LOCUS       plasmid                 9230 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(8074..9115)
                     /note="pLannotate"
                     /label="ptrA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(7217..7321)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(6356..7216)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1392..2105)
                     /note="pLannotate"
                     /label="mgfp5"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(623..1351)
                     /note="pLannotate"
                     /label="trpC terminator"
                     /database="snapgene"
                     /identity="97.4"
                     /match_length="95.7"
                     /fragment="False"
                     /other="terminator"
     rep_origin      complement(5597..6185)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(5243..5273)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5288..5309)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     gene            complement(1..607)
                     /note="pLannotate"
                     /label="ptrA (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="30.2"
                     /fragment="True"
                     /other="gene"
     protein_bind    complement(5219..5235)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(7658..7812)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    7553..7652
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2283..2426)
                     /note="pLannotate"
                     /label="YNG1 (fragment)"
                     /database="swissprot"
                     /identity="56.3"
                     /match_length="21.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5322..5414)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             7416..7451
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gaacgaaccc agtccatcga tgccatgtcc ttctagacta ggatcctaga gtctagggcc
       61 cagcttaggg agggcatgtg aatgcatcga tgactgggaa cgaacaccgg cccacgccaa
      121 agacgttacc taagatacct tgatcattgt gagagtccag ccaaaagtat tccatgactt
      181 ccatcgtatg ccctctagag ggctaatcga ggagtgtatt tacattgtcg gttggtttgg
      241 gaactataga agatggtcag ttattccaat caccaaaggt ttatcgaagg gaggaagact
      301 tgttcagttt cgtccgagga cttttggaat tcaaatctga gatagagaat tgtgtgggat
      361 gagaggaaaa ggaagaagat ggtggggttc agaaggaggg gttgagttaa atagcatggg
      421 ttgagtcaac gtgataaggg cactataccg tatagatcag cggcacccga ttctatccgt
      481 tccttttgct cctctttagc tttgaccggt gagccggaca agaaacaagt gaaatcatcc
      541 tgacatcggc ggacgatctc ctagctttta catttcgtta ccaatgggat cccgtaatca
      601 attgcccgtc tgtcagatcc cactagaaag aaggattacc tctaaacaag tgtacctgtg
      661 cattctgggt aaacgactca taggagagtt gtaaaaaagt ttcggccggc gtattgggtg
      721 ttacggagca ttcactaggc aaccatgcat ggttactatt gtatacccat cttagtagga
      781 atgattttcg aggtttatac ctacgatgaa tgtgtgtcct gtaggcttga gagttcaagg
      841 aagaaacagt gcaattatct ttgcgaaccc aggggctggt gacggaattt tcatagtcaa
      901 gctatcagag taaagaagag gagcatgtca aagtacaatt agagacaaat atatagtcgc
      961 gtggagccaa gagcggattc ctcagtctcg taggtctctt gacgaccgtt gatctgcttg
     1021 atctcgtctc ccgaaaatga aaatagactc tgctaagcta ttcttctgct tcgccggagc
     1081 ctgaagggcg tactagggtt gcgaggtcca atgcattaat gcattgcaga tgagctgtat
     1141 ctggaagagg taaacccgaa acgcgtttta ttcttgttga catggagcta ttaaatcact
     1201 agaaggcact ctttgctgct tggacaaatg aacgtatctt atcgagatcc tgaacaccat
     1261 ttgtctcaac tccggagctg acatcgacac caacgatctt atatccagat tcgtcaagct
     1321 gtttgatgat ttcagtaacg ttaagtggat ctcaagcttg cggccgcaga gcattgtttg
