LOCUS       plasmid                 8778 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(7635..8354)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(395..894)
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      3792..4220
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(1031..1271)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(1990..2223)
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     LTR             complement(2894..3074)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(2722..2847)
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(1346..1463)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    complement(6577..6688)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4273..4377
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4378..5238
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(8433..8778,1..359))
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="99.1"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
     rep_origin      5409..5997
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(7040..7626)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="99.7"
                     /fragment="False"
                     /other="misc_feature"
     enhancer        complement(3292..3592)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.0"
                     /fragment="False"
                     /other="enhancer"
     protein_bind    6285..6306
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(3092..3291)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3606..3624)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        6420..6438
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             complement(6714..6892)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.9"
                     /fragment="False"
                     /other="LTR"
     protein_bind    6359..6375
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             3632..3787
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2400..2738)
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="22.6"
                     /fragment="True"
                     /other="CDS"
     misc_RNA        complement(917..984)
                     /note="pLannotate"
                     /label="tracrRNA (fragment)"
                     /database="snapgene"
                     /identity="98.5"
                     /match_length="86.1"
                     /fragment="True"
                     /other="misc_RNA"
     promoter        6321..6351
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="96.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1513..1613)
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6927..7046)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="92.5"
                     /match_length="19.8"
                     /fragment="True"
                     /other="CDS"
     CDS             6180..6272
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1003..1022)
                     /note="pLannotate"
                     /label="VN155(I152L) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ccgtcctgca gagaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
       61 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
      121 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
      181 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      241 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
      301 accttgaagc gcaggaactc cttgatgatg gcggagttat cctcctcgcc cttgctcacg
      361 ctcatggtgg ctcgagatct agatggatgc aggtcgaaag gcccggagat gaggaagagg
      421 agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc
      481 ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc
      541 ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc
      601 ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta
      661 cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta
      721 ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac
      781 cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg
      841 gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga
      901 attggatcca aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc
      961 ttatttaaac ttgctatgct gtttccagca tagctcttaa acgggtgtcg ccctcgaact
     1021 tcggtgtttc gtcctttcca caagatatat aaagccaaga aatcgaaata ctttcaagtt
     1081 acggtaagca tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga
     1141 aattattact ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta
     1201 attctaatta tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga
     1261 aataggccct cttcctgccc gaccttggac gcgtagggcc cggtactaac caaactggat
     1321 ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
     1381 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
     1441 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctg cagaattggc
     1501 gcacgcagtg ccgatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
     1561 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
     1621 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     1681 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     1741 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     1801 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     1861 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     1921 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     1981 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     2041 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     2101 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     2161 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     2221 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     2281 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     2341 ctcatatctc ctcctccagg tctgaagatc agcgcggccg gccgcttgct gtgcggtggt
     2401 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     2461 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     2521 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     2581 ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     2641 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     2701 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     2761 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     2821 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     2881 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     2941 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     3001 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     3061 aaccagagag acccagtaca ggcaaaacgc gctgcttata tagacctccc accgtacacg
     3121 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     3181 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     3241 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     3301 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     3361 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     3421 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     3481 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     3541 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg tactagtcaa
     3601 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     3661 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3721 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3781 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4021 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4081 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4141 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4201 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     4261 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4321 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5221 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5521 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6061 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6181 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6241 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6301 actcattagg caccccaggc tttacacttt atgctcccgg ctcgtatgtt gtgtggaatt
     6361 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     6421 attaaccctc actaaaggga acaaaagctt aattaacggc cgcccataga gcccaccgca
     6481 tccccagcat gcctgctatt gacttcccaa tcctccccct tgctgtcctg ccccacccca
     6541 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     6601 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     6661 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta aactgctaga
     6721 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     6781 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     6841 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag actctggtct
     6901 aaccagagag acccagtaca ggcaaaaagc agatcttgac ttctttggga gtgaattagc
     6961 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     7021 attggtctta aaggtacctg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     7081 cgaaggcgta gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     7141 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     7201 acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca
     7261 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     7321 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
     7381 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
     7441 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
     7501 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
     7561 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
     7621 ggttgagcgg ccgcttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     7681 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     7741 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     7801 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     7861 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     7921 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     7981 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     8041 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     8101 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     8161 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     8221 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     8281 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     8341 cccttgctca ccatgaattc tgggccagga ttctcctcca cgtcaccgca tgttagaaga
     8401 gatcctctgc cctctcctcc ggacccgccg cccttgtaca gctcgtccat gccgccggtg
     8461 gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag
     8521 gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc
     8581 ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc
     8641 ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag
     8701 cccattgtct tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc
     8761 accttgtaga tgaactcg
//
