LOCUS       plasmid                 6557 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     mobile_element  3270..3494
                     /note="pLannotate"
                     /label="Tn7R"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     mobile_element  6187..6352
                     /note="pLannotate"
                     /label="Tn7L"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     polyA_signal    complement(6381..6515)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(5419..5523)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(2029..2120)
                     /note="pLannotate"
                     /label="polyhedrin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(4558..5418)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(124..813)
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="96.6"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(3799..4387)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2670..3203
                     /note="pLannotate"
                     /label="GmR"
                     /database="snapgene"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(5549..6003)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(835..1974)
                     /note="pLannotate"
                     /label="wc-2 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="71.7"
                     /fragment="True"
                     /other="CDS"
     promoter        2472..2500
                     /note="pLannotate"
                     /label="Pc promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3562..3613
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(815..834)
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.2"
                     /fragment="True"
                     /other="CDS"
     CDS             2147..2494
                     /note="pLannotate"
                     /label="YPR2_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="94.8"
                     /match_length="88.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(join(6538..6557,1..64))
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6008..6024)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
       61 gatcctctag tacttctcga caagcttggt accgcatgcc tcgagttagt ggtgatggtg
      121 gtggtgggtg atgccagcgg ctgtcacgaa ttccagcagg accatgtggt ctctcttctc
      181 gttagggtcc ttagacagag cggactgtgt gctcaggtag tggttgtcag gcagcagcac
      241 ggggccgtcg ccgatagggg tgttctgctg gtagtgatcg gccagctgca cgctgccatc
      301 ttccacattg tgtctgatct tgaagttggc cttgatacca ttcttctgct tgtcggctgt
      361 gatgtacacg ttgtggctgt tgaagttgta ttccagcttg tggcccagga tgttgccatc
      421 ttccttgaag tcgatgccct tcagctcgat tctgttcacc agtgtatctc cctcgaactt
      481 cacctcggct ctggtcttat aggtgccatc gtctttaaaa gagatggtcc gctcctgcac
      541 gtagccctca ggcatggcgg atttgaagaa atcatgccgc ttcatgtggt cggggtatct
      601 gctaaaacat tgcacgccgt atgtcagggt tgtgaccagg gtgggccaag gcacaggcag
      661 tttgccggtg gtgcagataa atttcagggt cagctttccg ttggtggcgt cgccttcgcc
      721 ctcgccccgc acgctgaact tgtgtccatt cacgtcgccg tcgagctcaa ccagaattgg
      781 caccactccg gtgaacagtt cctcgccctt gcttccctga aaatacaggt tttctcccat
      841 atgatcgccc atgggtgtat ggatgtcgtt gtgaccgcct atacctccgc cgttattgtt
      901 gttgttggcg ttcttctttt tctctttctt tgcccagcga agaccgcagg cattgcatag
      961 tgtcttgggt ccactagggc cttttcgcca ttcgggggaa tcgagcgtac cgcagtcggt
     1021 gcacacgtat tcctctgcaa ccttgatctt cttcttcttc tctcccgtcc ggggatctcg
     1081 gtctagggga atcgcgattc ccgcatcacc cttaatgagc gtcgggctgg cattacccgt
     1141 tgtgatacca tgacttcttt caccctcctg gtacttgagt cctgtaagca tttcaatcgt
     1201 gtctgcgtga ttgccctcgt aacgcaacat cttttcgcgt atagaatccg gccggcttcc
     1261 ggcaatgcct tccaggttct cccgcgtcag cgcaatgtta agcgacgacg ccggctggct
     1321 gggtggtggc atcataacgt cagcttgggc attcatggga atgtagaagg gcgtcattcc
     1381 catctgggtc gcggtatcgt cggacggcgt aacatcagat cgtccctcct ggctcattcg
     1441 ccatgtccta tgcgactctt cctgctcctc ctgctcctct ctgcgcagct cggcaatcct
     1501 gcgcttcagt ctttcgtttt caatcttgtg ttccagaaag gaatccaaca gacccgcgtt
     1561 cttggtcggg tagggtcgag ccatcatgaa cacggcctgg cagaaaggcg actggttctg
     1621 tggattgggt gcaaacttgg cggcggcgat gtgcgcatga ccaacagtct cgaaaatggt
     1681 ccagtttcca tcctttttcc ggaaccggta gaacagtcgc aattgggatc ccgtcgcaat
     1741 ggcctcgttc aactccgcgg tgaagacccc gacgtcatcc gggtggatca ggtctctgag
     1801 gaacaagtca atgatttcgg gcggcttata gccggtcagg ggttctacgc tgggcgaaac
     1861 atgcttgatc cgtccgttgg catccagaat gtgttcccag tcctggagct cttcgacaac
     1921 cttggcgggc caattgcggc gcttggtgaa ttcggtcagg gtgcttccgc cgcccatttc
     1981 ggaccgggat ccgcgcccga tggtgggacg gtatgaataa tccggaatat ttataggttt
     2041 ttttattaca aaactgttac gaaaacagta aaatacttat ttatttgcga gatggttatc
     2101 attttaatta tctccatgat ctattaatat tccggagtat acgtagccaa ccactagaac
     2161 tatagctaga gtcctgggcg aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa
     2221 ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt cttccgatct
     2281 cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc aaggccgcca
     2341 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg
     2401 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     2461 cacgaaccca gttgacataa gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc
     2521 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     2581 ttttcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc
     2641 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2701 agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt cgcacatgta
     2761 ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc ggtcgtgagt
     2821 tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc gggaacttgc
     2881 tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt gttggcgctc
     2941 tcgcggctta cgttctgccc aagtttgagc agccgcgtag tgagatctat atctatgatc
     3001 tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc aatctcctca
     3061 agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat tacggtgacg
     3121 atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg cactttgata
     3181 tcgacccaag taccgccacc taacaattcg ttcaagccga gatcggcttc ccggccgcgg
     3241 agttgttcgg taaattgtca caacgccgcg aatatagtct ttaccatgcc cttggccacg
     3301 cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg ctttagccat
     3361 aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt tacaactatt
     3421 ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt ttaagacttt
     3481 attgtccgcc cacacccgct tacgcagggc atccatttat tactcaaccg taaccgattt
     3541 tgccaggtta cgcggctggt ctatgcggtg tgaaataccg cacagatgcg taaggagaaa
     3601 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3661 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3721 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3781 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3841 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3901 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3961 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4021 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4081 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4141 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4201 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4261 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4321 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4381 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4441 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4501 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4561 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4621 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4681 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4741 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4801 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4861 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4921 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4981 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5041 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5101 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5161 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5221 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5281 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5341 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5401 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5461 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5521 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     5581 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5641 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5701 agtccactat taagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5761 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5821 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5881 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     5941 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6001 cgtcccattc gccattcagg ctgcaaataa gcgttgatat tcagtcaatt acaaacatta
     6061 ataacgaaga gatgacagaa aaattttcat tctgtgacag agaaaaagta gccgaagatg
     6121 acggtttgtc acatggagtt ggcaggatgt ttgattaaaa acataacagg aagaaaaatg
     6181 ccccgctgtg ggcggacaaa atagttggga actgggaggg gtggaaatgg agtttttaag
     6241 gattatttag ggaagagtga caaaatagat gggaactggg tgtagcgtcg taagctaata
     6301 cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg ttcggatctc
     6361 ctaggctcaa gcagtgatca gatccagaca tgataagata cattgatgag tttggacaaa
     6421 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6481 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6541 tgtttcaggt tcagggg
//