     1381 aggcgaccgg tttatttgta tagttcatcc atgccatgtg taatcccagc agctgttaca
     1441 aactcaagaa ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt
     1501 gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
     1561 tgataatgat cagcgagttg cacgccgccg tcttcgatgt tgtggcgggt cttgaagttg
     1621 gctttgatgc cgttcttttg cttgtcggcc atgatgtata cgttgtggga gttgtagttg
     1681 tattccaact tgtggccgag gatgtttccg tcctccttga aatcgattcc cttaagctcg
     1741 atcctgttga cgagggtgtc tccctcaaac ttgacttcag cacgtgtctt gtagttcccg
     1801 tcgtctttga agaagatggt cctctcctgc acgtatccct caggcatggc gctcttgaag
     1861 aagtcgtgcc gcttcatatg atctgggtat cttgaaaagc attgaacacc ataggtgaaa
     1921 gtagtgacaa gtgttggcca tggaacaggt agttttccag tagtgcaaat aaatttaagg
     1981 gtaagttttc cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca
     2041 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
     2101 ttactaccgc cgctgcctcc gccactgcca ccaagagacc atcccaacca acaaaactgt
     2161 gcctgagcat acgaaacccc gagctctgac tggttaccgc gcgttccgat ttcttcgcat
     2221 attctccttg cattcattgc agtaccattt gactgtgccc tatcagcatg gtccgactca
     2281 ttcgaccaat gaaacttacc attcttccct ggcggtttgg tcaacccaac acaagacaga
     2341 tggaaccatt cccgcgggca tgcatcgttg tcacaagcaa ccatctcgcc gaagctaatc
     2401 tcgttgcagt aacaataccg cggctcgcca tcttcgtcct cgtcgtcccc ttcatgcaga
     2461 gagtcctcct cctcagacac tgctcgctgc tgggataccg ctacactgcc actcttctta
     2521 tgtgatcgtt tcgcaggcgc ggagtctgtg ttgcgggtag gtcgggtgcg ctgcgagttg
     2581 ggttctgaca aggtggagag cacaggcgtg gacgtcttcg acgaccggcc cttcggggct
     2641 gcgttcgaag cttgaggtac tggtgtttcg cgagactcga ccgactccgc agggcgggtt
     2701 tctgtatctt cgcgtttcat gctgacgggt ccgacagcct tggctgtggc tgtgccggag
     2761 ttctcttcct cggcttggcg ttgcagttct gagttaagtg cagctgcaac catatccgct
     2821 ttcggagttt ctttaggtag agtcttggta tcaacgactt tgcctgaatc aagattaagg
     2881 ttagcaaaag aaagacaaaa agaaaatgga gaggtgacat actgttgctt gtcaggcgat
     2941 tggacgccga ggaggatggc cgctggcgac cattggtcgg ctgtccagag ctctgttgag
     3001 cacgagaaga ctggggccga gcggctgctc caggtccggg actggctgcg ctacgcacgg
     3061 cacccgacgc cccagccaga ggagtaggtg ccagcactgg cgaattcgcc gcggaggttc
     3121 ccgtattatt tctgccagtt ggttagcaaa ggcacaagtt agaattgcat aacccaagaa
     3181 aaagctcacc tcttgcgagc ggctgcattc gtgttaggag cgcgagatct cttcttggtg
     3241 gcatctgaag ctgtcgtgtc cttcgatgca ccacgtcccg aagcatttcc agcgccgctg
     3301 gcggatcttt ccatcgctgc gcccgcatcg gccgctggag gtctttcgac cttgcggcgt
     3361 ttcgtcgcac ccgatgcccc cgatgccccc gaagtttgtg caccatgccc aactgcagat
     3421 agatccccag tagccgcacc tgcaccactg cggggtttgc tagcctgccc ctttcgcgaa
     3481 ccagatgcac gagcatcgtc aaagtccgaa tcggcatgcg tgcgccgctg tttgcgagcg
     3541 gcgtcccgtc tcgcatcact acgggatcca gcttcgtttt ccagagcatg gttcaggtct
     3601 tgtttgttgg atgcgacttc gcggcggggg cgctcatttg tagcagtttt cgtggcgttc
     3661 cggtttgaat aggcccagtg tgtaagactg cccagtcgcg cctcctcact gatctcccct
     3721 gcaatgaaag gaaatatgcc atcgatgcgc agaagctgcc ggtctagttc gtcattggcg
     3781 ttagaaatga catggttctt ttcgtcggcg gtcatcatca gatccactag ggtgcgccgc
     3841 acgcgatcga agaggagacg acggtttttg ctttcttgcg attcaggagg ttgcgaggga
     3901 ttctattgta aagcacacag atttagacaa gcgtgatgat tgttatggat atctaccacc
     3961 taccactgac acaatatcat cccgaaccac cccgtcaaca ataggcgctg gattcggcgg
     4021 acaaggaaga ggttgcgact ctgaggagac cttcaacaat tggaggaggt tatcctcaag
     4081 cgcccaagcc tttgcatcca cttccttcag caacgaattg tgacggcgaa attctcgcgg
     4141 cagagcggtt atagcgtcag tgaaatgagt gagcgcagga tagaatccgt ggggggcagg
     4201 aggtgtagga ggcgcttcga tcattgaact gtgaccgtag gcaaacgatg acctccccag
