LOCUS       plasmid                 7257 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             503..1363
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        2337..2716
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        2717..2920
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   6703..6843
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    6079..6190
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        398..502
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      1534..2122
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      join(6918..7257,1..116)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2968..2994
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        6324..6564
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(6848..6899)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             5995..6017
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(117..166)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(268..303)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             5965..5987
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   2195..2222
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   2251..2278
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
       61 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
      121 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      181 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      241 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      301 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      361 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1681 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2101 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2161 ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc
     2221 cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
     2281 agggagtggc caactccatc actaggggtt cctgcggcct ctagactcga ggcgttgaca
     2341 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2401 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2461 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2521 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2581 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2641 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2701 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2761 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2821 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2881 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctaccggttc
     2941 tagagcgctg ccaccatgtc cggatcccct gctgccaaga gggtcaagtt ggacatgcaa
     3001 gagatcaaga gaatcaacaa gatcagaagg agactggtca aggacagcaa cacaaagaag
     3061 gccggcaagc gcggccccat gaaaaccctg ctcgtcagag tgatgacccc tgacctgaga
     3121 gagcggctgg aaaacctgag aaagaagccc gagaacatcc ctcagcctat cagcaacacc
     3181 agcagggcca acctgaacaa gctgctgacc gactacaccg agatgaagaa agccatcctg
     3241 cacgtgtact gggaagagtt ccagaaagac cccgtgggcc tgatgagcag agttgctcag
     3301 cccgctccta agaacatcga ccagagaaag ctgatccccg tgaaggacgg caacgagaga
     3361 ctgacctcta gcggctttgc ctgcagccag tgttgccagc ctctgtacgt gtacaagctg
     3421 gaacaagtga acgacaaggg caagccccac accaactact tcggcagatg caacgtgtcc
     3481 gagcacgaga ggctgatcct gctgtctcct cacaagcccg aggccaacga tgagctggtc
     3541 acatacagcc tgggcaagtt cggacagaga gccctggact tctacagcat ccacgtgacc
     3601 agggagagca atcaccctgt gaagcccctg gaacagatcg gcggcaatag ctgtgcctct
     3661 ggacctgtgg gaaaagccct gagcgacgcc tgtatgggag ccgtggcatc cttcctgacc
     3721 aagtaccagg acatcatcct ggaacaccag aaagtgatca agaagaacga gaaaagactg
     3781 gccaacctca aggatatcgc cagcgctaac ggcctggcct ttcctaagat caccctgcct
     3841 ccacagcctc acaccaaaga gggcatcgag gcctacaaca acgtggtggc ccagatcgtg
     3901 atttgggtca acctgaatct gtggcagaag ctgaagatcg gcagggacga agccaagcca
     3961 ctgcagagac tgaagggctt ccctagcttc cctctggtgg aaagacaggc caatgaagtg
     4021 gattggtggg acatggtctg caacgtgaag aagctgatca acgagaagaa agaggatggc
     4081 aaggttttct ggcagaacct ggccggctac aagagacaag aagccctgct gccttacctg
     4141 agcagcgaag aggaccggaa gaagggcaag aagttcgcca gataccagtt cggcgacctg
     4201 ctgctgcacc tggaaaagaa gcacggcgag gactggggca aagtgtacga tgaggcctgg
     4261 gagagaatcg acaagaaggt ggaaggcctg agcaagcaca ttaagctgga agaggaaaga
     4321 aggagcgagg acgcccaatc taaagccgct ctgaccgatt ggctgagagc caaggccagc
     4381 tttgtgatcg agggcctgaa agaggccgac aaggacgagt tctgcagatg cgagctgaag
     4441 ctgcagaagt ggtacggcga tctgagaggc aagcccttcg ccattgaggc cgagaacagc
     4501 atcctggaca tcagcggctt cagcaagcag tacaactgcg ccttcatttg gcagaaagac
     4561 ggcgtcaaga aactgaacct gtacctgatc atcaattact tcaaaggcgg caagctgcgg
     4621 ttcaagaaga tcaaacccga ggccttcgag gctaacagat tctacaccgt gatcaacaaa
     4681 aagtccggcg agatcgtgcc catggaagtg aacttcaact tcgacgaccc caacctgatt
     4741 atcctgcctc tggccttcgg caagagacag ggcagagagt tcatctggaa cgatctgctg
     4801 agcctggaaa ccggctctct gcgcctggcc aatggcagag tgatcgagaa aaccctgtac
     4861 aacaggagaa ccagacagga cgagcctgct ctgtttgtgg ccctgacctt cgagagaaga
     4921 gaggtgctgg acagcagcaa catcaagccc atgaacctga tcggcatcga ccggggcgag
     4981 aatatccctg ctgtgatcgc cctgacagac cctgaaggat gcccactgag cagattcaag
     5041 gactccctgg gcaaccctac acacatcctg agaatcggcg agagctacaa agagaagcag
     5101 aggacaatcc aggccgccaa agaggtggaa cagagaagag ccggcggata ctctaggaag
     5161 tacgccagca aggccaagaa tctggccgac gacatggtcc gaaacaccgc cagagatctg
     5221 ctgtactacg ccgtgacaca ggacgccatg ctgatcttcg agaatctgag cagaggcttc
     5281 ggccggcagg gcaagagaac ctttatggcc gagaggcagt acaccagaat ggaagattgg
     5341 ctcacagcta aactggccta cgagggactg cccagcaaga cctacctgtc caaaacactg
     5401 gcccagtata cctcccgcac ctgcagcaat tgcggcttca ccatcaccag cgccgactac
     5461 gacagagtgc tggaaaagct caagaaaacc gccaccggct ggatgaccac catcaacggc
     5521 aaagagctga aggttgaggg ccagatcacc tactacaaca ggtacaagag gcagaacgtc
     5581 gtgaaggatc tgagcgtgga actggacaga ctgagcgaag agagcgtgaa caacgacatc
     5641 agcagctgga caaagggcag atcaggcgag gctctgagcc tgctgaagaa gaggtttagc
     5701 cacagacctg tgcaagagaa gttcgtgtgc ctgaactgcg gcttcgagac acacgccgat
     5761 gaacaggctg ccctgaacat tgccagaagc tggctgttcc tgagaagcca agagtacaag
     5821 aagtaccaga ccaacaagac caccggcaac accgacaaga gggcctttgt ggaaacctgg
     5881 cagagcttct acagaaaaaa gctgaaagaa gtctggaagc ccgccgtgaa tgcattgcca
     5941 aagaagaagc ggaaggtcgg cagttaccca tacgatgtgc ctgactacgc ttatccatac
     6001 gacgtacccg attacgccta tccttacgac gtacctgact acgcctaaga attcctagag
     6061 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6121 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6181 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6241 acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggaggta cccttttttg
     6301 gtacggccgc ctagtctggc cgcgagggcc tatttcccat gattccttca tatttgcata
     6361 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     6421 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     6481 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     6541 ctttatatat cttgtggaaa ggacgaaaca ccgactggcg cttttatctg attactttga
     6601 gagccatcac cagcgactat gtcgtagtgg gtaaagctcc ctcttcggag ggagcatcaa
     6661 agcctctgca cgtcgcatgg agtttttgcc gtttgcggcc gcaggaaccc ctagtgatgg
     6721 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     6781 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     6841 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     6901 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6961 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     7021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7081 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     7141 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7201 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctc
//
