LOCUS       plasmid                 9230 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     gene            1253..3260
                     /note="pLannotate"
                     /label="ptrA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="gene"
     promoter        3769..3873
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3874..4734
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             join(8985..9230,1..468)
                     /note="pLannotate"
                     /label="mgfp5"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     terminator      509..1237
                     /note="pLannotate"
                     /label="trpC terminator"
                     /database="snapgene"
                     /identity="97.4"
                     /match_length="95.7"
                     /fragment="False"
                     /other="terminator"
     rep_origin      4905..5493
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        5817..5847
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    5781..5802
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    5855..5871
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             3278..3432
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3438..3537)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             8664..8807
                     /note="pLannotate"
                     /label="YNG1 (fragment)"
                     /database="swissprot"
                     /identity="56.3"
                     /match_length="21.9"
                     /fragment="True"
                     /other="CDS"
     CDS             5676..5768
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3639..3674)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttcaagagcg ccatgcctga gggatacgtg caggagagga ccatcttctt caaagacgac
       61 gggaactaca agacacgtgc tgaagtcaag tttgagggag acaccctcgt caacaggatc
      121 gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa gttggaatac
      181 aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg catcaaagcc
      241 aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga tcattatcaa
      301 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      361 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      421 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataaac cggtcgcctc
      481 aaacaatgct ctgcggccgc aagcttgaga tccacttaac gttactgaaa tcatcaaaca
      541 gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag ttgagacaaa
      601 tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga gtgccttcta
      661 gtgatttaat agctccatgt caacaagaat aaaacgcgtt tcgggtttac ctcttccaga
      721 tacagctcat ctgcaatgca ttaatgcatt ggacctcgca accctagtac gcccttcagg
      781 ctccggcgaa gcagaagaat agcttagcag agtctatttt cattttcggg agacgagatc
      841 aagcagatca acggtcgtca agagacctac gagactgagg aatccgctct tggctccacg
      901 cgactatata tttgtctcta attgtacttt gacatgctcc tcttctttac tctgatagct
      961 tgactatgaa aattccgtca ccagcccctg ggttcgcaaa gataattgca ctgtttcttc
     1021 cttgaactct caagcctaca ggacacacat tcatcgtagg tataaacctc gaaaatcatt
     1081 cctactaaga tgggtataca atagtaacca tgcatggttg cctagtgaat gctccgtaac
     1141 acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta cccagaatgc
     1201 acaggtacac ttgtttagag gtaatccttc tttctagtgg gatctgacag acgggcaatt
     1261 gattacggga tcccattggt aacgaaatgt aaaagctagg agatcgtccg ccgatgtcag
     1321 gatgatttca cttgtttctt gtccggctca ccggtcaaag ctaaagagga gcaaaaggaa
     1381 cggatagaat cgggtgccgc tgatctatac ggtatagtgc ccttatcacg ttgactcaac
     1441 ccatgctatt taactcaacc cctccttctg aaccccacca tcttcttcct tttcctctca
     1501 tcccacacaa ttctctatct cagatttgaa ttccaaaagt cctcggacga aactgaacaa
     1561 gtcttcctcc cttcgataaa cctttggtga ttggaataac tgaccatctt ctatagttcc
     1621 caaaccaacc gacaatgtaa atacactcct cgattagccc tctagagggc atacgatgga
     1681 agtcatggaa tacttttggc tggactctca caatgatcaa ggtatcttag gtaacgtctt
