LOCUS       plasmid                 6606 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(785..1459)
                     /note="pLannotate"
                     /label="TRP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      227..655
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(4595..6076)
                     /note="pLannotate"
                     /label="HRR25"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(1460..1607)
                     /note="pLannotate"
                     /label="TRP1 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2027..2131
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             2132..2992
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3163..3751
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        4075..4105
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4039..4060
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        4174..4192
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(42..60)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4113..4129
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             68..223
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    join(6577..6606,1..33)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.3"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(683..734)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    4205..4249
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="41.7"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(1746..1795)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             6124..6426
                     /note="pLannotate"
                     /label="YP205_YEAST (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="88.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3934..4026
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1897..1932)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gataccgtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta
       61 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
      241 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      301 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      421 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      541 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      601 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      661 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
      721 tttcacaccg catagatcgg caagtgcaca aacaatactt aaataaatac tactcagtaa
      781 taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat
      841 ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac
      901 caacattttc tggcgtcagt ccaccagcta acataaaatg taagctttcg gggctctctt
      961 gccttccaac ccagtcagaa atcgagttcc aatccaaaag ttcacctgtc ccacctgctt
     1021 ctgaatcaaa caagggaata aacgaatgag gtttctgtga agctgcactg agtagtatgt
     1081 tgcagtcttt tggaaatacg agtcttttaa taactggcaa accgaggaac tcttggtatt
     1141 cttgccacga ctcatctcca tgcagttgga cgatatcaat gccgtaatca ttgaccagag
     1201 ccaaaacatc ctccttaggt tgattacgaa acacgccaac caagtatttc ggagtgcctg
     1261 aactattttt atatgctttt acaagacttg aaattttcct tgcaataacc gggtcaattg
     1321 ttctctttct attgggcaca catataatac ccagcaagtc agcatcggaa tctagagcac
     1381 attctgcggc ctctgtgctc tgcaagccgc aaactttcac caatggacca gaactacctg
     1441 tgaaattaat aacagacata ctccaagctg cctttgtgtg cttaatcacg tatactcacg
     1501 tgctcaatag tcaccaatgc cctccctctt ggccctctcc ttttcttttt tcgaccgaat
     1561 taattcttaa tcggcaaaaa aagaaaagct ccggatcaag attgtacgta aggtgacaag
     1621 ctatttttca ataaagaata tcttccacta ctgccatctg gcgtcataac tgcaaagtac
     1681 acatatatta cgatgctgtt ctattaaatg cttcctatat tatatatata gtaatgtcgt
     1741 tgatctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     1801 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     1861 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     1921 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1981 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2341 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2641 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     2701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     2761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     2821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     2881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     2941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3301 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3781 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     3841 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     3901 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3961 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4021 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4081 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4141 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     4201 gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccc gaattgatgc
     4261 tgtcttctca gagggccctc ctttaaaacc gtccaatatt cgtgattggt cttcgatcgt
     4321 tagcccttta ttacataaaa aaaaacaaat tggccaagag gtgctccgct cccccttaat
     4381 ttccttcccg aagaaaaaaa tgcataaaaa aagtggttct tttagctatc atctttttag
     4441 gtaagaaggc cagaattgta tatattaact cgatatggaa ttgtgtgtac gtatatgtaa
     4501 ataataataa taatatatat atatatatgt gtgtgtgtat gtctatatat acatatgttt
     4561 atttttgtgc gttttgagca atatatgttg cttacaacca aattgactgg ccagctggtt
     4621 tatcttgagg cggctgttgt ggattagatc tcatttgtgg aggtacagat gcttgattag
     4681 tattaaaatt attgctgttt atttgtgggg gatactgagt agccctggtt gtagccatcg
     4741 gaacttgctg ctgttgctcc tgagaatctc tttgttgctg ctgtagtaac ggttcaggat
     4801 aataatttgg tctttggcca tttggttgct gctgtagctg ttggggctgt ggttgtggct
     4861 gcgaactttg gacctgctgc tgcggctgct gttgtctcaa gttttccata cctttatcta
     4921 gtgcgttcaa taattcctct ggtaaagaag aggctgcatt attattcaag atagtttgtt
     4981 gtttgatctc ttctggtgaa ggattatgtt tgtcttcatt cttgtaatag tggaaatggg
     5041 tggggaattt cttcatggct aacagtttaa tcttgttgaa agtttcagac ttgttgtctg
     5101 tgctgttact tgcacttgct gcattgctat ttgcgttcaa atcacctttt tcgatgagga
     5161 ggtccctttg cttctccacc atcgcctttg tgtaacgcaa cattgtccaa tcgaacaagt
     5221 ggtcgttgtg atactctagt ttaatactca gatctttaaa cagccttgcc aagaacaaat
     5281 aatctggctt ctcatcgaat ttcaaattct tacagtaagc catatattct tgaaactcta
     5341 atggtaaacc tgaacataga gtttccacgc taacgtttaa tttcttttcc atgatacgat
     5401 catacttttg tttcttggtg gttgctttca aaccctgcca tggcaaagaa cccttacaaa
     5461 aatagatcaa gacataacct agtgattcta agtcatctct tctactttgc tctattccaa
     5521 gatgcgtatt gacacttgca taacgagctg tacctgtcaa ggacttgttc tccctgtaag
     5581 gaatatgacg atgtgtgttg aaatctcggt atttctttga tagaccgaaa tcaataacat
     5641 gaacggtgct accacggcgt cctaccccca ttaaaaagtt gtctggtttg atatctctat
     5701 gaatgaacga ccttccatgt atatactgaa tacggcaaaa catttgcaaa gccagcatga
     5761 taaccgtctt aaaggagaac cttctgtgac agtagttgaa taaatcttcc aaagatgggc
     5821 ctagaagatc gatgaccata gcattatatt caccctctct gccaaaccat ctgatgaacg
     5881 ggattcccac accaccgctt aagtatctgt agacgcggga ctcatagtcc aattgaggat
     5941 gtctggacct gatcgattcc agcttgatgg ctacttcttc accactaatt aagttcgtgc
     6001 cgtggtaaat gtcaccaaag gaaccactcc caatcttcct gccaatacga aatttccttc
     6061 ctactcttaa gtccatctct ttttaatgta tcctttctat aaatatatct tttctttttg
     6121 gttttttttt tggtcgaata tgtcgtaact tttcgacttt ttttatcctt gaagtctgga
     6181 aatatttttc taagtaccca acagaggttt aaagatgcta ctaagatgtg ttttcaccac
     6241 caaatgcttt cctcgagtgt cgaacctttc ttctctttgt atttcaacca cgttccatat
     6301 atgtggtcgt gtttcaactt tttttcttcg tctccttttc ctcgttgcac caaaaattct
     6361 ccagaaaaaa agaaattcaa acgtgggctg cctattagta gtaaatacac agacgggaag
     6421 aagcgttgaa acaaagcgag agttgcagta actcttcaca gtggtatatg attgccacga
     6481 aataatcgtc gatccagtgg tagttattcg tatataggag ccttttatag caattagatg
     6541 gtatccattt tgtggtactg ttgcgggtaa tctgcagggc tgcaggaatt cgatatcaag
     6601 cttatc
//
