LOCUS       plasmid                 7806 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(11..685)
                     /note="pLannotate"
                     /label="TRP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      7259..7687
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(686..833)
                     /note="pLannotate"
                     /label="TRP1 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3499..6420)
                     /note="pLannotate"
                     /label="CDC15"
                     /database="swissprot"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        1253..1357
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1358..2218
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2389..2977
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        3301..3331
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3265..3286
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(7074..7092)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3400..3418
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3339..3355
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             7100..7255
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    7002..7065
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.3"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(7715..7766)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    3431..3475
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="41.7"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(972..1021)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3160..3252
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1123..1158)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     rep_origin      complement(7771..7806)
                     /note="pLannotate"
                     /label="ARS1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="7.9"
                     /fragment="True"
                     /other="rep_origin"
ORIGIN
        1 cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta gagtctttta
       61 caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt aatctaagcg
      121 catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag ctttcggggc
      181 tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca cctgtcccac
      241 ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct gcactgagta
      301 gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg aggaactctt
      361 ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg taatcattga
      421 ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag tatttcggag
      481 tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca ataaccgggt
      541 caattgttct ctttctattg ggcacacata taatacccag caagtcagca tcggaatcta
      601 gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat ggaccagaac
      661 tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta atcacgtata
      721 ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt cttttttcga
      781 ccgaattaat tcttaatcgg caaaaaaaga aaagctccgg atcaagattg tacgtaaggt
      841 gacaagctat ttttcaataa agaatatctt ccactactgc catctggcgt cataactgca
      901 aagtacacat atattacgat gctgttctat taaatgcttc ctatattata tatatagtaa
      961 tgtcgttgat ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1021 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1081 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     1141 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1201 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     3181 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     3241 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     3301 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     3361 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     3421 acaaaagctg gagctccacc gcggtggcgg ccgctctaga actagtggat cccccatgca
     3481 catgcaattc ctacattatg tttgaaatgt tgtaatgggg acggaaaagc cgtcactttt
     3541 atctttggag gatcgcaaat tactacgctc atcttttgtt ggagaactac caattgctgc
     3601 agtgacgctt gaaacttttt gcaggcttcc tttttttgat aatgaacgga tatcctcaca
     3661 tagctgacag atcaaaactg agtccccccc aacttgattt gaattccttt ttgggacatc
     3721 cttgttccag ttgttgttta aaaactccgt tatttgtact aaaatggcac gaagtttgaa
     3781 aacagtggcc ttattacgac gatttggttc ggtttgcttt ataggatccg gctgtcctgt
     3841 gtagtcatat agcttcaata acgattttga gaatattagc tttatgaatt tcaatagatc
     3901 gatttgaata agtaaactgt tgaaataggt atcaaagaaa gaaacaatct tttcataaaa
     3961 gttcgggtga aatatgatat taattgtaag gttttcaaaa ccgggcaatg agcatatctt
