LOCUS       plasmid                 6890 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             5008..5682
                     /note="pLannotate"
                     /label="TRP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5812..6240)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             390..1871
                     /note="pLannotate"
                     /label="HRR25"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        4860..5007
                     /note="pLannotate"
                     /label="TRP1 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(4336..4440)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3475..4335)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2716..3304)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(2362..2392)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(2407..2428)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        6407..6425
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        6689..6707
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(2275..2293)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(2338..2354)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(6244..6399)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(6716..6778)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.3"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(6434..6496)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="58.3"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(6609..6681)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="42.0"
                     /fragment="True"
                     /other="CDS"
     misc_feature    5733..5784
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(2218..2262)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="41.7"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    4672..4721
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(39..380)
                     /note="pLannotate"
                     /label="YP205_YEAST"
                     /database="swissprot"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2441..2533)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             4535..4570
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ccactgtgaa gagttactgc aactctcgct ttgtttcaac gcttcttccc gtctgtgtat
       61 ttactactaa taggcagccc acgtttgaat ttcttttttt ctggagaatt tttggtgcaa
      121 cgaggaaaag gagacgaaga aaaaaagttg aaacacgacc acatatatgg aacgtggttg
      181 aaatacaaag agaagaaagg ttcgacactc gaggaaagca tttggtggtg aaaacacatc
      241 ttagtagcat ctttaaacct ctgttgggta cttagagaaa tatttccaga cttcaaggat
      301 aaaaaaagtc gaaaagttac gacatattcg accaaaaaaa aaaaccaaaa agaaaagata
      361 tatttataga aaggatacat taaaaagaga tggacttaag agtaggaagg aaatttcgta
      421 ttggcaggaa gattgggagt ggttcctttg gtgacattta ccacggcacg aacttaatta
      481 gtggtgaaga agtagccatc aagctggaat cgatcaggtc cagacatcct caattggact
      541 atgagtcccg cgtctacaga tacttaagcg gtggtgtggg aatcccgttc atcagatggt
      601 ttggcagaga gggtgaatat aatgctatgg tcatcgatct tctaggccca tctttggaag
      661 atttattcaa ctactgtcac agaaggttct cctttaagac ggttatcatg ctggctttgc
      721 aaatgttttg ccgtattcag tatatacatg gaaggtcgtt cattcataga gatatcaaac
      781 cagacaactt tttaatgggg gtaggacgcc gtggtagcac cgttcatgtt attgatttcg
      841 gtctatcaaa gaaataccga gatttcaaca cacatcgtca tattccttac agggagaaca
      901 agtccttgac aggtacagct cgttatgcaa gtgtcaatac gcatcttgga atagagcaaa
      961 gtagaagaga tgacttagaa tcactaggtt atgtcttgat ctatttttgt aagggttctt
     1021 tgccatggca gggtttgaaa gcaaccacca agaaacaaaa gtatgatcgt atcatggaaa
     1081 agaaattaaa cgttagcgtg gaaactctat gttcaggttt accattagag tttcaagaat
     1141 atatggctta ctgtaagaat ttgaaattcg atgagaagcc agattatttg ttcttggcaa
     1201 ggctgtttaa agatctgagt attaaactag agtatcacaa cgaccacttg ttcgattgga
     1261 caatgttgcg ttacacaaag gcgatggtgg agaagcaaag ggacctcctc atcgaaaaag
     1321 gtgatttgaa cgcaaatagc aatgcagcaa gtgcaagtaa cagcacagac aacaagtctg
     1381 aaactttcaa caagattaaa ctgttagcca tgaagaaatt ccccacccat ttccactatt
     1441 acaagaatga agacaaacat aatccttcac cagaagagat caaacaacaa actatcttga
     1501 ataataatgc agcctcttct ttaccagagg aattattgaa cgcactagat aaaggtatgg
     1561 aaaacttgag acaacagcag ccgcagcagc aggtccaaag ttcgcagcca caaccacagc
     1621 cccaacagct acagcagcaa ccaaatggcc aaagaccaaa ttattatcct gaaccgttac
     1681 tacagcagca acaaagagat tctcaggagc aacagcagca agttccgatg gctacaacca
     1741 gggctactca gtatccccca caaataaaca gcaataattt taatactaat caagcatctg
     1801 tacctccaca aatgagatct aatccacaac agccgcctca agataaacca gctggccagt
     1861 caatttggtt gtaagcaaca tatattgctc aaaacgcaca aaaataaaca tatgtatata
     1921 tagacataca cacacacata tatatatata tattattatt attatttaca tatacgtaca
     1981 cacaattcca tatcgagtta atatatacaa ttctggcctt cttacctaaa aagatgatag
     2041 ctaaaagaac cacttttttt atgcattttt tacttcggga aggaaattaa gggggagcgg
     2101 agcacctctt ggccaatttg tttttttttt atgtaataaa gggctaacga tcgaagatca
     2161 atcacgaata ttggacggtt ttaaaggagg gccctctgag aagacagcat caattcgggg
     2221 ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     2281 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4441 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4501 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     4561 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4621 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     4681 ctatgcggca tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata
     4741 tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta
     4801 tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg
     4861 tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa
     4921 agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg
     4981 attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt
     5041 ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc
     5101 gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt
     5161 attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa
     5221 tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat
     5281 tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc
     5341 ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt
     5401 gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca
     5461 ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa
     5521 agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga
     5581 ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac
     5641 tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat
     5701 ttatttaagt attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc
     5761 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     5821 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     5881 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5941 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     6001 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     6061 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     6121 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     6181 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     6241 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6301 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6361 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     6421 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
     6481 cgaattcctg cagccctgca gattacccgc aacagtacca caaaatggat accatctaat
     6541 tgctataaaa ggctcctata tacgaataac taccactgga tcgacgatta tttcgtggca
     6601 atcatatagt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6661 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     6721 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagccctg
     6781 cagattaccc gcaacagtac cacaaaatgg ataccatcta attgctataa aaggctccta
     6841 tatacgaata actaccactg gatcgacgat tatttcgtgg caatcatata
//
