LOCUS       plasmid                 7140 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     enhancer        906..1285
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        1286..1489
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   5272..5412
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    4648..4759
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6224..6328
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      103..691
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             6329..6957
                     /note="pLannotate"
                     /label="AmpR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="73.1"
                     /fragment="True"
                     /other="CDS"
     rep_origin      5487..5942
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1537..1563
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4893..5133
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(5417..5468)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             4564..4586
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5943..5992)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(6094..6129)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             4534..4556
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   764..791
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   820..847
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
       61 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      481 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      721 cctggccttt tgctggcctt ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct
      781 cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag
      841 cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggcctc tagactcgag
      901 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      961 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1021 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1081 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1141 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1201 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1261 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1321 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1381 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1441 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     1501 taccggttct agagcgctgc caccatgtcc ggatcccctg ctgccaagag ggtcaagttg
     1561 gacatgcaag agatcaagag aatcaacaag atcagaagga gactggtcaa ggacagcaac
     1621 acaaagaagg ccggcaagcg cggccccatg aaaaccctgc tcgtcagagt gatgacccct
     1681 gacctgagag agcggctgga aaacctgaga aagaagcccg agaacatccc tcagcctatc
     1741 agcaacacca gcagggccaa cctgaacaag ctgctgaccg actacaccga gatgaagaaa
     1801 gccatcctgc acgtgtactg ggaagagttc cagaaagacc ccgtgggcct gatgagcaga
     1861 gttgctcagc ccgctcctaa gaacatcgac cagagaaagc tgatccccgt gaaggacggc
     1921 aacgagagac tgacctctag cggctttgcc tgcagccagt gttgccagcc tctgtacgtg
     1981 tacaagctgg aacaagtgaa cgacaagggc aagccccaca ccaactactt cggcagatgc
     2041 aacgtgtccg agcacgagag gctgatcctg ctgtctcctc acaagcccga ggccaacgat
     2101 gagctggtca catacagcct gggcaagttc ggacagagag ccctggactt ctacagcatc
     2161 cacgtgacca gggagagcaa tcaccctgtg aagcccctgg aacagatcgg cggcaatagc
     2221 tgtgcctctg gacctgtggg aaaagccctg agcgacgcct gtatgggagc cgtggcatcc
     2281 ttcctgacca agtaccagga catcatcctg gaacaccaga aagtgatcaa gaagaacgag
     2341 aaaagactgg ccaacctcaa ggatatcgcc agcgctaacg gcctggcctt tcctaagatc
     2401 accctgcctc cacagcctca caccaaagag ggcatcgagg cctacaacaa cgtggtggcc
     2461 cagatcgtga tttgggtcaa cctgaatctg tggcagaagc tgaagatcgg cagggacgaa
     2521 gccaagccac tgcagagact gaagggcttc cctagcttcc ctctggtgga aagacaggcc
     2581 aatgaagtgg attggtggga catggtctgc aacgtgaaga agctgatcaa cgagaagaaa
     2641 gaggatggca aggttttctg gcagaacctg gccggctaca agagacaaga agccctgctg
     2701 ccttacctga gcagcgaaga ggaccggaag aagggcaaga agttcgccag ataccagttc
     2761 ggcgacctgc tgctgcacct ggaaaagaag cacggcgagg actggggcaa agtgtacgat
     2821 gaggcctggg agagaatcga caagaaggtg gaaggcctga gcaagcacat taagctggaa
     2881 gaggaaagaa ggagcgagga cgcccaatct aaagccgctc tgaccgattg gctgagagcc
