LOCUS       plasmid                 6779 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     mobile_element  4493..4717
                     /note="pLannotate"
                     /label="Tn7R"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     mobile_element  853..1018
                     /note="pLannotate"
                     /label="Tn7L"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     polyA_signal    complement(1047..1181)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(85..189)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3252..3343)
                     /note="pLannotate"
                     /label="polyhedrin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(5782..6558)
                     /note="pLannotate"
                     /label="bla(M)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.7"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1347..2036)
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="96.6"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5022..5610)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3893..4426
                     /note="pLannotate"
                     /label="GmR"
                     /database="snapgene"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(215..669)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2058..3197)
                     /note="pLannotate"
                     /label="wc-2 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="71.7"
                     /fragment="True"
                     /other="CDS"
     promoter        3695..3723
                     /note="pLannotate"
                     /label="Pc promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(6558..6779,1..84))
                     /note="pLannotate"
                     /label="AmpR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4785..4836
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(2038..2057)
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3370..3717
                     /note="pLannotate"
                     /label="YPR2_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="94.8"
                     /match_length="88.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1204..1287)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(674..690)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
       61 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      121 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      181 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
      241 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      361 aacaagagtc cactattaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
      421 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
      481 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
      541 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
      601 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
      661 agggcgcgtc ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa
      721 acattaataa cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg
      781 aagatgacgg tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga
      841 aaaatgcccc gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt
      901 tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag
      961 ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg
     1021 gatctcctag gctcaagcag tgatcagatc cagacatgat aagatacatt gatgagtttg
     1081 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1141 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1201 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     1261 acaaatgtgg tatggctgat tatgatcctc tagtacttct cgacaagctt ggtaccgcat
     1321 gcctcgagtt agtggtgatg gtggtggtgg gtgatgccag cggctgtcac gaattccagc
     1381 aggaccatgt ggtctctctt ctcgttaggg tccttagaca gagcggactg tgtgctcagg
     1441 tagtggttgt caggcagcag cacggggccg tcgccgatag gggtgttctg ctggtagtga
     1501 tcggccagct gcacgctgcc atcttccaca ttgtgtctga tcttgaagtt ggccttgata
     1561 ccattcttct gcttgtcggc tgtgatgtac acgttgtggc tgttgaagtt gtattccagc
     1621 ttgtggccca ggatgttgcc atcttccttg aagtcgatgc ccttcagctc gattctgttc
     1681 accagtgtat ctccctcgaa cttcacctcg gctctggtct tataggtgcc atcgtcttta
     1741 aaagagatgg tccgctcctg cacgtagccc tcaggcatgg cggatttgaa gaaatcatgc
     1801 cgcttcatgt ggtcggggta tctgctaaaa cattgcacgc cgtatgtcag ggttgtgacc
     1861 agggtgggcc aaggcacagg cagtttgccg gtggtgcaga taaatttcag ggtcagcttt
     1921 ccgttggtgg cgtcgccttc gccctcgccc cgcacgctga acttgtgtcc attcacgtcg
     1981 ccgtcgagct caaccagaat tggcaccact ccggtgaaca gttcctcgcc cttgcttccc
     2041 tgaaaataca ggttttctcc catatgatcg cccatgggtg tatggatgtc gttgtgaccg
     2101 cctatacctc cgccgttatt gttgttgttg gcgttcttct ttttctcttt ctttgcccag
     2161 cgaagaccgc aggcattgca tagtgtcttg ggtccactag ggccttttcg ccattcgggg
     2221 gaatcgagcg taccgcagtc ggtgcacacg tattcctctg caaccttgat cttcttcttc
     2281 ttctctcccg tccggggatc tcggtctagg ggaatcgcga ttcccgcatc acccttaatg
     2341 agcgtcgggc tggcattacc cgttgtgata ccatgacttc tttcaccctc ctggtacttg
     2401 agtcctgtaa gcatttcaat cgtgtctgcg tgattgccct cgtaacgcaa catcttttcg
     2461 cgtatagaat ccggccggct tccggcaatg ccttccaggt tctcccgcgt cagcgcaatg
     2521 ttaagcgacg acgccggctg gctgggtggt ggcatcataa cgtcagcttg ggcattcatg
     2581 ggaatgtaga agggcgtcat tcccatctgg gtcgcggtat cgtcggacgg cgtaacatca
     2641 gatcgtccct cctggctcat tcgccatgtc ctatgcgact cttcctgctc ctcctgctcc
     2701 tctctgcgca gctcggcaat cctgcgcttc agtctttcgt tttcaatctt gtgttccaga
     2761 aaggaatcca acagacccgc gttcttggtc gggtagggtc gagccatcat gaacacggcc
     2821 tggcagaaag gcgactggtt ctgtggattg ggtgcaaact tggcggcggc gatgtgcgca
     2881 tgaccaacag tctcgaaaat ggtccagttt ccatcctttt tccggaaccg gtagaacagt
     2941 cgcaattggg atcccgtcgc aatggcctcg ttcaactccg cggtgaagac cccgacgtca
     3001 tccgggtgga tcaggtctct gaggaacaag tcaatgattt cgggcggctt atagccggtc
     3061 aggggttcta cgctgggcga aacatgcttg atccgtccgt tggcatccag aatgtgttcc
     3121 cagtcctgga gctcttcgac aaccttggcg ggccaattgc ggcgcttggt gaattcggtc
     3181 agggtgcttc cgccgcccat ttcggaccgg gatccgcgcc cgatggtggg acggtatgaa
     3241 taatccggaa tatttatagg tttttttatt acaaaactgt tacgaaaaca gtaaaatact
     3301 tatttatttg cgagatggtt atcattttaa ttatctccat gatctattaa tattccggag
     3361 tatacgtagc caaccactag aactatagct agagtcctgg gcgaacaaac gatgctcgcc
     3421 ttccagaaaa ccgaggatgc gaaccacttc atccggggtc agcaccaccg gcaagcgccg
     3481 cgacggccga ggtcttccga tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc
     3541 gtagaagaac agcaaggccg ccaatgcctg acgatgcgtg gagaccgaaa ccttgcgctc
     3601 gttcgccagc caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg
     3661 cacaccgtgg aaacggatga aggcacgaac ccagttgaca taagcctgtt cggttcgtaa
     3721 actgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     3781 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc
     3841 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3901 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag
     3961 tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc
     4021 tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac atcagccgga
     4081 ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga
     4141 ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg agcagccgcg
     4201 tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc agggcattgc
     4261 caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta
     4321 cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg
     4381 ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat tcgttcaagc
     4441 cgagatcggc ttcccggccg cggagttgtt cggtaaattg tcacaacgcc gcgaatatag
     4501 tctttaccat gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa
     4561 aatgaagagt ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg
     4621 actgatttca gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc
     4681 tattttgttc agtttaagac tttattgtcc gcccacaccc gcttacgcag ggcatccatt
     4741 tattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtctatgcg gtgtgaaata
     4801 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     4861 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4921 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4981 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5041 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5101 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5161 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5221 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5281 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5341 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5401 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5461 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5521 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5581 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5641 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5701 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5761 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5821 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5881 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5941 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6001 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6061 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6121 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6181 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6241 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6301 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6361 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6421 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6481 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6541 gcatctttta ctttcacctc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6601 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6661 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6721 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcac
//