     4021 cgtgaaaatg ctaattagtt tactgaggtt aacgtcatcg tcattcaatg cataaaacag
     4081 gtcaactaat tgatcaatcg gtaattcaat agcagcgtca ttgttgacct ttatgaggaa
     4141 aacactatga gactctgatg agcccactgt aggagccaca tcatcattta cattgcgtaa
     4201 cgtaaaatgc aagcagttca gtatgatctc cagtccactt atggaggtgt tattcttttt
     4261 tctgatgaaa ttcatagctg tagaaaataa agaagcactc atttggtcca tatccaaact
     4321 catttcaacg catatagcag tgatttgttt ccaaatgaaa gctgctgtct ttgcgtcgtc
     4381 gataaaggga atgatggtat caatattccg gataaaagca aaaaaagcat cgttgtcaag
     4441 cagatcattt aaaaaagtgc tgtttatatg tactgtaagg taacaaagct tggtgaagta
     4501 ctttaaaaca gataaatctt tgatttgggc catgtcaaca aaaaaagatg ttagccaggt
     4561 aaagaaaccc tccggtaatt caattagtag tcttgatttt gaagatatgg tcagatgggg
     4621 aaagtttgaa gtgctcttgt gtcgcattgg agaagaggga cgcgttgcca tcaaagaatg
     4681 aacaggggac cttgatggag actgtaccga attcactggt gagttcctcg tgggtgagga
     4741 ggataacggt agagaggaag aagaaggaat agcggacttg tgtattttat cgtcattcgt
     4801 ggttatcata tagtttattg atttgaagac tacgtaagta atttgaggac tgattaaaat
     4861 tttctttttt agcttagagt caattaaaga gggcaaaatt ttctcaaaag accatggtgc
     4921 atatgacgat agctttagta gtatggattg ggctcttctt tcatggatgt tattcagaag
     4981 gagtgatata tcgaggtgtt tgaaacacca gcgacaccag aaggctgtgg atgttaaatc
     5041 gtagaaccta tagacgagtt ctaaaatata ctttggggtt ttcagcgatg caaaattcgg
     5101 aggatacatt attccacatt caattaaagt ctgagggtag tcgatgacga actctttggc
     5161 taaatgttcg aatttaatga tcagtggaat tcctcccata gcgatgaatt tcagtcgcaa
     5221 cctagagtgg ttatgctggg tatcgtaaac aaaaatggag ccaaatgcag ttattaatcg
     5281 tttatcaaca gttgtgcgcg acagacactc gataattgta tcagcgatgt tctcgaggga
     5341 gcaaacactg aaaagcacat gcaagtccgt caaaggctta gatgaactct tcaattggct
     5401 cagcaattga ctttcagtgg ggggcattaa atctagttct tgattgtttt ctgcccaggc
     5461 agcgggagcc gctgcaagac tgaatttaga gggtgatata tttagtttct cttcttgaaa
     5521 atcggcatcc caatgataat cagcgtcggt aaagtcctcc ttgaacttgt tgagcttgtc
     5581 gaccttcaca ttttcggtag agttgatcca cacatgcttg agtaactggt cggctgtcgg
     5641 cctcttgtac atgtttttca caaagcattt agataagaaa tcctttagtg gctcagagaa
     5701 agagctaggt gggtagtagg tatcattttc aacagcgtag tagatattgg cgtctgtcaa
     5761 attgtggtag ggtggattct ttgtgagcat ttcaactaca gtggcaccta gagaccaaat
     5821 gtcgctgagc gtagaagctc ccctgttgcc caggatctct ggagccatcc aattgagtgt
     5881 gcccgctagc gttaaggcgc tggagttcac aatagtggaa acgccaaaat cagcaagttt
     5941 gacagtgtta tcagcactca gcaggatgtt agccgccttg atgtccctgt ggatgactcc
     6001 ttcaccgtgt aaatatttca gccccaatag tgtctgtgtc acataggttt tcgattcatt
     6061 ttcacttaat ccggtagagc tccttgaaat gagcctcctc aaagaaccat tagcgcagta
     6121 ttcgaggagg atatacaatt catagctttt tcgtatgaag ccgtggtatt taacaatatt
     6181 gttatggttt aaatttttta acaagctaat ttctgccata atgtcattaa gttcctcatc
     6241 attttcgtac acgacctcct ttattgccac gacttggtca gtatgtttat taatggcttt
     6301 gtaaactacc ccgtaagaac ccctcccaat gacctgcttc aagtggtatt gcacggattt
     6361 ctcagatgcc ctctggatgg gagtcaagtt gactctatcg gtatcggcca tactgttcat
     6421 ggtatagtct taccaggaaa atgggtagtg cttatgtgtg ttttgtcctt cctcgagcct
     6481 ccaagtagaa gatatacctt ttgtgaggca gatctcccgt atacaaaaat aacagcaaga
     6541 aaagcggaaa gaccatcgca aggtggaaag gattataatg gcacagcaaa gtccgcacag
     6601 agcactacag tatagcatag agtgctaatg agttgatagg cccaattttg attatgcctc
     6661 ttttccatac acgacgccag aggacattat tacattacag tagttcgccg ctagatgaca
     6721 aacgacatcc ttaccgatat gagatgtgca aagctacata atggcaacaa gcgatatgaa
     6781 cagccttgtc tttacgacca cagaaaagcc gtattagagc tcttcagctg caaaattttc
     6841 ttctaatatg atgcaaagcc atcaaaaatc atgcatagtt atgaaatacc tgatgaaacg
     6901 cttcgagttc gtgctcaaga aattactgaa aggttaccga gaagaaaaat atctatgaga
     6961 cacgataagg ccccttctga acccattgtc ctgggcttgt tcgggctgca ggaattcgat
     7021 atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccaatt cgccctatag
     7081 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     7141 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     7201 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     7261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7321 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7441 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7561 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7621 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7681 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     7741 atctgtgcgg tatttcacac cgcatagatc ggcaagtgca caaacaatac ttaaataaat
     7801 actact
//
