LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             1198..2058
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        3032..3411
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        3412..3615
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   141..281
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    6774..6885
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        1093..1197
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      2229..2817
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      356..811
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3663..3689
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        join(7019..7257,1..2)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(286..337)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             6690..6712
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(812..861)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(963..998)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             6660..6682
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   2890..2917
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   2946..2973
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 acgaaacacc gactggcgct tttatctgat tactttgaga gccatcacca gcgactatgt
       61 cgtagtgggt aaagctccct cttcggaggg agcatcaaag gcgccgacgg gacgtaaaca
      121 tttttgccgt ttgcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
      181 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
      241 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
      301 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
      361 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      421 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      481 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      541 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
      601 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      661 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
      721 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      781 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
      841 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      901 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      961 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     1021 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1081 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1141 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1201 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1261 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1321 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1381 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1441 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1501 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1561 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1621 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1681 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1741 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1801 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1861 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggaagccgc
     1921 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1981 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2041 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2101 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2161 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2221 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2281 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2341 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2401 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2461 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2521 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2581 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2641 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2701 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2761 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2821 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc
     2881 tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc gggcgtcggg cgacctttgg
     2941 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     3001 gggttcctgc ggcctctaga ctcgaggcgt tgacattgat tattgactag ttattaatag
     3061 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3421 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3481 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     3541 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     3601 tatataagca gagctctctg gctaactacc ggttctagag cgctgccacc atgtccggat
     3661 cccctgctgc caagagggtc aagttggaca tgcaagagat caagagaatc aacaagatca
     3721 gaaggagact ggtcaaggac agcaacacaa agaaggccgg caagcgcggc cccatgaaaa
     3781 ccctgctcgt cagagtgatg acccctgacc tgagagagcg gctggaaaac ctgagaaaga
     3841 agcccgagaa catccctcag cctatcagca acaccagcag ggccaacctg aacaagctgc
     3901 tgaccgacta caccgagatg aagaaagcca tcctgcacgt gtactgggaa gagttccaga
     3961 aagaccccgt gggcctgatg agcagagttg ctcagcccgc tcctaagaac atcgaccaga
     4021 gaaagctgat ccccgtgaag gacggcaacg agagactgac ctctagcggc tttgcctgca
     4081 gccagtgttg ccagcctctg tacgtgtaca agctggaaca agtgaacgac aagggcaagc
     4141 cccacaccaa ctacttcggc agatgcaacg tgtccgagca cgagaggctg atcctgctgt
     4201 ctcctcacaa gcccgaggcc aacgatgagc tggtcacata cagcctgggc aagttcggac
     4261 agagagccct ggacttctac agcatccacg tgaccaggga gagcaatcac cctgtgaagc
     4321 ccctggaaca gatcggcggc aatagctgtg cctctggacc tgtgggaaaa gccctgagcg
     4381 acgcctgtat gggagccgtg gcatccttcc tgaccaagta ccaggacatc atcctggaac
     4441 accagaaagt gatcaagaag aacgagaaaa gactggccaa cctcaaggat atcgccagcg
     4501 ctaacggcct ggcctttcct aagatcaccc tgcctccaca gcctcacacc aaagagggca
     4561 tcgaggccta caacaacgtg gtggcccaga tcgtgatttg ggtcaacctg aatctgtggc
     4621 agaagctgaa gatcggcagg gacgaagcca agccactgca gagactgaag ggcttcccta
     4681 gcttccctct ggtggaaaga caggccaatg aagtggattg gtgggacatg gtctgcaacg
     4741 tgaagaagct gatcaacgag aagaaagagg atggcaaggt tttctggcag aacctggccg
     4801 gctacaagag acaagaagcc ctgctgcctt acctgagcag cgaagaggac cggaagaagg
     4861 gcaagaagtt cgccagatac cagttcggcg acctgctgct gcacctggaa aagaagcacg
     4921 gcgaggactg gggcaaagtg tacgatgagg cctgggagag aatcgacaag aaggtggaag
     4981 gcctgagcaa gcacattaag ctggaagagg aaagaaggag cgaggacgcc caatctaaag
     5041 ccgctctgac cgattggctg agagccaagg ccagctttgt gatcgagggc ctgaaagagg
     5101 ccgacaagga cgagttctgc agatgcgagc tgaagctgca gaagtggtac ggcgatctga
     5161 gaggcaagcc cttcgccatt gaggccgaga acagcatcct ggacatcagc ggcttcagca
     5221 agcagtacaa ctgcgccttc atttggcaga aagacggcgt caagaaactg aacctgtacc
     5281 tgatcatcaa ttacttcaaa ggcggcaagc tgcggttcaa gaagatcaaa cccgaggcct
     5341 tcgaggctaa cagattctac accgtgatca acaaaaagtc cggcgagatc gtgcccatgg
     5401 aagtgaactt caacttcgac gaccccaacc tgattatcct gcctctggcc ttcggcaaga
     5461 gacagggcag agagttcatc tggaacgatc tgctgagcct ggaaaccggc tctctgcgcc
     5521 tggccaatgg cagagtgatc gagaaaaccc tgtacaacag gagaaccaga caggacgagc
     5581 ctgctctgtt tgtggccctg accttcgaga gaagagaggt gctggacagc agcaacatca
     5641 agcccatgaa cctgatcggc atcgaccggg gcgagaatat ccctgctgtg atcgccctga
     5701 cagaccctga aggatgccca ctgagcagat tcaaggactc cctgggcaac cctacacaca
     5761 tcctgagaat cggcgagagc tacaaagaga agcagaggac aatccaggcc gccaaagagg
     5821 tggaacagag aagagccggc ggatactcta ggaagtacgc cagcaaggcc aagaatctgg
     5881 ccgacgacat ggtccgaaac accgccagag atctgctgta ctacgccgtg acacaggacg
     5941 ccatgctgat cttcgagaat ctgagcagag gcttcggccg gcagggcaag agaaccttta
     6001 tggccgagag gcagtacacc agaatggaag attggctcac agctaaactg gcctacgagg
     6061 gactgcccag caagacctac ctgtccaaaa cactggccca gtatacctcc cgcacctgca
     6121 gcaattgcgg cttcaccatc accagcgccg actacgacag agtgctggaa aagctcaaga
     6181 aaaccgccac cggctggatg accaccatca acggcaaaga gctgaaggtt gagggccaga
     6241 tcacctacta caacaggtac aagaggcaga acgtcgtgaa ggatctgagc gtggaactgg
     6301 acagactgag cgaagagagc gtgaacaacg acatcagcag ctggacaaag ggcagatcag
     6361 gcgaggctct gagcctgctg aagaagaggt ttagccacag acctgtgcaa gagaagttcg
     6421 tgtgcctgaa ctgcggcttc gagacacacg ccgatgaaca ggctgccctg aacattgcca
     6481 gaagctggct gttcctgaga agccaagagt acaagaagta ccagaccaac aagaccaccg
     6541 gcaacaccga caagagggcc tttgtggaaa cctggcagag cttctacaga aaaaagctga
     6601 aagaagtctg gaagcccgcc gtgaatgcat tgccaaagaa gaagcggaag gtcggcagtt
     6661 acccatacga tgtgcctgac tacgcttatc catacgacgt acccgattac gcctatcctt
     6721 acgacgtacc tgactacgcc taagaattcc tagagctcgc tgatcagcct cgactgtgcc
     6781 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6841 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6901 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6961 gaatagcagg catgctgggg aggtaccctt ttttggtacg gccgcctagt ctggccgcga
     7021 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     7081 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     7141 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     7201 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaagg
//