     1741 tggcgtgggc cggtgttcgt tcccagtcat cgatgcattc acatgccctc cctaagctgg
     1801 gccctagact ctaggatcct agtctagaag gacatggcat cgatggactg ggttcgttct
     1861 gagattatac ggctaaaact tgatctggat aataccagcg aaaagggtca tgccttctct
     1921 cgttcttcct gttgatggaa tggctaacag atgatagtca ttgcaacttg aaacatgtct
     1981 cctccagctg ccatctacga acccactgtg gccgctaccg gcctcaaggg taaggtcgtg
     2041 gtttctgaga ccgtccccgt tgagggagct tctcagacca agctgttgga ccatttcggt
     2101 ggcaagtggg acgagttcaa gttcgcccct atccgcgaaa gccaggtctc tcgtgccatg
     2161 accagacgtt actttgagga cctggacaag tacgctgaaa gtgacgttgt cattgttggt
     2221 gctggttcct gcggtctgag cactgcgtac gtcttggcca aggctcgtcc ggacctgaag
     2281 attgctatcg tcgaggccag cgtctctcct ggtcagtagt ccatgatgga ttgccttgca
     2341 ctcagctttc cggaactaac gtgcaatagg tggcggtgcc tggttgggtg gccaactctt
     2401 ttctgctatg gtcatgcgcc gtcccgcgga agtcttcctg aacgagctgg gtgttcctta
     2461 cgaagaggac gcaaacccca actacgttgt cgtcaagcac gcctccctgt ttacctcgac
     2521 actcatgtcg aaggttctct ccttccccaa tgtcaagctc ttcaatgcta ccgctgttga
     2581 ggacttgatc acccgtccga ccgagaacgg caacccccag attgctggtg ttgtcgtcaa
     2641 ctggacgctg gtcacccttc accacgatga tcactcctgc atggacccca acactatcaa
     2701 cgctcctgtc atcatcagta ccactggtca cgatgggcca ttcggcgcct tctgtgcgaa
     2761 gcgcttggtg tccatgggca gcgtcgacaa gctaggtggc atgcgtggtc tcgacatgag
     2821 ctcggccgag gatgccatcg tcaagaacac ccgcgaggtt actaagggct tgataatcgg
     2881 cggtatggag ctgtctgaaa ttgatggctt taaccgcatg ggccctacct tcggtgccat
     2941 ggttctcagt ggtgtcaagg ctgccgagga ggcattgaag gtgttcgacg agcgtcagcg
     3001 cgagtgtgct gagtaaatga ctcactaccc gaatgggttc agtgcatgaa ccggatttgt
     3061 cttacggtct ttgacgatag gggaatgatg attatgtgat agttctgaga tttgaatgaa
     3121 ctcgttagct cgtaatccac atgcatatgt aaatggctgt gtcccgtatg taacggtggg
     3181 gcattctaga ataattatgt gtaacaagaa agacagtata atacaaacaa agatgcaaga
     3241 gcggctcatc gtcaccccat gatagactag agcttggcac tggccgtcgt tttacaacgt
     3301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3421 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3481 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagcca
     3541 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3601 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3661 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3721 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3841 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3901 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3961 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4021 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4081 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4141 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4201 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4261 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4321 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4381 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4441 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4501 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4561 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4621 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4681 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4741 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4801 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5041 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5701 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5761 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5821 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5881 ggaaacagct atgaccatga ttacggttac acccagcccc gtattgagac cctgctgaac
     5941 gagactgagc accacaaccg tcgccacgtc ctctacgctg gtcggccccc aagcgcctcc
     6001 gtcgcgactg gtctcaagtc cgtccacgcc aaggaggaac aggacttcct tcaggaagca
     6061 ttcactgtcc accagcatcg tctgaagggc gagtaagtgg ggacgttgta gccactgttg
