LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(6108..6968)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(4755..5134)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(4551..4754)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   complement(628..768)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(1281..1392)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(6969..7073)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(5349..5937)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(98..553)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(4477..4503)
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(907..1147)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    572..623
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1454..1476)
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    48..97
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             7168..7203
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1484..1506)
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(5249..5276)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   complement(5193..5220)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
       61 gcggcatcag agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg
      121 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata gaccgaaatc
      181 ggcaaaatcc cttataaatc aaaagaatag cccgagatag agttgagtgt tgttccagtt
      241 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
      301 tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg
      361 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
      421 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctaaggcg
      481 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
      541 ctacagggcg cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg
      601 taaggagaaa ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg
      661 aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg
      721 agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcaaac
      781 ggcaaaaagg tattaaacct tattatccct ttgatgctcc ctccgaagag ggagctttac
      841 ccactacgac atagtcgctg gtgatggctc tcaaagtaat cagataaaag cgccagtcgg
      901 tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg
      961 taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt
     1021 attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc
     1081 caattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata
     1141 ggccctcgcg gccagactag gcggccgtac caaaaaaggg tacctcccca gcatgcctgc
     1201 tattctcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     1261 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     1321 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     1381 tagaaggcac agtcgaggct gatcagcgag ctctaggaat tcttaggcgt agtcaggtac
     1441 gtcgtaagga taggcgtaat cgggtacgtc gtatggataa gcgtagtcag gcacatcgta
     1501 tgggtaactg ccgaccttcc gcttcttctt tggcaatgca ttcacggcgg gcttccagac
     1561 ttctttcagc ttttttctgt agaagctctg ccaggtttcc acaaaggccc tcttgtcggt
     1621 gttgccggtg gtcttgttgg tctggtactt cttgtactct tggcttctca ggaacagcca
     1681 gcttctggca atgttcaggg cagcctgttc atcggcgtgt gtctcgaagc cgcagttcag
     1741 gcacacgaac ttctcttgca caggtctgtg gctaaacctc ttcttcagca ggctcagagc
     1801 ctcgcctgat ctgccctttg tccagctgct gatgtcgttg ttcacgctct cttcgctcag
     1861 tctgtccagt tccacgctca gatccttcac gacgttctgc ctcttgtacc tgttgtagta
     1921 ggtgatctgg ccctcaacct tcagctcttt gccgttgatg gtggtcatcc agccggtggc
     1981 ggttttcttg agcttttcca gcactctgtc gtagtcggcg ctggtgatgg tgaagccgca
     2041 attgctgcag gtgcgggagg tatactgggc cagtgttttg gacaggtagg tcttgctggg
     2101 cagtccctcg taggccagtt tagctgtgag ccaatcttcc attctggtgt actgcctctc
     2161 ggccataaag gttctcttgc cctgccggcc gaagcctctg ctcagattct cgaagatcag
     2221 catggcgtcc tgtgtcacgg cgtagtacag cagatctctg gcggtgtttc ggaccatgtc
     2281 gtcggccaga ttcttggcct tgctggcgta cttcctagag tatccgccgg ctcttctctg
     2341 ttccacctct ttggcggcct ggattgtcct ctgcttctct ttgtagctct cgccgattct
     2401 caggatgtgt gtagggttgc ccagggagtc cttgaatctg ctcagtgggc atccttcagg
     2461 gtctgtcagg gcgatcacag cagggatatt ctcgccccgg tcgatgccga tcaggttcat
     2521 gggcttgatg ttgctgctgt ccagcacctc tcttctctcg aaggtcaggg ccacaaacag
     2581 agcaggctcg tcctgtctgg ttctcctgtt gtacagggtt ttctcgatca ctctgccatt
     2641 ggccaggcgc agagagccgg tttccaggct cagcagatcg ttccagatga actctctgcc
     2701 ctgtctcttg ccgaaggcca gaggcaggat aatcaggttg gggtcgtcga agttgaagtt
     2761 cacttccatg ggcacgatct cgccggactt tttgttgatc acggtgtaga atctgttagc
     2821 ctcgaaggcc tcgggtttga tcttcttgaa ccgcagcttg ccgcctttga agtaattgat
     2881 gatcaggtac aggttcagtt tcttgacgcc gtctttctgc caaatgaagg cgcagttgta
     2941 ctgcttgctg aagccgctga tgtccaggat gctgttctcg gcctcaatgg cgaagggctt
