LOCUS       plasmid                 7690 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(3688..6609)
                     /note="pLannotate"
                     /label="CDC15"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(200..874)
                     /note="pLannotate"
                     /label="TRP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(875..1022)
                     /note="pLannotate"
                     /label="TRP1 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1442..1546
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1547..2407
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2578..3166
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        3490..3520
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3454..3475
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(7263..7281)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        3589..3607
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3528..3544
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      7448..7690
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="67.7"
                     /fragment="True"
                     /other="rep_origin"
     CDS             7289..7444
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    7191..7254
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.3"
                     /fragment="True"
                     /other="misc_feature"
     rep_origin      1..97
                     /note="pLannotate"
                     /label="M13 ori (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="21.5"
                     /fragment="True"
                     /other="rep_origin"
     misc_feature    complement(98..149)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    3620..3664
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="41.7"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(1161..1210)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             3349..3441
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1312..1347)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
       61 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
      121 cgcatctgtg cggtatttca caccgcatag atcggcaagt gcacaaacaa tacttaaata
      181 aatactactc agtaataacc tatttcttag catttttgac gaaatttgct attttgttag
      241 agtcttttac accatttgtc tccacacctc cgcttacatc aacaccaata acgccattta
      301 atctaagcgc atcaccaaca ttttctggcg tcagtccacc agctaacata aaatgtaagc
      361 tttcggggct ctcttgcctt ccaacccagt cagaaatcga gttccaatcc aaaagttcac
      421 ctgtcccacc tgcttctgaa tcaaacaagg gaataaacga atgaggtttc tgtgaagctg
      481 cactgagtag tatgttgcag tcttttggaa atacgagtct tttaataact ggcaaaccga
      541 ggaactcttg gtattcttgc cacgactcat ctccatgcag ttggacgata tcaatgccgt
      601 aatcattgac cagagccaaa acatcctcct taggttgatt acgaaacacg ccaaccaagt
      661 atttcggagt gcctgaacta tttttatatg cttttacaag acttgaaatt ttccttgcaa
      721 taaccgggtc aattgttctc tttctattgg gcacacatat aatacccagc aagtcagcat
      781 cggaatctag agcacattct gcggcctctg tgctctgcaa gccgcaaact ttcaccaatg
      841 gaccagaact acctgtgaaa ttaataacag acatactcca agctgccttt gtgtgcttaa
      901 tcacgtatac tcacgtgctc aatagtcacc aatgccctcc ctcttggccc tctccttttc
      961 ttttttcgac cgaattaatt cttaatcggc aaaaaaagaa aagctccgga tcaagattgt
     1021 acgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc atctggcgtc
     1081 ataactgcaa agtacacata tattacgatg ctgttctatt aaatgcttcc tatattatat
     1141 atatagtaat gtcgttgatc tatggtgcac tctcagtaca atctgctctg atgccgcata
     1201 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     1261 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     1321 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     1381 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1441 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1501 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1561 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1621 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1681 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1741 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1801 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1861 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1921 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1981 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2041 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2101 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2161 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2221 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2281 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2341 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2401 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2461 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2521 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2581 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2641 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2701 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2761 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2821 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2881 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2941 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3001 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3061 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3121 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3181 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3241 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3301 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     3361 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     3421 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     3481 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     3541 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     3601 ctaaagggaa caaaagctgg agctccaccg cggtggcggc cgctctagaa ctagtggatc
