ttgggcattc atgggaatgt agaagggcgt cattcccatc
     4741 tgggtcgcgg tatcgtcgga cggcgtaaca tcagatcgtc cctcctggct cattcgccat
     4801 gtcctatgcg actcttcctg ctcctcctgc tcctctctgc gcagctcggc aatcctgcgc
     4861 ttcagtcttt cgttttcaat cttgtgttcc agaaaggaat ccaacagacc cgcgttcttg
     4921 gtcgggtagg gtcgagccat catgaacacg gcctggcaga aaggcgactg gttctgtgga
     4981 ttgggtgcaa acttggcggc ggcgatgtgc gcatgaccaa cagtctcgaa aatggtccag
     5041 tttccatcct ttttgcggaa ccggtagaac agtcgcaatt gggatcccgt cgcaatggcc
     5101 tcgttcaact ccgcggtgaa gaccccgacg tcatccgggt ggatcaggtc tctgaggaac
     5161 aagtcaatga tttcgggcgg cttatagccg gtcaggggtt ctacgctggg cgaaacatgc
     5221 ttgatccgtc cgttggcatc cagaatgtgt tcccagtcct ggagctcttc gacaaccttg
     5281 gcgggccaat tgcggcgctt ggtgaattcg gtcagggtgc ttccgccgcc tccctgaaaa
     5341 tacaggtttt cgtggtgatg gtggtggtgg gtgatgccag cggctgtcac gaattccagc
     5401 aggaccatgt ggtctctctt ctcgttaggg tccttagaca gagcggactg tgtgctcagg
     5461 tagtggttgt caggcagcag cacggggccg tcgccgatag gggtgttctg ctggtagtga
     5521 tcggccagct gcacgctgcc atcttccaca ttgtgtctga tcttgaagtt ggccttgata
     5581 ccattcttct gcttgtcggc tgtgatgtac acgttgtggc tgttgaagtt gtattccagc
     5641 ttgtggccca ggatgttgcc atcttccttg aagtcgatgc ccttcagctc gattctgttc
     5701 accagtgtat ctccctcgaa cttcacctcg gctctggtct tataggtgcc atcgtcttta
     5761 aaagagatgg tccgctcctg cacgtagccc tcaggcatgg cggatttgaa gaaatcatgc
     5821 cgcttcatgt ggtcggggta tctgctaaaa cattgcacgc cgtatgtcag ggttgtgacc
     5881 agggtgggcc aaggcacagg cagtttgccg gtggtgcaga taaatttcag ggtcagcttt
     5941 ccgttggtgg cgtcgccttc gccctcgccc cgcacgctga acttgtgtcc attcacgtcg
     6001 ccgtcgagct caaccagaat tggcaccact ccggtgaaca gttcctcgcc cttgctcatt
     6061 tcggaccggg atccgcgccc gatggtggga cggtatgaat aatccggaat atttataggt
     6121 ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc gagatggtta
     6181 tcattttaat tatctccatg atctattaat attccggagt atacgtagcc aaccactaga
     6241 actatagcta gagtcctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6301 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6361 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6421 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6481 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6541 ggcacgaacc cagttga
//