     4261 cgtcttggac gttctggatg gattggtacg tgtctggcgc gctgactggc gagcattttg
     4321 accctgagcg ttggcggccg acgacgaaac aacggatgcc attgttgaag aggaagttca
     4381 aacgcaacag gtcagagacc gctcaatcag atgagaagaa aggatacaaa caaaagtctg
     4441 ggatacttct tgagctcaac aacatgggga aaggtgttga tgggatagta tgtcaaataa
     4501 tgaggcaaga ggagacggaa gttctgcagt gcggagaagc aagactttcc cctttgggcc
     4561 cgcatcaggt gattgatgcc ttgcatttca ggtgttctaa atcgcttact catattaccc
     4621 aattggttgc ccttgacgtc atcggtgctg gataactatg tatctctcta ggacagcata
     4681 tgatcacgga aaaaggaata atgcagatct agtaaatcag gccttcttag tttacacaca
     4741 catgctccgg cacgttatag gagtagacaa gtcaatccca acccctaata ggatgccagg
     4801 gaacattcaa aaaaaaataa aaataaaaaa aaagatatca aaaaaccagg acagtatgat
     4861 tgtaggaagg aagccgcagt agatgctgac aacagagtga gttcatacaa aggagacaga
     4921 aatggataga cgctagctcc tacaaatcaa agcacctaag acgataatcc aacagtggct
     4981 acaacgtccc cacttactcg cccttcagac gatgctggtg gacagtgaat gcttcctgaa
     5041 ggaagtcctg ttcctccttg gcgtggacgg acttgagacc agtcgcgacg gaggcgcttg
     5101 ggggccgacc agcgtagagg acgtggcgac ggttgtggtg ctcagtctcg ttcagcaggg
     5161 tctcaatacg gggctgggtg taaccgtaat catggtcata gctgtttcct gtgtgaaatt
     5221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5521 ataacgcagg aaagaacatg ttagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7321 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     7381 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     7441 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7501 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     7561 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7621 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     7681 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     7741 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     7801 cgacggccag tgccaagctc tagtctatca tggggtgacg atgagccgct cttgcatctt
     7861 tgtttgtatt atactgtctt tcttgttaca cataattatt ctagaatgcc ccaccgttac
     7921 atacgggaca cagccattta catatgcatg ttgattacga gctaacgagt tcattcaaat
     7981 ctcagaacta tcacataatc atcattcccc tatcgtcaaa gaccgtaaga caaatccggt
     8041 tcatgcactg aacccattcg ggtagtgagt catttactca gcacactcgc gctgacgctc
     8101 gtcgaacacc ttcaatgcct cctcggcagc cttgacacca ctgagaacca tggcaccgaa
     8161 ggtagggccc atgcggttaa agccatcaat ttcagacagc tccataccgc cgattatcaa
     8221 gcccttagta acctcgcggg tgttcttgac gatggcatcc tcggccgagc tcatgtcgag
     8281 accacgcatg ccacctagct tgtcgacgct gcccatggac accaagcgct tcgcacagaa
     8341 ggcgccgaat ggcccatcgt gaccagtggt actgatgatg acaggagcgt tgatagtgtt
     8401 ggggtccatg caggagtgat catcgtggtg aagggtgacc agcgtccagt tgacgacaac
     8461 accagcaatc tgggggttgc cgttctcggt cggacgggtg atcaagtcct caacagcggt
     8521 agcattgaag agcttgacat tggggaagga gagaaccttc gacatgagtg tcgaggtaaa
     8581 cagggaggcg tgcttgacga caacgtagtt ggggtttgcg tcctcttcgt aaggaacacc
     8641 cagctcgttc aggaagactt ccgcgggacg gcgcatgacc atagcagaaa agagttggcc
     8701 acccaaccag gcaccgccac ctattgcacg ttagttccgg aaagctgagt gcaaggcaat
     8761 ccatcatgga ctactgacca ggagagacgc tggcctcgac gatagcaatc ttcaggtccg
     8821 gacgagcctt ggccaagacg tacgcagtgc tcagaccgca ggaaccagca ccaacaatga
     8881 caacgtcact ttcagcgtac ttgtccaggt cctcaaagta acgtctggtc atggcacgag
     8941 agacctggct ttcgcggata ggggcgaact tgaactcgtc ccacttgcca ccgaaatggt
     9001 ccaacagctt ggtctgagaa gctccctcaa cggggacggt ctcagaaacc acgaccttac
     9061 ccttgaggcc ggtagcggcc acagtgggtt cgtagatggc agctggagga gacatgtttc
     9121 aagttgcaat gactatcatc tgttagccat tccatcaaca ggaagaacga gagaaggcat
     9181 gacccttttc gctggtatta tccagatcaa gttttagccg tataatctca
//
