LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             4664..5524
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        6498..6877
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        6878..7081
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   3607..3747
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    2983..3094
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4559..4663
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      5695..6283
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      3822..4277
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             7129..7155
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        3228..3468
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3752..3803)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2899..2921
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(4278..4327)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4429..4464)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             2869..2891
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   6356..6383
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   6412..6439
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 agagtgatga cccctgacct gagagagcgg ctggaaaacc tgagaaagaa gcccgagaac
       61 atccctcagc ctatcagcaa caccagcagg gccaacctga acaagctgct gaccgactac
      121 accgagatga agaaagccat cctgcacgtg tactgggaag agttccagaa agaccccgtg
      181 ggcctgatga gcagagttgc tcagcccgct cctaagaaca tcgaccagag aaagctgatc
      241 cccgtgaagg acggcaacga gagactgacc tctagcggct ttgcctgcag ccagtgttgc
      301 cagcctctgt acgtgtacaa gctggaacaa gtgaacgaca agggcaagcc ccacaccaac
      361 tacttcggca gatgcaacgt gtccgagcac gagaggctga tcctgctgtc tcctcacaag
      421 cccgaggcca acgatgagct ggtcacatac agcctgggca agttcggaca gagagccctg
      481 gacttctaca gcatccacgt gaccagggag agcaatcacc ctgtgaagcc cctggaacag
      541 atcggcggca atagctgtgc ctctggacct gtgggaaaag ccctgagcga cgcctgtatg
      601 ggagccgtgg catccttcct gaccaagtac caggacatca tcctggaaca ccagaaagtg
      661 atcaagaaga acgagaaaag actggccaac ctcaaggata tcgccagcgc taacggcctg
      721 gcctttccta agatcaccct gcctccacag cctcacacca aagagggcat cgaggcctac
      781 aacaacgtgg tggcccagat cgtgatttgg gtcaacctga atctgtggca gaagctgaag
      841 atcggcaggg acgaagccaa gccactgcag agactgaagg gcttccctag cttccctctg
      901 gtggaaagac aggccaatga agtggattgg tgggacatgg tctgcaacgt gaagaagctg
      961 atcaacgaga agaaagagga tggcaaggtt ttctggcaga acctggccgg ctacaagaga
     1021 caagaagccc tgctgcctta cctgagcagc gaagaggacc ggaagaaggg caagaagttc
     1081 gccagatacc agttcggcga cctgctgctg cacctggaaa agaagcacgg cgaggactgg
     1141 ggcaaagtgt acgatgaggc ctgggagaga atcgacaaga aggtggaagg cctgagcaag
     1201 cacattaagc tggaagagga aagaaggagc gaggacgccc aatctaaagc cgctctgacc
     1261 gattggctga gagccaaggc cagctttgtg atcgagggcc tgaaagaggc cgacaaggac
     1321 gagttctgca gatgcgagct gaagctgcag aagtggtacg gcgatctgag aggcaagccc
     1381 ttcgccattg aggccgagaa cagcatcctg gacatcagcg gcttcagcaa gcagtacaac
     1441 tgcgccttca tttggcagaa agacggcgtc aagaaactga acctgtacct gatcatcaat
     1501 tacttcaaag gcggcaagct gcggttcaag aagatcaaac ccgaggcctt cgaggctaac
     1561 agattctaca ccgtgatcaa caaaaagtcc ggcgagatcg tgcccatgga agtgaacttc
     1621 aacttcgacg accccaacct gattatcctg cctctggcct tcggcaagag acagggcaga
     1681 gagttcatct ggaacgatct gctgagcctg gaaaccggct ctctgcgcct ggccaatggc
     1741 agagtgatcg agaaaaccct gtacaacagg agaaccagac aggacgagcc tgctctgttt
     1801 gtggccctga ccttcgagag aagagaggtg ctggacagca gcaacatcaa gcccatgaac
     1861 ctgatcggca tcgaccgggg cgagaatatc cctgctgtga tcgccctgac agaccctgaa
     1921 ggatgcccac tgagcagatt caaggactcc ctgggcaacc ctacacacat cctgagaatc
     1981 ggcgagagct acaaagagaa gcagaggaca atccaggccg ccaaagaggt ggaacagaga
     2041 agagccggcg gatactctag gaagtacgcc agcaaggcca agaatctggc cgacgacatg
     2101 gtccgaaaca ccgccagaga tctgctgtac tacgccgtga cacaggacgc catgctgatc
     2161 ttcgagaatc tgagcagagg cttcggccgg cagggcaaga gaacctttat ggccgagagg
     2221 cagtacacca gaatggaaga ttggctcaca gctaaactgg cctacgaggg actgcccagc
     2281 aagacctacc tgtccaaaac actggcccag tatacctccc gcacctgcag caattgcggc
     2341 ttcaccatca ccagcgccga ctacgacaga gtgctggaaa agctcaagaa aaccgccacc
     2401 ggctggatga ccaccatcaa cggcaaagag ctgaaggttg agggccagat cacctactac
     2461 aacaggtaca agaggcagaa cgtcgtgaag gatctgagcg tggaactgga cagactgagc
     2521 gaagagagcg tgaacaacga catcagcagc tggacaaagg gcagatcagg cgaggctctg
     2581 agcctgctga agaagaggtt tagccacaga cctgtgcaag agaagttcgt gtgcctgaac
     2641 tgcggcttcg agacacacgc cgatgaacag gctgccctga acattgccag aagctggctg
     2701 ttcctgagaa gccaagagta caagaagtac cagaccaaca agaccaccgg caacaccgac
     2761 aagagggcct ttgtggaaac ctggcagagc ttctacagaa aaaagctgaa agaagtctgg
     2821 aagcccgccg tgaatgcatt gccaaagaag aagcggaagg tcggcagtta cccatacgat
     2881 gtgcctgact acgcttatcc atacgacgta cccgattacg cctatcctta cgacgtacct
     2941 gactacgcct aagaattcct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     3001 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3061 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3121 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc
     3181 atgctgggga ggtacccttt tttggtacgg ccgcctagtc tggccgcgag ggcctatttc
     3241 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     3301 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     3361 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     3421 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgact
     3481 ggcgctttta tctgattact ttgagagcca tcaccagcga ctatgtcgta gtgggtaaag
     3541 ctccctcttc ggagggagca tcaaagcgag attgagatct tctgcgtttt tgccgtttgc
     3601 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     3661 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     3721 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     3781 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     3841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     3901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4021 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4141 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     4201 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4261 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4321 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4381 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4441 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4501 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4561 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4621 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4681 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4741 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4801 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4861 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4921 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4981 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5041 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5101 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5161 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5221 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5281 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5341 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     5401 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5461 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5521 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5581 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5641 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5701 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5761 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5821 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5881 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5941 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6001 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6061 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6121 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6181 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6241 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6301 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtcctgca ggcagctgcg
     6361 cgctcgctcg ctcactgagg ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag
     6421 tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt tcctgcggcc
     6481 tctagactcg aggcgttgac attgattatt gactagttat taatagtaat caattacggg
     6541 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     6601 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     6661 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     6721 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     6781 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     6841 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     6901 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     6961 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     7021 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     7081 tctctggcta actaccggtt ctagagcgct gccaccatgt ccggatcccc tgctgccaag
     7141 agggtcaagt tggacatgca agagatcaag agaatcaaca agatcagaag gagactggtc
     7201 aaggacagca acacaaagaa ggccggcaag cgcggcccca tgaaaaccct gctcgtc
//