     3661 ccccatgcac atgcaattcc tacattatgt ttgaaatgtt gtaatgggga cggaaaagcc
     3721 gtcactttta tctttggagg atcgcaaatt actacgctca tcttttgttg gagaactacc
     3781 aattgctgca gtgacgcttg aaactttttg caggcttcct ttttttgata atgaacggat
     3841 atcctcacat agctgacaga tcaaaactga gtccccccca acttgatttg aattcctttt
     3901 tgggacatcc ttgttccagt tgttgtttaa aaactccgtt atttgtacta aaatggcacg
     3961 aagtttgaaa acagtggcct tattacgacg atttggttcg gtttgcttta taggatccgg
     4021 ctgtcctgtg tagtcatata gcttcaataa cgattttgag aatattagct ttatgaattt
     4081 caatagatcg atttgaataa gtaaactgtt gaaataggta tcaaagaaag aaacaatctt
     4141 ttcataaaag ttcgggtgaa atatgatatt aattgtaagg ttttcaaaac cgggcaatga
     4201 gcatatcttc gtgaaaatgc taattagttt actgaggtta acgtcatcgt cattcaatgc
     4261 ataaaacagg tcaactaatt gatcaatcgg taattcaata gcagcgtcat tgttgacctt
     4321 tatgaggaaa acactatgag actctgatga gcccactgta ggagccacat catcatttac
     4381 attgcgtaac gtaaaatgca agcagttcag tatgatctcc agtccactta tggaggtgtt
     4441 attctttttt ctgatgaaat tcatagctgt agaaaataaa gaagcactca tttggtccat
     4501 atccaaactc atttcaacgc atatagcagt gatttgtttc caaatgaaag ctgctgtctt
     4561 tgcgtcgtcg ataaagggaa tgatggtatc aatattccgg ataaaagcaa aaaaagcatc
     4621 gttgtcaagc agatcattta aaaaagtgct gtttatatgt actgtaaggt aacaaagctt
     4681 ggtgaagtac tttaaaacag ataaatcttt gatttgggcc atgtcaacaa aaaaagatgt
     4741 tagccaggta aagaaaccct ccggtaattc aattagtagt cttgattttg aagatatggt
     4801 cagatgggga aagtttgaaa tgctcttgtg tcgcattgga gaagagggac gcgttgccat
     4861 caaagaatga acaggggacc ttgatggaga ctgtaccgaa ttcactggtg agttcctcgt
     4921 gggtgaggag gataacggta gagaggaaga agaaggaata gcggacttgt gtattttatc
     4981 gtcattcgtg gttatcatat agtttattga tttgaagact acgtaagtaa tttgaggact
     5041 gattaaaatt ttctttttta gcttagagtc aattaaagag ggcaaaattt tctcaaaaga
     5101 ccatggtgca tatgacgata gctttagtag tatggattgg gctcttcttt catggatgtt
     5161 attcagaagg agtgatatat cgaggtgttt gaaacaccag cgacaccaga aggctgtgga
     5221 tgttaaatcg tagaacctat agacgagttc taaaatatac tttggggttt tcagcgatgc
     5281 aaaattcgga ggatacatta ttccacattc aattaaagtc tgagggtagt cgatgacgaa
     5341 ctctttggct aaatgttcga atttaatgat cagtggaatt cctcccatag cgatgaattt
     5401 cagtcgcaac ctagagtggt tatgctgggt atcgtaaaca aaaatggagc caaatgcagt
     5461 tattaatcgt ttatcaacag ttgtgcgcga cagacactcg ataattgtat cagcgatgtt
     5521 ctcgagggag caaacactga aaagcacatg caagtccgtc aaaggcttag atgaactctt
     5581 caattggctc agcaattgac tttcagtggg gggcattaaa tctagttctt gattgttttc
     5641 tgcccaggca gcgggagccg ctgcaagact gaatttagag ggtgatatat ttagtttctc
     5701 ttcttgaaaa tcggcatccc aatgataatc agcgtcggta aagtcctcct tgaacttgtt
     5761 gagcttgtcg accttcacat tttcggtaga gttgatccac acatgcttga gtaactggtc
     5821 ggctgtcggc ctcttgtaca tgtttttcac aaagcattta gataagaaat cctttagtgg
     5881 ctcagagaaa gagctaggtg ggtagtaggt atcattttca acagcgtagt agatattggc
     5941 gtctgtcaaa ttgtggtagg gtggattctt tgtgagcatt tcaactacag tggcacctag
     6001 agaccaaatg tcgctgagcg tagaagctcc cctgttgccc aggatctctg gagccatcca
     6061 attgagtgtg cccgctagcg ttaaggcgct ggagttcaca atagtggaaa cgccaaaatc
     6121 agcaagtttg acagtgttat cagcactcag caggatgtta gccgccttga tgtccctgtg
     6181 gatgactcct tcaccgtgta aatatttcag ccccaatagt gtctgtgtca cataggtttt
     6241 cgattcattt tcacttaatc cggtagagct ccttgaaatg agcctcctca aagaaccatt
     6301 agcgcagtat tcgaggagga tatacaattc atagcttttt cgtatgaagc cgtggtattt
     6361 aacaatattg ttatggttta aattttttaa caagctaatt tctgccataa tgtcattaag
     6421 ttcctcatca ttttcgtaca cgacctcctt tattgccacg acttggtcag tatgtttatt
     6481 aatggctttg taaactaccc cgtaagaacc cctcccaatg acctgcttca agtggtattg
     6541 cacggatttc tcagatgccc tctggatggg agtcaagttg actctatcgg tatcggccat
     6601 actgttcatg gtatagtctt accaggaaaa tgggtagtgc ttatgtgtgt tttgtccttc
     6661 ctcgagcctc caagtagaag atataccttt tgtgaggcag atctcccgta tacaaaaata
     6721 acagcaagaa aagcggaaag accatcgcaa ggtggaaagg attataatgg cacagcaaag
     6781 tccgcacaga gcactacagt atagcataga gtgctaatga gttgataggc ccaattttga
     6841 ttatgcctct tttccataca cgacgccaga ggacattatt acattacagt agttcgccgc
     6901 tagatgacaa acgacatcct taccgatatg agatgtgcaa agctacataa tggcaacaag
     6961 cgttatgaac agccttgtct ttacgaccac agaaaagccg tattagagct cttcagctgc
     7021 aaaattttct tctaatatga tgcaaagcca tcaaaaatca tgcatagtta tgaaatacct
     7081 gatgaaacgc ttcgagttcg tgctcaagaa attactgaaa ggttaccgag aagaaaaata
     7141 tctatgagac acgataaggc cccttctgaa tccattgtcc tgggcttgtt cgggctgcag
     7201 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc
     7261 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7321 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7381 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7441 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7501 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7561 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7621 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7681 agtgggccat
//
