LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             706..1566
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        2540..2919
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        2920..3123
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   6906..7046
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    6282..6393
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        601..705
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      1737..2325
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      join(7121..7257,1..319)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3171..3197
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        6527..6767
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(7051..7102)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             6198..6220
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(320..369)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(471..506)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             6168..6190
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   2398..2425
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   2454..2481
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
       61 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      121 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      181 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      241 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      301 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
      361 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
      421 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
      481 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
      541 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      601 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      661 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      721 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      781 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      841 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      901 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      961 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1021 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1081 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1141 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1201 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1261 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1321 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1381 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca
     1441 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1501 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1561 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1621 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1861 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1921 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1981 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2041 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2101 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2161 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2221 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2281 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2341 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg
     2401 cgcgctcgct cgctcactga ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc
     2461 agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg
     2521 cctctagact cgaggcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2581 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctctctggc taactaccgg ttctagagcg ctgccaccat gtccggatcc cctgctgcca
     3181 agagggtcaa gttggacatg caagagatca agagaatcaa caagatcaga aggagactgg
     3241 tcaaggacag caacacaaag aaggccggca agcgcggccc catgaaaacc ctgctcgtca
     3301 gagtgatgac ccctgacctg agagagcggc tggaaaacct gagaaagaag cccgagaaca
     3361 tccctcagcc tatcagcaac accagcaggg ccaacctgaa caagctgctg accgactaca
     3421 ccgagatgaa gaaagccatc ctgcacgtgt actgggaaga gttccagaaa gaccccgtgg
     3481 gcctgatgag cagagttgct cagcccgctc ctaagaacat cgaccagaga aagctgatcc
     3541 ccgtgaagga cggcaacgag agactgacct ctagcggctt tgcctgcagc cagtgttgcc
     3601 agcctctgta cgtgtacaag ctggaacaag tgaacgacaa gggcaagccc cacaccaact
     3661 acttcggcag atgcaacgtg tccgagcacg agaggctgat cctgctgtct cctcacaagc
     3721 ccgaggccaa cgatgagctg gtcacataca gcctgggcaa gttcggacag agagccctgg
     3781 acttctacag catccacgtg accagggaga gcaatcaccc tgtgaagccc ctggaacaga
     3841 tcggcggcaa tagctgtgcc tctggacctg tgggaaaagc cctgagcgac gcctgtatgg
     3901 gagccgtggc atccttcctg accaagtacc aggacatcat cctggaacac cagaaagtga
     3961 tcaagaagaa cgagaaaaga ctggccaacc tcaaggatat cgccagcgct aacggcctgg
     4021 cctttcctaa gatcaccctg cctccacagc ctcacaccaa agagggcatc gaggcctaca
     4081 acaacgtggt ggcccagatc gtgatttggg tcaacctgaa tctgtggcag aagctgaaga
     4141 tcggcaggga cgaagccaag ccactgcaga gactgaaggg cttccctagc ttccctctgg
     4201 tggaaagaca ggccaatgaa gtggattggt gggacatggt ctgcaacgtg aagaagctga
     4261 tcaacgagaa gaaagaggat ggcaaggttt tctggcagaa cctggccggc tacaagagac
     4321 aagaagccct gctgccttac ctgagcagcg aagaggaccg gaagaagggc aagaagttcg
     4381 ccagatacca gttcggcgac ctgctgctgc acctggaaaa gaagcacggc gaggactggg
     4441 gcaaagtgta cgatgaggcc tgggagagaa tcgacaagaa ggtggaaggc ctgagcaagc
     4501 acattaagct ggaagaggaa agaaggagcg aggacgccca atctaaagcc gctctgaccg
     4561 attggctgag agccaaggcc agctttgtga tcgagggcct gaaagaggcc gacaaggacg
     4621 agttctgcag atgcgagctg aagctgcaga agtggtacgg cgatctgaga ggcaagccct
     4681 tcgccattga ggccgagaac agcatcctgg acatcagcgg cttcagcaag cagtacaact
     4741 gcgccttcat ttggcagaaa gacggcgtca agaaactgaa cctgtacctg atcatcaatt
     4801 acttcaaagg cggcaagctg cggttcaaga agatcaaacc cgaggccttc gaggctaaca
     4861 gattctacac cgtgatcaac aaaaagtccg gcgagatcgt gcccatggaa gtgaacttca
     4921 acttcgacga ccccaacctg attatcctgc ctctggcctt cggcaagaga cagggcagag
     4981 agttcatctg gaacgatctg ctgagcctgg aaaccggctc tctgcgcctg gccaatggca
     5041 gagtgatcga gaaaaccctg tacaacagga gaaccagaca ggacgagcct gctctgtttg
     5101 tggccctgac cttcgagaga agagaggtgc tggacagcag caacatcaag cccatgaacc
     5161 tgatcggcat cgaccggggc gagaatatcc ctgctgtgat cgccctgaca gaccctgaag
     5221 gatgcccact gagcagattc aaggactccc tgggcaaccc tacacacatc ctgagaatcg
     5281 gcgagagcta caaagagaag cagaggacaa tccaggccgc caaagaggtg gaacagagaa
     5341 gagccggcgg atactctagg aagtacgcca gcaaggccaa gaatctggcc gacgacatgg
     5401 tccgaaacac cgccagagat ctgctgtact acgccgtgac acaggacgcc atgctgatct
     5461 tcgagaatct gagcagaggc ttcggccggc agggcaagag aacctttatg gccgagaggc
     5521 agtacaccag aatggaagat tggctcacag ctaaactggc ctacgaggga ctgcccagca
     5581 agacctacct gtccaaaaca ctggcccagt atacctcccg cacctgcagc aattgcggct
     5641 tcaccatcac cagcgccgac tacgacagag tgctggaaaa gctcaagaaa accgccaccg
     5701 gctggatgac caccatcaac ggcaaagagc tgaaggttga gggccagatc acctactaca
     5761 acaggtacaa gaggcagaac gtcgtgaagg atctgagcgt ggaactggac agactgagcg
     5821 aagagagcgt gaacaacgac atcagcagct ggacaaaggg cagatcaggc gaggctctga
     5881 gcctgctgaa gaagaggttt agccacagac ctgtgcaaga gaagttcgtg tgcctgaact
     5941 gcggcttcga gacacacgcc gatgaacagg ctgccctgaa cattgccaga agctggctgt
     6001 tcctgagaag ccaagagtac aagaagtacc agaccaacaa gaccaccggc aacaccgaca
     6061 agagggcctt tgtggaaacc tggcagagct tctacagaaa aaagctgaaa gaagtctgga
     6121 agcccgccgt gaatgcattg ccaaagaaga agcggaaggt cggcagttac ccatacgatg
     6181 tgcctgacta cgcttatcca tacgacgtac ccgattacgc ctatccttac gacgtacctg
     6241 actacgccta agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     6301 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6361 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6421 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     6481 tgctggggag gtaccctttt ttggtacggc cgcctagtct ggccgcgagg gcctatttcc
     6541 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
     6601 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     6661 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     6721 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgactg
     6781 gcgcttttat ctgattactt tgagagccat caccagcgac tatgtcgtag tgggtaaagc
     6841 tccctcttcg gagggagcat caaaggcgcc gacgggacgt aaacattttt gccgtttgcg
     6901 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     6961 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     7021 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     7081 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     7141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
     7201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccc
//