     2941 aaggccagct ttgtgatcga gggcctgaaa gaggccgaca aggacgagtt ctgcagatgc
     3001 gagctgaagc tgcagaagtg gtacggcgat ctgagaggca agcccttcgc cattgaggcc
     3061 gagaacagca tcctggacat cagcggcttc agcaagcagt acaactgcgc cttcatttgg
     3121 cagaaagacg gcgtcaagaa actgaacctg tacctgatca tcaattactt caaaggcggc
     3181 aagctgcggt tcaagaagat caaacccgag gccttcgagg ctaacagatt ctacaccgtg
     3241 atcaacaaaa agtccggcga gatcgtgccc atggaagtga acttcaactt cgacgacccc
     3301 aacctgatta tcctgcctct ggccttcggc aagagacagg gcagagagtt catctggaac
     3361 gatctgctga gcctggaaac cggctctctg cgcctggcca atggcagagt gatcgagaaa
     3421 accctgtaca acaggagaac cagacaggac gagcctgctc tgtttgtggc cctgaccttc
     3481 gagagaagag aggtgctgga cagcagcaac atcaagccca tgaacctgat cggcatcgac
     3541 cggggcgaga atatccctgc tgtgatcgcc ctgacagacc ctgaaggatg cccactgagc
     3601 agattcaagg actccctggg caaccctaca cacatcctga gaatcggcga gagctacaaa
     3661 gagaagcaga ggacaatcca ggccgccaaa gaggtggaac agagaagagc cggcggatac
     3721 tctaggaagt acgccagcaa ggccaagaat ctggccgacg acatggtccg aaacaccgcc
     3781 agagatctgc tgtactacgc cgtgacacag gacgccatgc tgatcttcga gaatctgagc
     3841 agaggcttcg gccggcaggg caagagaacc tttatggccg agaggcagta caccagaatg
     3901 gaagattggc tcacagctaa actggcctac gagggactgc ccagcaagac ctacctgtcc
     3961 aaaacactgg cccagtatac ctcccgcacc tgcagcaatt gcggcttcac catcaccagc
     4021 gccgactacg acagagtgct ggaaaagctc aagaaaaccg ccaccggctg gatgaccacc
     4081 atcaacggca aagagctgaa ggttgagggc cagatcacct actacaacag gtacaagagg
     4141 cagaacgtcg tgaaggatct gagcgtggaa ctggacagac tgagcgaaga gagcgtgaac
     4201 aacgacatca gcagctggac aaagggcaga tcaggcgagg ctctgagcct gctgaagaag
     4261 aggtttagcc acagacctgt gcaagagaag ttcgtgtgcc tgaactgcgg cttcgagaca
     4321 cacgccgatg aacaggctgc cctgaacatt gccagaagct ggctgttcct gagaagccaa
     4381 gagtacaaga agtaccagac caacaagacc accggcaaca ccgacaagag ggcctttgtg
     4441 gaaacctggc agagcttcta cagaaaaaag ctgaaagaag tctggaagcc cgccgtgaat
     4501 gcattgccaa agaagaagcg gaaggtcggc agttacccat acgatgtgcc tgactacgct
     4561 tatccatacg acgtacccga ttacgcctat ccttacgacg tacctgacta cgcctaagaa
     4621 ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     4681 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     4741 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     4801 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggaggtac
     4861 ccttttttgg tacggccgcc tagtctggcc gcgagggcct atttcccatg attccttcat
     4921 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     4981 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     5041 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     5101 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgactggcgc ttttatctga
     5161 ttactttgag agccatcacc agcgactatg tcgtagtggg taaagctccc tcttcggagg
     5221 gagcatcaaa gcttcacctc tgcacgtcgc atttttgccg tttgcggccg caggaacccc
     5281 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     5341 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     5401 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5461 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     5521 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     5581 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5641 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5701 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5761 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     5821 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     5881 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5941 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6001 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6061 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6121 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6181 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6901 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaaac
     6961 cactggtgca gctttgaaca tctaggtttt cgcatttatc gtgaaacgct ttcgcgtttt
     7021 taaccactgg tgcagctttg aacatctagg ttttcgcatt tatcgtgaaa cgctttcgcg
     7081 ttttttcgtg cgccgcttca tttagattga tttaaaactt catttttaat ttaaaaggat
//
