LOCUS       plasmid                 9230 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             495..1536
                     /note="pLannotate"
                     /label="ptrA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        2289..2393
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             2394..3254
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7505..8218
                     /note="pLannotate"
                     /label="mgfp5"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     terminator      8259..8987
                     /note="pLannotate"
                     /label="trpC terminator"
                     /database="snapgene"
                     /identity="97.4"
                     /match_length="95.7"
                     /fragment="False"
                     /other="terminator"
     rep_origin      3425..4013
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4337..4367
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4301..4322
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    4375..4391
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1798..1952
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1958..2057)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.9"
                     /fragment="True"
                     /other="misc_feature"
     gene            9003..9230
                     /note="pLannotate"
                     /label="ptrA (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="11.4"
                     /fragment="True"
                     /other="gene"
     ncRNA           348..442
                     /note="pLannotate"
                     /label="TPP (fragment)"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="90.5"
                     /fragment="True"
                     /other="ncRNA"
     CDS             7184..7327
                     /note="pLannotate"
                     /label="YNG1 (fragment)"
                     /database="swissprot"
                     /identity="56.3"
                     /match_length="21.9"
                     /fragment="True"
                     /other="CDS"
     CDS             4196..4288
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2159..2194)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcttcttcct tttcctctca tcccacacaa ttctctatct cagatttgaa ttccaaaagt
       61 cctcggacga aactgaacaa gtcttcctcc cttcgataaa cctttggtga ttggaataac
      121 tgaccatctt ctatagttcc caaaccaacc gacaatgtaa atacactcct cgattagccc
      181 tctagagggc atacgatgga agtcatggaa tacttttggc tggactctca caatgatcaa
      241 ggtatcttag gtaacgtctt tggcgtgggc cggtgttcgt tcccagtcat cgatgcattc
      301 acatgccctc cctaagctgg gccctagact ctaggatcct agtctagaag gacatggcat
      361 cgatggactg ggttcgttct gagattatac ggctaaaact tgatctggat aataccagcg
      421 aaaagggtca tgccttctct cgttcttcct gttgatggaa tggctaacag atgatagtca
      481 ttgcaacttg aaacatgtct cctccagctg ccatctacga acccactgtg gccgctaccg
      541 gcctcaaggg taaggtcgtg gtttctgaga ccgtccccgt tgagggagct tctcagacca
      601 agctgttgga ccatttcggt ggcaagtggg acgagttcaa gttcgcccct atccgcgaaa
      661 gccaggtctc tcgtgccatg accagacgtt actttgagga cctggacaag tacgctgaaa
      721 gtgacgttgt cattgttggt gctggttcct gcggtctgag cactgcgtac gtcttggcca
      781 aggctcgtcc ggacctgaag attgctatcg tcgaggccag cgtctctcct ggtcagtagt
      841 ccatgatgga ttgccttgca ctcagctttc cggaactaac gtgcaatagg tggcggtgcc
      901 tggttgggtg gccaactctt ttctgctatg gtcatgcgcc gtcccgcgga agtcttcctg
      961 aacgagctgg gtgttcctta cgaagaggac gcaaacccca actacgttgt cgtcaagcac
     1021 gcctccctgt ttacctcgac actcatgtcg aaggttctct ccttccccaa tgtcaagctc
     1081 ttcaatgcta ccgctgttga ggacttgatc acccgtccga ccgagaacgg caacccccag
     1141 attgctggtg ttgtcgtcaa ctggacgctg gtcacccttc accacgatga tcactcctgc
     1201 atggacccca acactatcaa cgctcctgtc atcatcagta ccactggtca cgatgggcca
     1261 ttcggcgcct tctgtgcgaa gcgcttggtg tccatgggca gcgtcgacaa gctaggtggc
