LOCUS       plasmid                 6557 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     mobile_element  complement(6198..6422)
                     /note="pLannotate"
                     /label="Tn7R"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     mobile_element  complement(3340..3505)
                     /note="pLannotate"
                     /label="Tn7L"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     polyA_signal    3177..3311
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4169..4273
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1015..1106
                     /note="pLannotate"
                     /label="polyhedrin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4274..5134
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2322..3011
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="96.6"
                     /fragment="False"
                     /other="CDS"
     rep_origin      5305..5893
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(join(6489..6557,1..465))
                     /note="pLannotate"
                     /label="GmR"
                     /database="snapgene"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3689..4143
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1161..2300
                     /note="pLannotate"
                     /label="wc-2 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="71.7"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(635..663)
                     /note="pLannotate"
                     /label="Pc promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(6079..6130)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2301..2320
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(641..988)
                     /note="pLannotate"
                     /label="YPR2_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="94.8"
                     /match_length="88.5"
                     /fragment="True"
                     /other="CDS"
     CDS             3071..3154
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             3668..3684
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
       61 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
      121 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      181 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      241 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      301 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      361 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      421 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      481 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      541 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      601 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
      661 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
      721 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
      781 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
      841 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
      901 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
      961 ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg
     1021 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1081 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1141 gcggatcccg gtccgaaatg ggcggcggaa gcaccctgac cgaattcacc aagcgccgca
     1201 attggcccgc caaggttgtc gaagagctcc aggactggga acacattctg gatgccaacg
     1261 gacggatcaa gcatgtttcg cccagcgtag aacccctgac cggctataag ccgcccgaaa
     1321 tcattgactt gttcctcaga gacctgatcc acccggatga cgtcggggtc ttcaccgcgg
     1381 agttgaacga ggccattgcg acgggatccc aattgcgact gttctaccgg ttccggaaaa
     1441 aggatggaaa ctggaccatt ttcgagactg ttggtcatgc gcacatcgcc gccgccaagt
     1501 ttgcacccaa tccacagaac cagtcgcctt tctgccaggc cgtgttcatg atggctcgac
     1561 cctacccgac caagaacgcg ggtctgttgg attcctttct ggaacacaag attgaaaacg
     1621 aaagactgaa gcgcaggatt gccgagctgc gcagagagga gcaggaggag caggaagagt
     1681 cgcataggac atggcgaatg agccaggagg gacgatctga tgttacgccg tccgacgata
     1741 ccgcgaccca gatgggaatg acgcccttct acattcccat gaatgcccaa gctgacgtta
     1801 tgatgccacc acccagccag ccggcgtcgt cgcttaacat tgcgctgacg cgggagaacc
     1861 tggaaggcat tgccggaagc cggccggatt ctatacgcga aaagatgttg cgttacgagg
     1921 gcaatcacgc agacacgatt gaaatgctta caggactcaa gtaccaggag ggtgaaagaa
     1981 gtcatggtat cacaacgggt aatgccagcc cgacgctcat taagggtgat gcgggaatcg
     2041 cgattcccct agaccgagat ccccggacgg gagagaagaa gaagaagatc aaggttgcag
     2101 aggaatacgt gtgcaccgac tgcggtacgc tcgattcccc cgaatggcga aaaggcccta
     2161 gtggacccaa gacactatgc aatgcctgcg gtcttcgctg ggcaaagaaa gagaaaaaga
     2221 agaacgccaa caacaacaat aacggcggag gtataggcgg tcacaacgac atccatacac
     2281 ccatgggcga tcatatggga gaaaacctgt attttcaggg aagcaagggc gaggaactgt
     2341 tcaccggagt ggtgccaatt ctggttgagc tcgacggcga cgtgaatgga cacaagttca
     2401 gcgtgcgggg cgagggcgaa ggcgacgcca ccaacggaaa gctgaccctg aaatttatct
     2461 gcaccaccgg caaactgcct gtgccttggc ccaccctggt cacaaccctg acatacggcg
     2521 tgcaatgttt tagcagatac cccgaccaca tgaagcggca tgatttcttc aaatccgcca
     2581 tgcctgaggg ctacgtgcag gagcggacca tctcttttaa agacgatggc acctataaga
     2641 ccagagccga ggtgaagttc gagggagata cactggtgaa cagaatcgag ctgaagggca
     2701 tcgacttcaa ggaagatggc aacatcctgg gccacaagct ggaatacaac ttcaacagcc
     2761 acaacgtgta catcacagcc gacaagcaga agaatggtat caaggccaac ttcaagatca
     2821 gacacaatgt ggaagatggc agcgtgcagc tggccgatca ctaccagcag aacaccccta
     2881 tcggcgacgg ccccgtgctg ctgcctgaca accactacct gagcacacag tccgctctgt
     2941 ctaaggaccc taacgagaag agagaccaca tggtcctgct ggaattcgtg acagccgctg
     3001 gcatcaccca ccaccaccat caccactaac tcgaggcatg cggtaccaag cttgtcgaga
     3061 agtactagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3121 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3301 atgtctggat ctgatcactg cttgagccta ggagatccga accagataag tgaaatctag
     3361 ttccaaacta ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc
     3421 catctatttt gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag
     3481 ttcccaacta ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttttaatca
     3541 aacatcctgc caactccatg tgacaaaccg tcatcttcgg ctactttttc tctgtcacag
     3601 aatgaaaatt tttctgtcat ctcttcgtta ttaatgtttg taattgactg aatatcaacg
     3661 cttatttgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3961 acgttggagt ccacgttctt aatagtggac tcttgttcca aactggaaca acactcaacc
     4021 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4081 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     4141 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4501 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6001 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6061 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     6121 acaccgcata gaccagccgc gtaacctggc aaaatcggtt acggttgagt aataaatgga
     6181 tgccctgcgt aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta
     6241 aactatgaca ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt
     6301 atgctgtgaa aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga
     6361 agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta aagactatat
     6421 tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga
     6481 acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg
     6541 cccaactttg tatagag
//