     6121 gattatcgtc ttaggtgctt tgatttgtag gagctagcgt ctatccattt ctgtctcctt
     6181 tgtatgaact cactctgttg tcagcatcta ctgcggcttc cttcctacaa tcatactgtc
     6241 ctggtttttt gatatctttt tttttatttt tatttttttt tgaatgttcc ctggcatcct
     6301 attaggggtt gggattgact tgtctactcc tataacgtgc cggagcatgt gtgtgtaaac
     6361 taagaaggcc tgatttacta gatctgcatt attccttttt ccgtgatcat atgctgtcct
     6421 agagagatac atagttatcc agcaccgatg acgtcaaggg caaccaattg ggtaatatga
     6481 gtaagcgatt tagaacacct gaaatgcaag gcatcaatca cctgatgcgg gcccaaaggg
     6541 gaaagtcttg cttctccgca ctgcagaact tccgtctcct cttgcctcat tatttgacat
     6601 actatcccat caacaccttt ccccatgttg ttgagctcaa gaagtatccc agacttttgt
     6661 ttgtatcctt tcttctcatc tgattgagcg gtctctgacc tgttgcgttt gaacttcctc
     6721 ttcaacaatg gcatccgttg tttcgtcgtc ggccgccaac gctcagggtc aaaatgctcg
     6781 ccagtcagcg cgccagacac gtaccaatcc atccagaacg tccaagacgc tggggaggtc
     6841 atcgtttgcc tacggtcaca gttcaatgat cgaagcgcct cctacacctc ctgcccccca
     6901 cggattctat cctgcgctca ctcatttcac tgacgctata accgctctgc cgcgagaatt
     6961 tcgccgtcac aattcgttgc tgaaggaagt ggatgcaaag gcttgggcgc ttgaggataa
     7021 cctcctccaa ttgttgaagg tctcctcaga gtcgcaacct cttccttgtc cgccgaatcc
     7081 agcgcctatt gttgacgggg tggttcggga tgatattgtg tcagtggtag gtggtagata
     7141 tccataacaa tcatcacgct tgtctaaatc tgtgtgcttt acaatagaat ccctcgcaac
     7201 ctcctgaatc gcaagaaagc aaaaaccgtc gtctcctctt cgatcgcgtg cggcgcaccc
     7261 tagtggatct gatgatgacc gccgacgaaa agaaccatgt catttctaac gccaatgacg
     7321 aactagaccg gcagcttctg cgcatcgatg gcatatttcc tttcattgca ggggagatca
     7381 gtgaggaggc gcgactgggc agtcttacac actgggccta ttcaaaccgg aacgccacga
     7441 aaactgctac aaatgagcgc ccccgccgcg aagtcgcatc caacaaacaa gacctgaacc
     7501 atgctctgga aaacgaagct ggatcccgta gtgatgcgag acgggacgcc gctcgcaaac
     7561 agcggcgcac gcatgccgat tcggactttg acgatgctcg tgcatctggt tcgcgaaagg
     7621 ggcaggctag caaaccccgc agtggtgcag gtgcggctac tggggatcta tctgcagttg
     7681 ggcatggtgc acaaacttcg ggggcatcgg gggcatcggg tgcgacgaaa cgccgcaagg
     7741 tcgaaagacc tccagcggcc gatgcgggcg cagcgatgga aagatccgcc agcggcgctg
     7801 gaaatgcttc gggacgtggt gcatcgaagg acacgacagc ttcagatgcc accaagaaga
     7861 gatctcgcgc tcctaacacg aatgcagccg ctcgcaagag gtgagctttt tcttgggtta
     7921 tgcaattcta acttgtgcct ttgctaacca actggcagaa ataatacggg aacctccgcg
     7981 gcgaattcgc cagtgctggc acctactcct ctggctgggg cgtcgggtgc cgtgcgtagc
     8041 gcagccagtc ccggacctgg agcagccgct cggccccagt cttctcgtgc tcaacagagc
     8101 tctggacagc cgaccaatgg tcgccagcgg ccatcctcct cggcgtccaa tcgcctgaca
     8161 agcaacagta tgtcacctct ccattttctt tttgtctttc ttttgctaac cttaatcttg
     8221 attcaggcaa agtcgttgat accaagactc tacctaaaga aactccgaaa gcggatatgg
     8281 ttgcagctgc acttaactca gaactgcaac gccaagccga ggaagagaac tccggcacag
     8341 ccacagccaa ggctgtcgga cccgtcagca tgaaacgcga agatacagaa acccgccctg
     8401 cggagtcggt cgagtctcgc gaaacaccag tacctcaagc ttcgaacgca gccccgaagg
     8461 gccggtcgtc gaagacgtcc acgcctgtgc tctccacctt gtcagaaccc aactcgcagc
     8521 gcacccgacc tacccgcaac acagactccg cgcctgcgaa acgatcacat aagaagagtg
     8581 gcagtgtagc ggtatcccag cagcgagcag tgtctgagga ggaggactct ctgcatgaag
     8641 gggacgacga ggacgaagat ggcgagccgc ggtattgtta ctgcaacgag attagcttcg
     8701 gcgagatggt tgcttgtgac aacgatgcat gcccgcggga atggttccat ctgtcttgtg
     8761 ttgggttgac caaaccgcca gggaagaatg gtaagtttca ttggtcgaat gagtcggacc
     8821 atgctgatag ggcacagtca aatggtactg caatgaatgc aaggagaata tgcgaagaaa
     8881 tcggaacgcg cggtaaccag tcagagctcg gggtttcgta tgctcaggca cagttttgtt
     8941 ggttgggatg gtctcttggt ggcagtggcg gaggcagcgg cggtagtaaa ggagaagaac
     9001 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     9061 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     9121 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcacctatg
     9181 gtgttcaatg cttttcaaga tacccagatc atatgaagcg gcacgacttc
//
