LOCUS       plasmid                 9230 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     gene            complement(3465..5472)
                     /note="pLannotate"
                     /label="ptrA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="gene"
     promoter        complement(2852..2956)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1991..2851)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(6257..6970)
                     /note="pLannotate"
                     /label="mgfp5"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(5488..6216)
                     /note="pLannotate"
                     /label="trpC terminator"
                     /database="snapgene"
                     /identity="97.4"
                     /match_length="95.7"
                     /fragment="False"
                     /other="terminator"
     rep_origin      complement(1232..1820)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(878..908)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(923..944)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(854..870)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(3293..3447)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    3188..3287
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(7148..7291)
                     /note="pLannotate"
                     /label="YNG1 (fragment)"
                     /database="swissprot"
                     /identity="56.3"
                     /match_length="21.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(957..1049)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3051..3086
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tgaagaggaa gttcaaacgc aacaggtcag agaccgctca atcagatgag aagaaaggat
       61 acaaacaaaa gtctgggata cttcttgagc tcaacaacat ggggaaaggt gttgatggga
      121 tagtatgtca aataatgagg caagaggaga cggaagttct gcagtgcgga gaagcaagac
      181 tttccccttt gggcccgcat caggtgattg atgccttgca tttcaggtgt tctaaatcgc
      241 ttactcatat tacccaattg gttgcccttg acgtcatcgg tgctggataa ctatgtatct
      301 ctctaggaca gcatatgatc acggaaaaag gaataatgca gatctagtaa atcaggcctt
      361 cttagtttac acacacatgc tccggcacgt tataggagta gacaagtcaa tcccaacccc
      421 taataggatg ccagggaaca ttcaaaaaaa aataaaaata aaaaaaaaga tatcaaaaaa
      481 ccaggacagt atgattgtag gaaggaagcc gcagtagatg ctgacaacag agtgagttca
      541 tacaaaggag acagaaatgg atagacgcta gctcctacaa atcaaagcac ctaagacgat
      601 aatccaacag tggctacaac gtccccactt actcgccctt cagacgatgc tggtggacag
      661 tgaatgcttc ctgaaggaag tcctgttcct ccttggcgtg gacggacttg agaccagtcg
      721 cgacggaggc gcttgggggc cgaccagcgt agaggacgtg gcgacggttg tggtgctcag
      781 tctcgttcag cagggtctca atacggggct gggtgtaacc gtaatcatgg tcatagctgt
      841 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      901 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1021 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1141 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2581 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3001 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     3061 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3121 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3181 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3241 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     3301 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     3361 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     3421 cacgacgttg taaaacgacg gccagtgcca agctctagtc tatcatgggg tgacgatgag
     3481 ccgctcttgc atctttgttt gtattatact gtctttcttg ttacacataa ttattctaga
