LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2392..3252
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        4226..4605
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        4606..4809
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   1335..1475
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    711..822
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        2287..2391
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      3423..4011
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      1550..2005
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             4857..4883
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        956..1196
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(1480..1531)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             627..649
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2006..2055)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2157..2192)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             597..619
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   4084..4111
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   4140..4167
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 tgcccagcaa gacctacctg tccaaaacac tggcccagta tacctcccgc acctgcagca
       61 attgcggctt caccatcacc agcgccgact acgacagagt gctggaaaag ctcaagaaaa
      121 ccgccaccgg ctggatgacc accatcaacg gcaaagagct gaaggttgag ggccagatca
      181 cctactacaa caggtacaag aggcagaacg tcgtgaagga tctgagcgtg gaactggaca
      241 gactgagcga agagagcgtg aacaacgaca tcagcagctg gacaaagggc agatcaggcg
      301 aggctctgag cctgctgaag aagaggttta gccacagacc tgtgcaagag aagttcgtgt
      361 gcctgaactg cggcttcgag acacacgccg atgaacaggc tgccctgaac attgccagaa
      421 gctggctgtt cctgagaagc caagagtaca agaagtacca gaccaacaag accaccggca
      481 acaccgacaa gagggccttt gtggaaacct ggcagagctt ctacagaaaa aagctgaaag
      541 aagtctggaa gcccgccgtg aatgcattgc caaagaagaa gcggaaggtc ggcagttacc
      601 catacgatgt gcctgactac gcttatccat acgacgtacc cgattacgcc tatccttacg
      661 acgtacctga ctacgcctaa gaattcctag agctcgctga tcagcctcga ctgtgccttc
      721 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
      781 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
      841 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa
      901 tagcaggcat gctggggagg tacccttttt tggtacggcc gcctagtctg gccgcgaggg
      961 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     1021 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     1081 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     1141 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     1201 caccgactgg cgcttttatc tgattacttt gagagccatc accagcgact atgtcgtagt
     1261 gggtaaagct ccctcttcgg agggagcatc aaagggataa taaggtttaa tacctttttg
     1321 ccgtttgcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     1381 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     1441 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     1501 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     1561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1621 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1741 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     1801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1861 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     1921 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1981 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     2041 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     2101 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     2161 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     2221 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2281 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2341 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2401 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2461 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2521 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2581 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2641 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2701 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2761 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2821 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2881 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2941 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3001 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3061 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc
     3121 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3181 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3241 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3301 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtcctgcagg
     4081 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc
     4141 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
     4201 ctgcggcctc tagactcgag gcgttgacat tgattattga ctagttatta atagtaatca
     4261 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4321 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4381 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4441 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4501 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4561 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     4621 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     4681 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     4741 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     4801 agcagagctc tctggctaac taccggttct agagcgctgc caccatgtcc ggatcccctg
     4861 ctgccaagag ggtcaagttg gacatgcaag agatcaagag aatcaacaag atcagaagga
     4921 gactggtcaa ggacagcaac acaaagaagg ccggcaagcg cggccccatg aaaaccctgc
     4981 tcgtcagagt gatgacccct gacctgagag agcggctgga aaacctgaga aagaagcccg
     5041 agaacatccc tcagcctatc agcaacacca gcagggccaa cctgaacaag ctgctgaccg
     5101 actacaccga gatgaagaaa gccatcctgc acgtgtactg ggaagagttc cagaaagacc
     5161 ccgtgggcct gatgagcaga gttgctcagc ccgctcctaa gaacatcgac cagagaaagc
     5221 tgatccccgt gaaggacggc aacgagagac tgacctctag cggctttgcc tgcagccagt
     5281 gttgccagcc tctgtacgtg tacaagctgg aacaagtgaa cgacaagggc aagccccaca
     5341 ccaactactt cggcagatgc aacgtgtccg agcacgagag gctgatcctg ctgtctcctc
     5401 acaagcccga ggccaacgat gagctggtca catacagcct gggcaagttc ggacagagag
     5461 ccctggactt ctacagcatc cacgtgacca gggagagcaa tcaccctgtg aagcccctgg
     5521 aacagatcgg cggcaatagc tgtgcctctg gacctgtggg aaaagccctg agcgacgcct
     5581 gtatgggagc cgtggcatcc ttcctgacca agtaccagga catcatcctg gaacaccaga
     5641 aagtgatcaa gaagaacgag aaaagactgg ccaacctcaa ggatatcgcc agcgctaacg
     5701 gcctggcctt tcctaagatc accctgcctc cacagcctca caccaaagag ggcatcgagg
     5761 cctacaacaa cgtggtggcc cagatcgtga tttgggtcaa cctgaatctg tggcagaagc
     5821 tgaagatcgg cagggacgaa gccaagccac tgcagagact gaagggcttc cctagcttcc
     5881 ctctggtgga aagacaggcc aatgaagtgg attggtggga catggtctgc aacgtgaaga
     5941 agctgatcaa cgagaagaaa gaggatggca aggttttctg gcagaacctg gccggctaca
     6001 agagacaaga agccctgctg ccttacctga gcagcgaaga ggaccggaag aagggcaaga
     6061 agttcgccag ataccagttc ggcgacctgc tgctgcacct ggaaaagaag cacggcgagg
     6121 actggggcaa agtgtacgat gaggcctggg agagaatcga caagaaggtg gaaggcctga
     6181 gcaagcacat taagctggaa gaggaaagaa ggagcgagga cgcccaatct aaagccgctc
     6241 tgaccgattg gctgagagcc aaggccagct ttgtgatcga gggcctgaaa gaggccgaca
     6301 aggacgagtt ctgcagatgc gagctgaagc tgcagaagtg gtacggcgat ctgagaggca
     6361 agcccttcgc cattgaggcc gagaacagca tcctggacat cagcggcttc agcaagcagt
     6421 acaactgcgc cttcatttgg cagaaagacg gcgtcaagaa actgaacctg tacctgatca
     6481 tcaattactt caaaggcggc aagctgcggt tcaagaagat caaacccgag gccttcgagg
     6541 ctaacagatt ctacaccgtg atcaacaaaa agtccggcga gatcgtgccc atggaagtga
     6601 acttcaactt cgacgacccc aacctgatta tcctgcctct ggccttcggc aagagacagg
     6661 gcagagagtt catctggaac gatctgctga gcctggaaac cggctctctg cgcctggcca
     6721 atggcagagt gatcgagaaa accctgtaca acaggagaac cagacaggac gagcctgctc
     6781 tgtttgtggc cctgaccttc gagagaagag aggtgctgga cagcagcaac atcaagccca
     6841 tgaacctgat cggcatcgac cggggcgaga atatccctgc tgtgatcgcc ctgacagacc
     6901 ctgaaggatg cccactgagc agattcaagg actccctggg caaccctaca cacatcctga
     6961 gaatcggcga gagctacaaa gagaagcaga ggacaatcca ggccgccaaa gaggtggaac
     7021 agagaagagc cggcggatac tctaggaagt acgccagcaa ggccaagaat ctggccgacg
     7081 acatggtccg aaacaccgcc agagatctgc tgtactacgc cgtgacacag gacgccatgc
     7141 tgatcttcga gaatctgagc agaggcttcg gccggcaggg caagagaacc tttatggccg
     7201 agaggcagta caccagaatg gaagattggc tcacagctaa actggcctac gagggac
//