     1321 atgcgtggtc tcgacatgag ctcggccgag gatgccatcg tcaagaacac ccgcgaggtt
     1381 actaagggct tgataatcgg cggtatggag ctgtctgaaa ttgatggctt taaccgcatg
     1441 ggccctacct tcggtgccat ggttctcagt ggtgtcaagg ctgccgagga ggcattgaag
     1501 gtgttcgacg agcgtcagcg cgagtgtgct gagtaaatga ctcactaccc gaatgggttc
     1561 agtgcatgaa ccggatttgt cttacggtct ttgacgatag gggaattatg attatgtgat
     1621 agttctgaga tttgaatgaa ctcgttagct cgtaatccac atgcatatgt aaatggctgt
     1681 gtcccgtatg taacggtggg gcattctaga ataattatgt gtaacaagaa agacagtata
     1741 atacaaacaa agatgcaaga gcggctcatc gtcaccccat gatagactag agcttggcac
     1801 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     1861 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     1921 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     1981 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     2041 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     2101 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     2161 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     2221 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     2401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     3061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     3121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     3181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     3241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     3301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     3361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4201 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4261 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4321 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4381 gcggataaca atttcacaca ggaaacagct atgaccatga ttacggttac acccagcccc
     4441 gtattgagac cctgctgaac gagactgagc accacaaccg tcgccacgtc ctctacgctg
     4501 gtcggccccc aagcgcctcc gtcgcgactg gtctcaagtc cgtccacgcc aaggaggaac
     4561 aggacttcct tcaggaagca ttcactgtcc accagcatcg tctgaagggc gagtaagtgg
     4621 ggacgttgta gccactgttg gattatcgtc ttaggtgctt tgatttgtag gagctagcgt
     4681 ctatccattt ctgtctcctt tgtatgaact cactctgttg tcagcatcta ctgcggcttc
     4741 cttcctacaa tcatactgtc ctggtttttt gatatctttt tttttatttt tatttttttt
     4801 tgaatgttcc ctggcatcct attaggggtt gggattgact tgtctactcc tataacgtgc
     4861 cggagcatgt gtgtgtaaac taagaaggcc tgatttacta gatctgcatt attccttttt
     4921 ccgtgatcat atgctgtcct agagagatac atagttatcc agcaccgatg acgtcaaggg
     4981 caaccaattg ggtaatatga gtaagcgatt tagaacacct gaaatgcaag gcatcaatca
     5041 cctgatgcgg gcccaaaggg gaaagtcttg cttctccgca ctgcagaact tccgtctcct
     5101 cttgcctcat tatttgacat actatcccat caacaccttt ccccatgttg ttgagctcaa
     5161 gaagtatccc agacttttgt ttgtatcctt tcttctcatc tgattgagcg gtctctgacc
     5221 tgttgcgttt gaacttcctc ttcaacaatg gcatccgttg tttcgtcgtc ggccgccaac
     5281 gctcagggtc aaaatgctcg ccagtcagcg cgccagacac gtaccaatcc atccagaacg
     5341 tccaagacgc tggggaggtc atcgtttgcc tacggtcaca gttcaatgat cgaagcgcct
     5401 cctacacctc ctgcccccca cggattctat cctgcgctca ctcatttcac tgacgctata
     5461 accgctctgc cgcgagaatt tcgccgtcac aattcgttgc tgaaggaagt ggatgcaaag
     5521 gcttgggcgc ttgaggataa cctcctccaa ttgttgaagg tctcctcaga gtcgcaacct
     5581 cttccttgtc cgccgaatcc agcgcctatt gttgacgggg tggttcggga tgatattgtg
     5641 tcagtggtag gtggtagata tccataacaa tcatcacgct tgtctaaatc tgtgtgcttt
     5701 acaatagaat ccctcgcaac ctcctgaatc gcaagaaagc aaaaaccgtc gtctcctctt
     5761 cgatcgcgtg cggcgcaccc tagtggatct gatgatgacc gccgacgaaa agaaccatgt
     5821 catttctaac gccaatgacg aactagaccg gcagcttctg cgcatcgatg gcatatttcc
     5881 tttcattgca ggggagatca gtgaggaggc gcgactgggc agtcttacac actgggccta
     5941 ttcaaaccgg aacgccacga aaactgctac aaatgagcgc ccccgccgcg aagtcgcatc
     6001 caacaaacaa gacctgaacc atgctctgga aaacgaagct ggatcccgta gtgatgcgag
     6061 acgggacgcc gctcgcaaac agcggcgcac gcatgccgat tcggactttg acgatgctcg