     3541 atgccccacc gttacatacg ggacacagcc atttacatat gcatgtggat tacgagctaa
     3601 cgagttcatt caaatctcag aactatcaca taatcatcat tcccctatcg tcaaagaccg
     3661 taagacaaat ccggttcatg cactgaaccc attcgggtag tgagtcattt actcagcaca
     3721 ctcgcgctga cgctcgtcga acaccttcaa tgcctcctcg gcagccttga caccactgag
     3781 aaccatggca ccgaaggtag ggcccatgcg gttaaagcca tcaatttcag acagctccat
     3841 accgccgatt atcaagccct tagtaacctc gcgggtgttc ttgacgatgg catcctcggc
     3901 cgagctcatg tcgagaccac gcatgccacc tagcttgtcg acgctgccca tggacaccaa
     3961 gcgcttcgca cagaaggcgc cgaatggccc atcgtgacca gtggtactga tgatgacagg
     4021 agcgttgata gtgttggggt ccatgcagga gtgatcatcg tggtgaaggg tgaccagcgt
     4081 ccagttgacg acaacaccag caatctgggg gttgccgttc tcggtcggac gggtgatcaa
     4141 gtcctcaaca gcggtagcat tgaagagctt gacattgggg aaggagagaa ccttcgacat
     4201 gagtgtcgag gtaaacaggg aggcgtgctt gacgacaacg tagttggggt ttgcgtcctc
     4261 ttcgtaagga acacccagct cgttcaggaa gacttccgcg ggacggcgca tgaccatagc
     4321 agaaaagagt tggccaccca accaggcacc gccacctatt gcacgttagt tccggaaagc
     4381 tgagtgcaag gcaatccatc atggactact gaccaggaga gacgctggcc tcgacgatag
     4441 caatcttcag gtccggacga gccttggcca agacgtacgc agtgctcaga ccgcaggaac
     4501 cagcaccaac aatgacaacg tcactttcag cgtacttgtc caggtcctca aagtaacgtc
     4561 tggtcatggc acgagagacc tggctttcgc ggataggggc gaacttgaac tcgtcccact
     4621 tgccaccgaa atggtccaac agcttggtct gagaagctcc ctcaacgggg acggtctcag
     4681 aaaccacgac cttacccttg aggccggtag cggccacagt gggttcgtag atggcagctg
     4741 gaggagacat gtttcaagtt gcaatgacta tcatctgtta gccattccat caacaggaag
     4801 aacgagagaa ggcatgaccc ttttcgctgg tattatccag atcaagtttt agccgtataa
     4861 tctcagaacg aacccagtcc atcgatgcca tgtccttcta gactaggatc ctagagtcta
     4921 gggcccagct tagggagggc atgtgaatgc atcgatgact gggaacgaac accggcccac
     4981 gccaaagacg ttacctaaga taccttgatc attgtgagag tccagccaaa agtattccat
     5041 gacttccatc gtatgccctc tagagggcta atcgaggagt gtatttacat tgtcggttgg
     5101 tttgggaact atagaagatg gtcagttatt ccaatcacca aaggtttatc gaagggagga
     5161 agacttgttc agtttcgtcc gaggactttt ggaattcaaa tctgagatag agaattgtgt
     5221 gggatgagag gaaaaggaag aagatggtgg ggttcagaag gaggggttga gttaaatagc
     5281 atgggttgag tcaacgtgat aagggcacta taccgtatag atcagcggca cccgattcta
     5341 tccgttcctt ttgctcctct ttagctttga ccggtgagcc ggacaagaaa caagtgaaat
     5401 catcctgaca tcggcggacg atctcctagc ttttacattt cgttaccaat gggatcccgt
     5461 aatcaattgc ccgtctgtca gatcccacta gaaagaagga ttacctctaa acaagtgtac
     5521 ctgtgcattc tgggtaaacg actcatagga gagttgtaaa aaagtttcgg ccggcgtatt
     5581 gggtgttacg gagcattcac taggcaacca tgcatggtta ctattgtata cccatcttag
     5641 taggaatgat tttcgaggtt tatacctacg atgaatgtgt gtcctgtagg cttgagagtt
     5701 caaggaagaa acagtgcaat tatctttgcg aacccagggg ctggtgacgg aattttcata
     5761 gtcaagctat cagagtaaag aagaggagca tgtcaaagta caattagaga caaatatata
     5821 gtcgcgtgga gccaagagcg gattcctcag tctcgtaggt ctcttgacga ccgttgatct
     5881 gcttgatctc gtctcccgaa aatgaaaata gactctgcta agctattctt ctgcttcgcc
     5941 ggagcctgaa gggcgtacta gggttgcgag gtccaatgca ttaatgcatt gcagatgagc
     6001 tgtatctgga agaggtaaac ccgaaacgcg ttttattctt gttgacatgg agctattaaa
     6061 tcactagaag gcactctttg ctgcttggac aaatgaacgt atcttatcga gatcctgaac
     6121 accatttgtc tcaactccgg agctgacatc gacaccaacg atcttatatc cagattcgtc
     6181 aagctgtttg atgatttcag taacgttaag tggatctcaa gcttgcggcc gcagagcatt
     6241 gtttgaggcg accggtttat ttgtatagtt catccatgcc atgtgtaatc ccagcagctg
     6301 ttacaaactc aagaaggacc atgtggtctc tcttttcgtt gggatctttc gaaagggcag
