LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             3065..3925
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        4899..5278
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        5279..5482
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   2008..2148
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    1384..1495
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        2960..3064
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      4096..4684
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      2223..2678
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             5530..5556
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        1629..1869
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(2153..2204)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1300..1322
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2679..2728)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2830..2865)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1270..1292
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   4757..4784
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   4813..4840
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 gtgcccatgg aagtgaactt caacttcgac gaccccaacc tgattatcct gcctctggcc
       61 ttcggcaaga gacagggcag agagttcatc tggaacgatc tgctgagcct ggaaaccggc
      121 tctctgcgcc tggccaatgg cagagtgatc gagaaaaccc tgtacaacag gagaaccaga
      181 caggacgagc ctgctctgtt tgtggccctg accttcgaga gaagagaggt gctggacagc
      241 agcaacatca agcccatgaa cctgatcggc atcgaccggg gcgagaatat ccctgctgtg
      301 atcgccctga cagaccctga aggatgccca ctgagcagat tcaaggactc cctgggcaac
      361 cctacacaca tcctgagaat cggcgagagc tacaaagaga agcagaggac aatccaggcc
      421 gccaaagagg tggaacagag aagagccggc ggatactcta ggaagtacgc cagcaaggcc
      481 aagaatctgg ccgacgacat ggtccgaaac accgccagag atctgctgta ctacgccgtg
      541 acacaggacg ccatgctgat cttcgagaat ctgagcagag gcttcggccg gcagggcaag
      601 agaaccttta tggccgagag gcagtacacc agaatggaag attggctcac agctaaactg
      661 gcctacgagg gactgcccag caagacctac ctgtccaaaa cactggccca gtatacctcc
      721 cgcacctgca gcaattgcgg cttcaccatc accagcgccg actacgacag agtgctggaa
      781 aagctcaaga aaaccgccac cggctggatg accaccatca acggcaaaga gctgaaggtt
      841 gagggccaga tcacctacta caacaggtac aagaggcaga acgtcgtgaa ggatctgagc
      901 gtggaactgg acagactgag cgaagagagc gtgaacaacg acatcagcag ctggacaaag
      961 ggcagatcag gcgaggctct gagcctgctg aagaagaggt ttagccacag acctgtgcaa
     1021 gagaagttcg tgtgcctgaa ctgcggcttc gagacacacg ccgatgaaca ggctgccctg
     1081 aacattgcca gaagctggct gttcctgaga agccaagagt acaagaagta ccagaccaac
     1141 aagaccaccg gcaacaccga caagagggcc tttgtggaaa cctggcagag cttctacaga
     1201 aaaaagctga aagaagtctg gaagcccgcc gtgaatgcat tgccaaagaa gaagcggaag
     1261 gtcggcagtt acccatacga tgtgcctgac tacgcttatc catacgacgt acccgattac
     1321 gcctatcctt acgacgtacc tgactacgcc taagaattcc tagagctcgc tgatcagcct
     1381 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1441 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1501 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1561 attgggaaga gaatagcagg catgctgggg aggtaccctt ttttggtacg gccgcctagt
     1621 ctggccgcga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     1681 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     1741 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     1801 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1861 ggaaaggacg aaacaccgac tggcgctttt atctgattac tttgagagcc atcaccagcg
     1921 actatgtcgt agtgggtaaa gctccctctt cggagggagc atcaaagcga gattgagatc
     1981 ttctgcgttt ttgccgtttg cggccgcagg aacccctagt gatggagttg gccactccct
     2041 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2101 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     2161 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     2221 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     2281 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     2341 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     2401 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     2461 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2521 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     2581 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     2641 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     2701 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2761 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2821 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3301 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3781 aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4741 catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga
     4801 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca
     4861 tcactagggg ttcctgcggc ctctagactc gaggcgttga cattgattat tgactagtta
     4921 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4981 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     5041 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     5101 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     5161 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     5221 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     5281 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     5341 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     5401 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     5461 ggaggtctat ataagcagag ctctctggct aactaccggt tctagagcgc tgccaccatg
     5521 tccggatccc ctgctgccaa gagggtcaag ttggacatgc aagagatcaa gagaatcaac
     5581 aagatcagaa ggagactggt caaggacagc aacacaaaga aggccggcaa gcgcggcccc
     5641 atgaaaaccc tgctcgtcag agtgatgacc cctgacctga gagagcggct ggaaaacctg
     5701 agaaagaagc ccgagaacat ccctcagcct atcagcaaca ccagcagggc caacctgaac
     5761 aagctgctga ccgactacac cgagatgaag aaagccatcc tgcacgtgta ctgggaagag
     5821 ttccagaaag accccgtggg cctgatgagc agagttgctc agcccgctcc taagaacatc
     5881 gaccagagaa agctgatccc cgtgaaggac ggcaacgaga gactgacctc tagcggcttt
     5941 gcctgcagcc agtgttgcca gcctctgtac gtgtacaagc tggaacaagt gaacgacaag
     6001 ggcaagcccc acaccaacta cttcggcaga tgcaacgtgt ccgagcacga gaggctgatc
     6061 ctgctgtctc ctcacaagcc cgaggccaac gatgagctgg tcacatacag cctgggcaag
     6121 ttcggacaga gagccctgga cttctacagc atccacgtga ccagggagag caatcaccct
     6181 gtgaagcccc tggaacagat cggcggcaat agctgtgcct ctggacctgt gggaaaagcc
     6241 ctgagcgacg cctgtatggg agccgtggca tccttcctga ccaagtacca ggacatcatc
     6301 ctggaacacc agaaagtgat caagaagaac gagaaaagac tggccaacct caaggatatc
     6361 gccagcgcta acggcctggc ctttcctaag atcaccctgc ctccacagcc tcacaccaaa
     6421 gagggcatcg aggcctacaa caacgtggtg gcccagatcg tgatttgggt caacctgaat
     6481 ctgtggcaga agctgaagat cggcagggac gaagccaagc cactgcagag actgaagggc
     6541 ttccctagct tccctctggt ggaaagacag gccaatgaag tggattggtg ggacatggtc
     6601 tgcaacgtga agaagctgat caacgagaag aaagaggatg gcaaggtttt ctggcagaac
     6661 ctggccggct acaagagaca agaagccctg ctgccttacc tgagcagcga agaggaccgg
     6721 aagaagggca agaagttcgc cagataccag ttcggcgacc tgctgctgca cctggaaaag
     6781 aagcacggcg aggactgggg caaagtgtac gatgaggcct gggagagaat cgacaagaag
     6841 gtggaaggcc tgagcaagca cattaagctg gaagaggaaa gaaggagcga ggacgcccaa
     6901 tctaaagccg ctctgaccga ttggctgaga gccaaggcca gctttgtgat cgagggcctg
     6961 aaagaggccg acaaggacga gttctgcaga tgcgagctga agctgcagaa gtggtacggc
     7021 gatctgagag gcaagccctt cgccattgag gccgagaaca gcatcctgga catcagcggc
     7081 ttcagcaagc agtacaactg cgccttcatt tggcagaaag acggcgtcaa gaaactgaac
     7141 ctgtacctga tcatcaatta cttcaaaggc ggcaagctgc ggttcaagaa gatcaaaccc
     7201 gaggccttcg aggctaacag attctacacc gtgatcaaca aaaagtccgg cgagatc
//
