LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             5499..6359
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        76..455
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        456..659
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   4442..4582
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    3818..3929
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5394..5498
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      6530..7118
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      4657..5112
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             707..733
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4063..4303
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   complement(1..52)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="repeat_region"
     misc_feature    complement(4587..4638)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3734..3756
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5113..5162)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5264..5299)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             3704..3726
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   7191..7218
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggcctc
       61 tagactcgag gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      421 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      481 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      541 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      601 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      661 tctggctaac taccggttct agagcgctgc caccatgtcc ggatcccctg ctgccaagag
      721 ggtcaagttg gacatgcaag agatcaagag aatcaacaag atcagaagga gactggtcaa
      781 ggacagcaac acaaagaagg ccggcaagcg cggccccatg aaaaccctgc tcgtcagagt
      841 gatgacccct gacctgagag agcggctgga aaacctgaga aagaagcccg agaacatccc
      901 tcagcctatc agcaacacca gcagggccaa cctgaacaag ctgctgaccg actacaccga
      961 gatgaagaaa gccatcctgc acgtgtactg ggaagagttc cagaaagacc ccgtgggcct
     1021 gatgagcaga gttgctcagc ccgctcctaa gaacatcgac cagagaaagc tgatccccgt
     1081 gaaggacggc aacgagagac tgacctctag cggctttgcc tgcagccagt gttgccagcc
     1141 tctgtacgtg tacaagctgg aacaagtgaa cgacaagggc aagccccaca ccaactactt
     1201 cggcagatgc aacgtgtccg agcacgagag gctgatcctg ctgtctcctc acaagcccga
     1261 ggccaacgat gagctggtca catacagcct gggcaagttc ggacagagag ccctggactt
     1321 ctacagcatc cacgtgacca gggagagcaa tcaccctgtg aagcccctgg aacagatcgg
     1381 cggcaatagc tgtgcctctg gacctgtggg aaaagccctg agcgacgcct gtatgggagc
     1441 cgtggcatcc ttcctgacca agtaccagga catcatcctg gaacaccaga aagtgatcaa
     1501 gaagaacgag aaaagactgg ccaacctcaa ggatatcgcc agcgctaacg gcctggcctt
     1561 tcctaagatc accctgcctc cacagcctca caccaaagag ggcatcgagg cctacaacaa
     1621 cgtggtggcc cagatcgtga tttgggtcaa cctgaatctg tggcagaagc tgaagatcgg
     1681 cagggacgaa gccaagccac tgcagagact gaagggcttc cctagcttcc ctctggtgga
     1741 aagacaggcc aatgaagtgg attggtggga catggtctgc aacgtgaaga agctgatcaa
     1801 cgagaagaaa gaggatggca aggttttctg gcagaacctg gccggctaca agagacaaga
     1861 agccctgctg ccttacctga gcagcgaaga ggaccggaag aagggcaaga agttcgccag
     1921 ataccagttc ggcgacctgc tgctgcacct ggaaaagaag cacggcgagg actggggcaa
     1981 agtgtacgat gaggcctggg agagaatcga caagaaggtg gaaggcctga gcaagcacat
     2041 taagctggaa gaggaaagaa ggagcgagga cgcccaatct aaagccgctc tgaccgattg
     2101 gctgagagcc aaggccagct ttgtgatcga gggcctgaaa gaggccgaca aggacgagtt
     2161 ctgcagatgc gagctgaagc tgcagaagtg gtacggcgat ctgagaggca agcccttcgc
     2221 cattgaggcc gagaacagca tcctggacat cagcggcttc agcaagcagt acaactgcgc
     2281 cttcatttgg cagaaagacg gcgtcaagaa actgaacctg tacctgatca tcaattactt
     2341 caaaggcggc aagctgcggt tcaagaagat caaacccgag gccttcgagg ctaacagatt
     2401 ctacaccgtg atcaacaaaa agtccggcga gatcgtgccc atggaagtga acttcaactt
     2461 cgacgacccc aacctgatta tcctgcctct ggccttcggc aagagacagg gcagagagtt
     2521 catctggaac gatctgctga gcctggaaac cggctctctg cgcctggcca atggcagagt
     2581 gatcgagaaa accctgtaca acaggagaac cagacaggac gagcctgctc tgtttgtggc
     2641 cctgaccttc gagagaagag aggtgctgga cagcagcaac atcaagccca tgaacctgat
     2701 cggcatcgac cggggcgaga atatccctgc tgtgatcgcc ctgacagacc ctgaaggatg
     2761 cccactgagc agattcaagg actccctggg caaccctaca cacatcctga gaatcggcga
     2821 gagctacaaa gagaagcaga ggacaatcca ggccgccaaa gaggtggaac agagaagagc
     2881 cggcggatac tctaggaagt acgccagcaa ggccaagaat ctggccgacg acatggtccg
     2941 aaacaccgcc agagatctgc tgtactacgc cgtgacacag gacgccatgc tgatcttcga
     3001 gaatctgagc agaggcttcg gccggcaggg caagagaacc tttatggccg agaggcagta
     3061 caccagaatg gaagattggc tcacagctaa actggcctac gagggactgc ccagcaagac
     3121 ctacctgtcc aaaacactgg cccagtatac ctcccgcacc tgcagcaatt gcggcttcac
     3181 catcaccagc gccgactacg acagagtgct ggaaaagctc aagaaaaccg ccaccggctg
     3241 gatgaccacc atcaacggca aagagctgaa ggttgagggc cagatcacct actacaacag
     3301 gtacaagagg cagaacgtcg tgaaggatct gagcgtggaa ctggacagac tgagcgaaga
     3361 gagcgtgaac aacgacatca gcagctggac aaagggcaga tcaggcgagg ctctgagcct
     3421 gctgaagaag aggtttagcc acagacctgt gcaagagaag ttcgtgtgcc tgaactgcgg
     3481 cttcgagaca cacgccgatg aacaggctgc cctgaacatt gccagaagct ggctgttcct
     3541 gagaagccaa gagtacaaga agtaccagac caacaagacc accggcaaca ccgacaagag
     3601 ggcctttgtg gaaacctggc agagcttcta cagaaaaaag ctgaaagaag tctggaagcc
     3661 cgccgtgaat gcattgccaa agaagaagcg gaaggtcggc agttacccat acgatgtgcc
     3721 tgactacgct tatccatacg acgtacccga ttacgcctat ccttacgacg tacctgacta
     3781 cgcctaagaa ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     3841 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3901 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3961 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct
     4021 ggggaggtac ccttttttgg tacggccgcc tagtctggcc gcgagggcct atttcccatg
     4081 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     4141 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     4201 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     4261 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgactggcgc
     4321 ttttatctga ttactttgag agccatcacc agcgactatg tcgtagtggg taaagctccc
     4381 tcttcggagg gagcatcaaa gccaccttat gagtccaagg atttttgccg tttgcggccg
     4441 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     4501 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4561 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4621 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4681 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4741 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4801 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4861 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4921 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4981 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     5041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5101 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     5161 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     5221 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     5281 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5341 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     5401 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5461 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5521 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5581 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5641 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5701 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5761 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5821 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5881 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5941 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6001 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6061 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6121 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6181 ttattgctga taaatctgga gccggtgagc gtggaagccg cggtatcatt gcagcactgg
     6241 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6301 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6361 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6421 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6481 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6541 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6601 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6661 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6721 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6781 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6841 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6901 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6961 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7021 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7081 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7141 tacggttcct ggccttttgc tggccttttg ctcacatgtc ctgcaggcag ctgcgcgctc
     7201 gctcgctcac tgaggccgcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtg
//