     6361 attgtgtgga caggtaatgg ttgtctggta aaaggacagg gccatcgcca attggagtat
     6421 tttgttgata atgatcagcg agttgcacgc cgccgtcttc gatgttgtgg cgggtcttga
     6481 agttggcttt gatgccgttc ttttgcttgt cggccatgat gtatacgttg tgggagttgt
     6541 agttgtattc caacttgtgg ccgaggatgt ttccgtcctc cttgaaatcg attcccttaa
     6601 gctcgatcct gttgacgagg gtgtctccct caaacttgac ttcagcacgt gtcttgtagt
     6661 tcccgtcgtc tttgaagaag atggtcctct cctgcacgta tccctcaggc atggcgctct
     6721 tgaagaagtc gtgccgcttc atatgatctg ggtatcttga aaagcattga acaccatagg
     6781 tgaaagtagt gacaagtgtt ggccatggaa caggtagttt tccagtagtg caaataaatt
     6841 taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca gaaaatttgt
     6901 gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg aaaagttctt
     6961 ctcctttact accgccgctg cctccgccac tgccaccaag agaccatccc aaccaacaaa
     7021 actgtgcctg agcatacgaa accccgagct ctgactggtt accgcgcgtt ccgatttctt
     7081 cgcatattct ccttgcattc attgcagtac catttgactg tgccctatca gcatggtccg
     7141 actcattcga ccaatgaaac ttaccattct tccctggcgg tttggtcaac ccaacacaag
     7201 acagatggaa ccattcccgc gggcatgcat cgttgtcaca agcaaccatc tcgccgaagc
     7261 taatctcgtt gcagtaacaa taccgcggct cgccatcttc gtcctcgtcg tccccttcat
     7321 gcagagagtc ctcctcctca gacactgctc gctgctggga taccgctaca ctgccactct
     7381 tcttatgtga tcgtttcgca ggcgcggagt ctgtgttgcg ggtaggtcgg gtgcgctgcg
     7441 agttgggttc tgacaaggtg gagagcacag gcgtggacgt cttcgacgac cggcccttcg
     7501 gggctgcgtt cgaagcttga ggtactggtg tttcgcgaga ctcgaccgac tccgcagggc
     7561 gggtttctgt atcttcgcgt ttcatgctga cgggtccgac agccttggct gtggctgtgc
     7621 cggagttctc ttcctcggct tggcgttgca gttctgagtt aagtgcagct gcaaccatat
     7681 ccgctttcgg agtttcttta ggtagagtct tggtatcaac gactttgcct gaatcaagat
     7741 taaggttagc aaaagaaaga caaaaagaaa atggagaggt gacatactgt tgcttgtcag
     7801 gcgattggac gccgaggagg atggccgctg gcgaccattg gtcggctgtc cagagctctg
     7861 ttgagcacga gaagactggg gccgagcggc tgctccaggt ccgggactgg ctgcgctacg
     7921 cacggcaccc gacgccccag ccagaggagt aggtgccagc actggcgaat tcgccgcgga
     7981 ggttcccgta ttatttctgc cagttggtta gcaaaggcac aagttagaat tgcataaccc
     8041 aagaaaaagc tcacctcttg cgagcggctg cattcgtgtt aggagcgcga gatctcttct
     8101 tggtggcatc tgaagctgtc gtgtccttcg atgcaccacg tcccgaagca tttccagcgc
     8161 cgctggcgga tctttccatc gctgcgcccg catcggccgc tggaggtctt tcgaccttgc
     8221 ggcgtttcgt cgcacccgat gcccccgatg cccccgaagt ttgtgcacca tgcccaactg
     8281 cagatagatc cccagtagcc gcacctgcac cactgcgggg tttgctagcc tgcccctttc
     8341 gcgaaccaga tgcacgagca tcgtcaaagt ccgaatcggc atgcgtgcgc cgctgtttgc
     8401 gagcggcgtc ccgtctcgca tcactacggg atccagcttc gttttccaga gcatggttca
     8461 ggtcttgttt gttggatgcg acttcgcggc gggggcgctc atttgtagca gttttcgtgg
     8521 cgttccggtt tgaataggcc cagtgtgtaa gactgcccag tcgcgcctcc tcactgatct
     8581 cccctgcaat gaaaggaaat atgccatcga tgcgcagaag ctgccggtct agttcgtcat
     8641 tggcgttaga aatgacatgg ttcttttcgt cggcggtcat catcagatcc actagggtgc
     8701 gccgcacgcg atcgaagagg agacgacggt ttttgctttc ttgcgattca ggaggttgcg
     8761 agggattcta ttgtaaagca cacagattta gacaagcgtg atgattgtta tggatatcta
     8821 ccacctacca ctgacacaat atcatcccga accaccccgt caacaatagg cgctggattc
     8881 ggcggacaag gaagaggttg cgactctgag gagaccttca acaattggag gaggttatcc
     8941 tcaagcgccc aagcctttgc atccacttcc ttcagcaacg aattgtgacg gcgaaattct
     9001 cgcggcagag cggttatagc gtcagtgaaa tgagtgagcg caggatagaa tccgtggggg
     9061 gcaggaggtg taggaggcgc ttcgatcatt gaactgtgac cgtaggcaaa cgatgacctc
     9121 cccagcgtct tggacgttct ggatggattg gtacgtgtct ggcgcgctga ctggcgagca
     9181 ttttgaccct gagcgttggc ggccgacgac gaaacaacgg atgccattgt
//
