LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1433..2293)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(80..459)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(join(7133..7257,1..79))
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   complement(3210..3350)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(3863..3974)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(2294..2398)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(674..1262)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(2680..3135)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(7059..7085)
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3489..3729)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3154..3205
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4036..4058)
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2630..2679
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2493..2528
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4066..4088)
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(574..601)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   complement(518..545)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
       61 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
      121 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
      181 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
      241 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
      301 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
      361 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
      421 taattgatta ctattaataa ctagtcaata atcaatgtca acgcctcgag tctagaggcc
      481 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
      541 ggccgggcga ccaaaggtcg cccgacgccc gggcggcctc agtgagcgag cgagcgcgca
      601 gctgcctgca ggacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1381 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1561 caatgatacc gcggcttcca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1741 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2041 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2401 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     2461 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2521 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2581 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2641 atgcggcatc agagcagatt gtactgagag tgcaccataa aattgtaaac gttaatattt
     2701 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa tagaccgaaa
     2761 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agagttgagt gttgttccag
     2821 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2881 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
     2941 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3001 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaagg
     3061 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3121 cgctacaggg cgcgtactat ggttgctttg acgtatgcgg tgtgaaatac cgcacagatg
     3181 cgtaaggaga aaataccgca tcaggcgccc ctgcaggcag ctgcgcgctc gctcgctcac
     3241 tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag
     3301 cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct gcggccgcaa
     3361 acggcaaaaa tccttggact cataaggtgg ctttgatgct ccctccgaag agggagcttt
     3421 acccactacg acatagtcgc tggtgatggc tctcaaagta atcagataaa agcgccagtc
     3481 ggtgtttcgt cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac
     3541 ggtaagcata tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa
     3601 ttattacttt ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat
     3661 tccaattatc tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa
     3721 taggccctcg cggccagact aggcggccgt accaaaaaag ggtacctccc cagcatgcct
     3781 gctattctct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     3841 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     3901 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     3961 actagaaggc acagtcgagg ctgatcagcg agctctagga attcttaggc gtagtcaggt
     4021 acgtcgtaag gataggcgta atcgggtacg tcgtatggat aagcgtagtc aggcacatcg