     3001 gcctctcaga tcgccgtacc acttctgcag cttcagctcg catctgcaga actcgtcctt
     3061 gtcggcctct ttcaggccct cgatcacaaa gctggccttg gctctcagcc aatcggtcag
     3121 agcggcttta gattgggcgt cctcgctcct tctttcctct tccagcttaa tgtgcttgct
     3181 caggccttcc accttcttgt cgattctctc ccaggcctca tcgtacactt tgccccagtc
     3241 ctcgccgtgc ttcttttcca ggtgcagcag caggtcgccg aactggtatc tggcgaactt
     3301 cttgcccttc ttccggtcct cttcgctgct caggtaaggc agcagggctt cttgtctctt
     3361 gtagccggcc aggttctgcc agaaaacctt gccatcctct ttcttctcgt tgatcagctt
     3421 cttcacgttg cagaccatgt cccaccaatc cacttcattg gcctgtcttt ccaccagagg
     3481 gaagctaggg aagcccttca gtctctgcag tggcttggct tcgtccctgc cgatcttcag
     3541 cttctgccac agattcaggt tgacccaaat cacgatctgg gccaccacgt tgttgtaggc
     3601 ctcgatgccc tctttggtgt gaggctgtgg aggcagggtg atcttaggaa aggccaggcc
     3661 gttagcgctg gcgatatcct tgaggttggc cagtcttttc tcgttcttct tgatcacttt
     3721 ctggtgttcc aggatgatgt cctggtactt ggtcaggaag gatgccacgg ctcccataca
     3781 ggcgtcgctc agggcttttc ccacaggtcc agaggcacag ctattgccgc cgatctgttc
     3841 caggggcttc acagggtgat tgctctccct ggtcacgtgg atgctgtaga agtccagggc
     3901 tctctgtccg aacttgccca ggctgtatgt gaccagctca tcgttggcct cgggcttgtg
     3961 aggagacagc aggatcagcc tctcgtgctc ggacacgttg catctgccga agtagttggt
     4021 gtggggcttg cccttgtcgt tcacttgttc cagcttgtac acgtacagag gctggcaaca
     4081 ctggctgcag gcaaagccgc tagaggtcag tctctcgttg ccgtccttca cggggatcag
     4141 ctttctctgg tcgatgttct taggagcggg ctgagcaact ctgctcatca ggcccacggg
     4201 gtctttctgg aactcttccc agtacacgtg caggatggct ttcttcatct cggtgtagtc
     4261 ggtcagcagc ttgttcaggt tggccctgct ggtgttgctg ataggctgag ggatgttctc
     4321 gggcttcttt ctcaggtttt ccagccgctc tctcaggtca ggggtcatca ctctgacgag
     4381 cagggttttc atggggccgc gcttgccggc cttctttgtg ttgctgtcct tgaccagtct
     4441 ccttctgatc ttgttgattc tcttgatctc ttgcatgtcc aacttgaccc tcttggcagc
     4501 aggggatccg gacatggtgg cagcgctcta gaaccggtag ttagccagag agctctgctt
     4561 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     4621 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     4681 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     4741 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     4801 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     4861 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     4921 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     4981 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     5041 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     5101 gattactatt aataactagt caataatcaa tgtcaacgcc tcgagtctag aggccgcagg
     5161 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     5221 ggcgaccaaa ggtcgcccga cgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
     5281 ctgcaggaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5341 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5401 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5461 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5521 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5581 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5641 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5701 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5761 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5821 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5881 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5941 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6001 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6061 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6121 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6181 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6241 ataccgcggc ttccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6301 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6361 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6421 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6481 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6541 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6601 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6661 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6721 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6781 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6841 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6901 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6961 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7021 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7081 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     7141 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7201 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagc
//
