LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             3283..4143
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        5117..5496
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        5497..5700
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   2226..2366
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    1602..1713
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        3178..3282
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      4314..4902
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      2441..2896
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             5748..5774
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        1847..2087
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(2371..2422)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1518..1540
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(2897..2946)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3048..3083)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1488..1510
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   4975..5002
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   5031..5058
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 tcgccattga ggccgagaac agcatcctgg acatcagcgg cttcagcaag cagtacaact
       61 gcgccttcat ttggcagaaa gacggcgtca agaaactgaa cctgtacctg atcatcaatt
      121 acttcaaagg cggcaagctg cggttcaaga agatcaaacc cgaggccttc gaggctaaca
      181 gattctacac cgtgatcaac aaaaagtccg gcgagatcgt gcccatggaa gtgaacttca
      241 acttcgacga ccccaacctg attatcctgc ctctggcctt cggcaagaga cagggcagag
      301 agttcatctg gaacgatctg ctgagcctgg aaaccggctc tctgcgcctg gccaatggca
      361 gagtgatcga gaaaaccctg tacaacagga gaaccagaca ggacgagcct gctctgtttg
      421 tggccctgac cttcgagaga agagaggtgc tggacagcag caacatcaag cccatgaacc
      481 tgatcggcat cgaccggggc gagaatatcc ctgctgtgat cgccctgaca gaccctgaag
      541 gatgcccact gagcagattc aaggactccc tgggcaaccc tacacacatc ctgagaatcg
      601 gcgagagcta caaagagaag cagaggacaa tccaggccgc caaagaggtg gaacagagaa
      661 gagccggcgg atactctagg aagtacgcca gcaaggccaa gaatctggcc gacgacatgg
      721 tccgaaacac cgccagagat ctgctgtact acgccgtgac acaggacgcc atgctgatct
      781 tcgagaatct gagcagaggc ttcggccggc agggcaagag aacctttatg gccgagaggc
      841 agtacaccag aatggaagat tggctcacag ctaaactggc ctacgaggga ctgcccagca
      901 agacctacct gtccaaaaca ctggcccagt atacctcccg cacctgcagc aattgcggct
      961 tcaccatcac cagcgccgac tacgacagag tgctggaaaa gctcaagaaa accgccaccg
     1021 gctggatgac caccatcaac ggcaaagagc tgaaggttga gggccagatc acctactaca
     1081 acaggtacaa gaggcagaac gtcgtgaagg atctgagcgt ggaactggac agactgagcg
     1141 aagagagcgt gaacaacgac atcagcagct ggacaaaggg cagatcaggc gaggctctga
     1201 gcctgctgaa gaagaggttt agccacagac ctgtgcaaga gaagttcgtg tgcctgaact
     1261 gcggcttcga gacacacgcc gatgaacagg ctgccctgaa cattgccaga agctggctgt
     1321 tcctgagaag ccaagagtac aagaagtacc agaccaacaa gaccaccggc aacaccgaca
     1381 agagggcctt tgtggaaacc tggcagagct tctacagaaa aaagctgaaa gaagtctgga
     1441 agcccgccgt gaatgcattg ccaaagaaga agcggaaggt cggcagttac ccatacgatg
     1501 tgcctgacta cgcttatcca tacgacgtac ccgattacgc ctatccttac gacgtacctg
     1561 actacgccta agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     1621 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1681 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1741 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     1801 tgctggggag gtaccctttt ttggtacggc cgcctagtct ggccgcgagg gcctatttcc
     1861 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
     1921 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     1981 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     2041 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgactg
     2101 gcgcttttat ctgattactt tgagagccat caccagcgac tatgtcgtag tgggtaaagc
     2161 tccctcttcg gagggagcat caaagcttca cctctgcacg tcgcattttt gccgtttgcg
     2221 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     2281 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     2341 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     2401 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     2461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
     2521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2581 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2641 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2701 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2761 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     2821 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2881 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     2941 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3001 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3061 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3121 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3961 tggtttattg ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca
     4021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4441 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc
     4981 gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
     5041 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggcct
     5101 ctagactcga ggcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     5161 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     5221 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     5281 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     5341 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     5401 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     5461 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     5521 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     5581 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     5641 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     5701 ctctggctaa ctaccggttc tagagcgctg ccaccatgtc cggatcccct gctgccaaga
     5761 gggtcaagtt ggacatgcaa gagatcaaga gaatcaacaa gatcagaagg agactggtca
     5821 aggacagcaa cacaaagaag gccggcaagc gcggccccat gaaaaccctg ctcgtcagag
     5881 tgatgacccc tgacctgaga gagcggctgg aaaacctgag aaagaagccc gagaacatcc
     5941 ctcagcctat cagcaacacc agcagggcca acctgaacaa gctgctgacc gactacaccg
     6001 agatgaagaa agccatcctg cacgtgtact gggaagagtt ccagaaagac cccgtgggcc
     6061 tgatgagcag agttgctcag cccgctccta agaacatcga ccagagaaag ctgatccccg
     6121 tgaaggacgg caacgagaga ctgacctcta gcggctttgc ctgcagccag tgttgccagc
     6181 ctctgtacgt gtacaagctg gaacaagtga acgacaaggg caagccccac accaactact
     6241 tcggcagatg caacgtgtcc gagcacgaga ggctgatcct gctgtctcct cacaagcccg
     6301 aggccaacga tgagctggtc acatacagcc tgggcaagtt cggacagaga gccctggact
     6361 tctacagcat ccacgtgacc agggagagca atcaccctgt gaagcccctg gaacagatcg
     6421 gcggcaatag ctgtgcctct ggacctgtgg gaaaagccct gagcgacgcc tgtatgggag
     6481 ccgtggcatc cttcctgacc aagtaccagg acatcatcct ggaacaccag aaagtgatca
     6541 agaagaacga gaaaagactg gccaacctca aggatatcgc cagcgctaac ggcctggcct
     6601 ttcctaagat caccctgcct ccacagcctc acaccaaaga gggcatcgag gcctacaaca
     6661 acgtggtggc ccagatcgtg atttgggtca acctgaatct gtggcagaag ctgaagatcg
     6721 gcagggacga agccaagcca ctgcagagac tgaagggctt ccctagcttc cctctggtgg
     6781 aaagacaggc caatgaagtg gattggtggg acatggtctg caacgtgaag aagctgatca
     6841 acgagaagaa agaggatggc aaggttttct ggcagaacct ggccggctac aagagacaag
     6901 aagccctgct gccttacctg agcagcgaag aggaccggaa gaagggcaag aagttcgcca
     6961 gataccagtt cggcgacctg ctgctgcacc tggaaaagaa gcacggcgag gactggggca
     7021 aagtgtacga tgaggcctgg gagagaatcg acaagaaggt ggaaggcctg agcaagcaca
     7081 ttaagctgga agaggaaaga aggagcgagg acgcccaatc taaagccgct ctgaccgatt
     7141 ggctgagagc caaggccagc tttgtgatcg agggcctgaa agaggccgac aaggacgagt
     7201 tctgcagatg cgagctgaag ctgcagaagt ggtacggcga tctgagaggc aagccct
//
