LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             5455..6315
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        32..411
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        412..615
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   4398..4538
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    3774..3885
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5350..5454
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      6486..7074
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      4613..5068
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             663..689
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4019..4259
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(4543..4594)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3690..3712
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5069..5118)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(5220..5255)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             3660..3682
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   7147..7174
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   7203..7230
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 gggttcctgc ggcctctaga ctcgaggcgt tgacattgat tattgactag ttattaatag
       61 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      421 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      481 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      541 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      601 tatataagca gagctctctg gctaactacc ggttctagag cgctgccacc atgtccggat
      661 cccctgctgc caagagggtc aagttggaca tgcaagagat caagagaatc aacaagatca
      721 gaaggagact ggtcaaggac agcaacacaa agaaggccgg caagcgcggc cccatgaaaa
      781 ccctgctcgt cagagtgatg acccctgacc tgagagagcg gctggaaaac ctgagaaaga
      841 agcccgagaa catccctcag cctatcagca acaccagcag ggccaacctg aacaagctgc
      901 tgaccgacta caccgagatg aagaaagcca tcctgcacgt gtactgggaa gagttccaga
      961 aagaccccgt gggcctgatg agcagagttg ctcagcccgc tcctaagaac atcgaccaga
     1021 gaaagctgat ccccgtgaag gacggcaacg agagactgac ctctagcggc tttgcctgca
     1081 gccagtgttg ccagcctctg tacgtgtaca agctggaaca agtgaacgac aagggcaagc
     1141 cccacaccaa ctacttcggc agatgcaacg tgtccgagca cgagaggctg atcctgctgt
     1201 ctcctcacaa gcccgaggcc aacgatgagc tggtcacata cagcctgggc aagttcggac
     1261 agagagccct ggacttctac agcatccacg tgaccaggga gagcaatcac cctgtgaagc
     1321 ccctggaaca gatcggcggc aatagctgtg cctctggacc tgtgggaaaa gccctgagcg
     1381 acgcctgtat gggagccgtg gcatccttcc tgaccaagta ccaggacatc atcctggaac
     1441 accagaaagt gatcaagaag aacgagaaaa gactggccaa cctcaaggat atcgccagcg
     1501 ctaacggcct ggcctttcct aagatcaccc tgcctccaca gcctcacacc aaagagggca
     1561 tcgaggccta caacaacgtg gtggcccaga tcgtgatttg ggtcaacctg aatctgtggc
     1621 agaagctgaa gatcggcagg gacgaagcca agccactgca gagactgaag ggcttcccta
     1681 gcttccctct ggtggaaaga caggccaatg aagtggattg gtgggacatg gtctgcaacg
     1741 tgaagaagct gatcaacgag aagaaagagg atggcaaggt tttctggcag aacctggccg
     1801 gctacaagag acaagaagcc ctgctgcctt acctgagcag cgaagaggac cggaagaagg
     1861 gcaagaagtt cgccagatac cagttcggcg acctgctgct gcacctggaa aagaagcacg
     1921 gcgaggactg gggcaaagtg tacgatgagg cctgggagag aatcgacaag aaggtggaag
     1981 gcctgagcaa gcacattaag ctggaagagg aaagaaggag cgaggacgcc caatctaaag
     2041 ccgctctgac cgattggctg agagccaagg ccagctttgt gatcgagggc ctgaaagagg
     2101 ccgacaagga cgagttctgc agatgcgagc tgaagctgca gaagtggtac ggcgatctga
     2161 gaggcaagcc cttcgccatt gaggccgaga acagcatcct ggacatcagc ggcttcagca
     2221 agcagtacaa ctgcgccttc atttggcaga aagacggcgt caagaaactg aacctgtacc
     2281 tgatcatcaa ttacttcaaa ggcggcaagc tgcggttcaa gaagatcaaa cccgaggcct
     2341 tcgaggctaa cagattctac accgtgatca acaaaaagtc cggcgagatc gtgcccatgg
     2401 aagtgaactt caacttcgac gaccccaacc tgattatcct gcctctggcc ttcggcaaga
     2461 gacagggcag agagttcatc tggaacgatc tgctgagcct ggaaaccggc tctctgcgcc
     2521 tggccaatgg cagagtgatc gagaaaaccc tgtacaacag gagaaccaga caggacgagc
     2581 ctgctctgtt tgtggccctg accttcgaga gaagagaggt gctggacagc agcaacatca
     2641 agcccatgaa cctgatcggc atcgaccggg gcgagaatat ccctgctgtg atcgccctga
     2701 cagaccctga aggatgccca ctgagcagat tcaaggactc cctgggcaac cctacacaca
     2761 tcctgagaat cggcgagagc tacaaagaga agcagaggac aatccaggcc gccaaagagg
     2821 tggaacagag aagagccggc ggatactcta ggaagtacgc cagcaaggcc aagaatctgg
     2881 ccgacgacat ggtccgaaac accgccagag atctgctgta ctacgccgtg acacaggacg
     2941 ccatgctgat cttcgagaat ctgagcagag gcttcggccg gcagggcaag agaaccttta
     3001 tggccgagag gcagtacacc agaatggaag attggctcac agctaaactg gcctacgagg
     3061 gactgcccag caagacctac ctgtccaaaa cactggccca gtatacctcc cgcacctgca
     3121 gcaattgcgg cttcaccatc accagcgccg actacgacag agtgctggaa aagctcaaga
     3181 aaaccgccac cggctggatg accaccatca acggcaaaga gctgaaggtt gagggccaga
     3241 tcacctacta caacaggtac aagaggcaga acgtcgtgaa ggatctgagc gtggaactgg
     3301 acagactgag cgaagagagc gtgaacaacg acatcagcag ctggacaaag ggcagatcag
     3361 gcgaggctct gagcctgctg aagaagaggt ttagccacag acctgtgcaa gagaagttcg
     3421 tgtgcctgaa ctgcggcttc gagacacacg ccgatgaaca ggctgccctg aacattgcca
     3481 gaagctggct gttcctgaga agccaagagt acaagaagta ccagaccaac aagaccaccg
     3541 gcaacaccga caagagggcc tttgtggaaa cctggcagag cttctacaga aaaaagctga
     3601 aagaagtctg gaagcccgcc gtgaatgcat tgccaaagaa gaagcggaag gtcggcagtt
     3661 acccatacga tgtgcctgac tacgcttatc catacgacgt acccgattac gcctatcctt
     3721 acgacgtacc tgactacgcc taagaattcc tagagctcgc tgatcagcct cgactgtgcc
     3781 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     3841 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     3901 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     3961 gaatagcagg catgctgggg aggtaccctt ttttggtacg gccgcctagt ctggccgcga
     4021 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     4081 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     4141 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     4201 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     4261 aaacaccgac tggcgctttt atctgattac tttgagagcc atcaccagcg actatgtcgt
     4321 agtgggtaaa gctccctctt cggagggagc atcaaagcct ctgcacgtcg catggagttt
     4381 ttgccgtttg cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4441 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4501 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     4561 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4681 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4801 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4921 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     4981 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5041 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     5101 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5161 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5221 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5281 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt
     6181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc
     7141 aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg
     7201 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactag
//