     6121 tgcatctggt tcgcgaaagg ggcaggctag caaaccccgc agtggtgcag gtgcggctac
     6181 tggggatcta tctgcagttg ggcatggtgc acaaacttcg ggggcatcgg gggcatcggg
     6241 tgcgacgaaa cgccgcaagg tcgaaagacc tccagcggcc gatgcgggcg cagcgatgga
     6301 aagatccgcc agcggcgctg gaaatgcttc gggacgtggt gcatcgaagg acacgacagc
     6361 ttcagatgcc accaagaaga gatctcgcgc tcctaacacg aatgcagccg ctcgcaagag
     6421 gtgagctttt tcttgggtta tgcaattcta acttgtgcct ttgctaacca actggcagaa
     6481 ataatacggg aacctccgcg gcgaattcgc cagtgctggc acctactcct ctggctgggg
     6541 cgtcgggtgc cgtgcgtagc gcagccagtc ccggacctgg agcagccgct cggccccagt
     6601 cttctcgtgc tcaacagagc tctggacagc cgaccaatgg tcgccagcgg ccatcctcct
     6661 cggcgtccaa tcgcctgaca agcaacagta tgtcacctct ccattttctt tttgtctttc
     6721 ttttgctaac cttaatcttg attcaggcaa agtcgttgat accaagactc tacctaaaga
     6781 aactccgaaa gcggatatgg ttgcagctgc acttaactca gaactgcaac gccaagccga
     6841 ggaagagaac tccggcacag ccacagccaa ggctgtcgga cccgtcagca tgaaacgcga
     6901 agatacagaa acccgccctg cggagtcggt cgagtctcgc gaaacaccag tacctcaagc
     6961 ttcgaacgca gccccgaagg gccggtcgtc gaagacgtcc acgcctgtgc tctccacctt
     7021 gtcagaaccc aactcgcagc gcacccgacc tacccgcaac acagactccg cgcctgcgaa
     7081 acgatcacat aagaagagtg gcagtgtagc ggtatcccag cagcgagcag tgtctgagga
     7141 ggaggactct ctgcatgaag gggacgacga ggacgaagat ggcgagccgc ggtattgtta
     7201 ctgcaacgag attagcttcg gcgagatggt tgcttgtgac aacgatgcat gcccgcggga
     7261 atggttccat ctgtcttgtg ttgggttgac caaaccgcca gggaagaatg gtaagtttca
     7321 ttggtcgaat gagtcggacc atgctgatag ggcacagtca aatggtactg caatgaatgc
     7381 aaggagaata tgcgaagaaa tcggaacgcg cggtaaccag tcagagctcg gggtttcgta
     7441 tgctcaggca cagttttgtt ggttgggatg gtctcttggt ggcagtggcg gaggcagcgg
     7501 cggtagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     7561 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     7621 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     7681 tgtcactact ttcacctatg gtgttcaatg cttttcaaga tacccagatc atatgaagcg
     7741 gcacgacttc ttcaagagcg ccatgcctga gggatacgtg caggagagga ccatcttctt
     7801 caaagacgac gggaactaca agacacgtgc tgaagtcaag tttgagggag acaccctcgt
     7861 caacaggatc gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa
     7921 gttggaatac aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg
     7981 catcaaagcc aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga
     8041 tcattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     8101 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     8161 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataaac
     8221 cggtcgcctc aaacaatgct ctgcggccgc aagcttgaga tccacttaac gttactgaaa
     8281 tcatcaaaca gcttgacgaa tctggatata agatcgttgg tgtcgatgtc agctccggag
     8341 ttgagacaaa tggtgttcag gatctcgata agatacgttc atttgtccaa gcagcaaaga
     8401 gtgccttcta gtgatttaat agctccatgt caacaagaat aaaacgcgtt tcgggtttac
     8461 ctcttccaga tacagctcat ctgcaatgca ttaatgcatt ggacctcgca accctagtac
     8521 gcccttcagg ctccggcgaa gcagaagaat agcttagcag agtctatttt cattttcggg
     8581 agacgagatc aagcagatca acggtcgtca agagacctac gagactgagg aatccgctct
     8641 tggctccacg cgactatata tttgtctcta attgtacttt gacatgctcc tcttctttac
     8701 tctgatagct tgactatgaa aattccgtca ccagcccctg ggttcgcaaa gataattgca
     8761 ctgtttcttc cttgaactct caagcctaca ggacacacat tcatcgtagg tataaacctc
     8821 gaaaatcatt cctactaaga tgggtataca atagtaacca tgcatggttg cctagtgaat
     8881 gctccgtaac acccaatacg ccggccgaaa cttttttaca actctcctat gagtcgttta
     8941 cccagaatgc acaggtacac ttgtttagag gtaatccttc tttctagtgg gatctgacag
     9001 acgggcaatt gattacggga tcccattggt aacgaaatgt aaaagctagg agatcgtccg
     9061 ccgatgtcag gatgatttca cttgtttctt gtccggctca ccggtcaaag ctaaagagga
     9121 gcaaaaggaa cggatagaat cgggtgccgc tgatctatac ggtatagtgc ccttatcacg
     9181 ttgactcaac ccatgctatt taactcaacc cctccttctg aaccccacca
//