     4081 tatgggtaac tgccgacctt ccgcttcttc tttggcaatg cattcacggc gggcttccag
     4141 acttctttca gcttttttct gtagaagctc tgccaggttt ccacaaaggc cctcttgtcg
     4201 gtgttgccgg tggtcttgtt ggtctggtac ttcttgtact cttggcttct caggaacagc
     4261 cagcttctgg caatgttcag ggcagcctgt tcatcggcgt gtgtctcgaa gccgcagttc
     4321 aggcacacga acttctcttg cacaggtctg tggctaaacc tcttcttcag caggctcaga
     4381 gcctcgcctg atctgccctt tgtccagctg ctgatgtcgt tgttcacgct ctcttcgctc
     4441 agtctgtcca gttccacgct cagatccttc acgacgttct gcctcttgta cctgttgtag
     4501 taggtgatct ggccctcaac cttcagctct ttgccgttga tggtggtcat ccagccggtg
     4561 gcggttttct tgagcttttc cagcactctg tcgtagtcgg cgctggtgat ggtgaagccg
     4621 caattgctgc aggtgcggga ggtatactgg gccagtgttt tggacaggta ggtcttgctg
     4681 ggcagtccct cgtaggccag tttagctgtg agccaatctt ccattctggt gtactgcctc
     4741 tcggccataa aggttctctt gccctgccgg ccgaagcctc tgctcagatt ctcgaagatc
     4801 agcatggcgt cctgtgtcac ggcgtagtac agcagatctc tggcggtgtt tcggaccatg
     4861 tcgtcggcca gattcttggc cttgctggcg tacttcctag agtatccgcc ggctcttctc
     4921 tgttccacct ctttggcggc ctggattgtc ctctgcttct ctttgtagct ctcgccgatt
     4981 ctcaggatgt gtgtagggtt gcccagggag tccttgaatc tgctcagtgg gcatccttca
     5041 gggtctgtca gggcgatcac agcagggata ttctcgcccc ggtcgatgcc gatcaggttc
     5101 atgggcttga tgttgctgct gtccagcacc tctcttctct cgaaggtcag ggccacaaac
     5161 agagcaggct cgtcctgtct ggttctcctg ttgtacaggg ttttctcgat cactctgcca
     5221 ttggccaggc gcagagagcc ggtttccagg ctcagcagat cgttccagat gaactctctg
     5281 ccctgtctct tgccgaaggc cagaggcagg ataatcaggt tggggtcgtc gaagttgaag
     5341 ttcacttcca tgggcacgat ctcgccggac tttttgttga tcacggtgta gaatctgtta
     5401 gcctcgaagg cctcgggttt gatcttcttg aaccgcagct tgccgccttt gaagtaattg
     5461 atgatcaggt acaggttcag tttcttgacg ccgtctttct gccaaatgaa ggcgcagttg
     5521 tactgcttgc tgaagccgct gatgtccagg atgctgttct cggcctcaat ggcgaagggc
     5581 ttgcctctca gatcgccgta ccacttctgc agcttcagct cgcatctgca gaactcgtcc
     5641 ttgtcggcct ctttcaggcc ctcgatcaca aagctggcct tggctctcag ccaatcggtc
     5701 agagcggctt tagattgggc gtcctcgctc cttctttcct cttccagctt aatgtgcttg
     5761 ctcaggcctt ccaccttctt gtcgattctc tcccaggcct catcgtacac tttgccccag
     5821 tcctcgccgt gcttcttttc caggtgcagc agcaggtcgc cgaactggta tctggcgaac
     5881 ttcttgccct tcttccggtc ctcttcgctg ctcaggtaag gcagcagggc ttcttgtctc
     5941 ttgtagccgg ccaggttctg ccagaaaacc ttgccatcct ctttcttctc gttgatcagc
     6001 ttcttcacgt tgcagaccat gtcccaccaa tccacttcat tggcctgtct ttccaccaga
     6061 gggaagctag ggaagccctt cagtctctgc agtggcttgg cttcgtccct gccgatcttc
     6121 agcttctgcc acagattcag gttgacccaa atcacgatct gggccaccac gttgttgtag
     6181 gcctcgatgc cctctttggt gtgaggctgt ggaggcaggg tgatcttagg aaaggccagg
     6241 ccgttagcgc tggcgatatc cttgaggttg gccagtcttt tctcgttctt cttgatcact
     6301 ttctggtgtt ccaggatgat gtcctggtac ttggtcagga aggatgccac ggctcccata
     6361 caggcgtcgc tcagggcttt tcccacaggt ccagaggcac agctattgcc gccgatctgt
     6421 tccaggggct tcacagggtg attgctctcc ctggtcacgt ggatgctgta gaagtccagg
     6481 gctctctgtc cgaacttgcc caggctgtat gtgaccagct catcgttggc ctcgggcttg
     6541 tgaggagaca gcaggatcag cctctcgtgc tcggacacgt tgcatctgcc gaagtagttg
     6601 gtgtggggct tgcccttgtc gttcacttgt tccagcttgt acacgtacag aggctggcaa
     6661 cactggctgc aggcaaagcc gctagaggtc agtctctcgt tgccgtcctt cacggggatc
     6721 agctttctct ggtcgatgtt cttaggagcg ggctgagcaa ctctgctcat caggcccacg
     6781 gggtctttct ggaactcttc ccagtacacg tgcaggatgg ctttcttcat ctcggtgtag
     6841 tcggtcagca gcttgttcag gttggccctg ctggtgttgc tgataggctg agggatgttc
     6901 tcgggcttct ttctcaggtt ttccagccgc tctctcaggt caggggtcat cactctgacg
     6961 agcagggttt tcatggggcc gcgcttgccg gccttctttg tgttgctgtc cttgaccagt
     7021 ctccttctga tcttgttgat tctcttgatc tcttgcatgt ccaacttgac cctcttggca
     7081 gcaggggatc cggacatggt ggcagcgctc tagaaccggt agttagccag agagctctgc
     7141 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     7201 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgt
//
