LOCUS       plasmid                 6557 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     mobile_element  4411..4635
                     /note="pLannotate"
                     /label="Tn7R"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     mobile_element  771..936
                     /note="pLannotate"
                     /label="Tn7L"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="mobile_element"
     polyA_signal    complement(965..1099)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3..107)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(3170..3261)
                     /note="pLannotate"
                     /label="polyhedrin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(5699..6557,1..2))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1265..1954)
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="96.6"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4940..5528)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3811..4344
                     /note="pLannotate"
                     /label="GmR"
                     /database="snapgene"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(133..587)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(1976..3115)
                     /note="pLannotate"
                     /label="wc-2 (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="71.7"
                     /fragment="True"
                     /other="CDS"
     promoter        3613..3641
                     /note="pLannotate"
                     /label="Pc promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    4703..4754
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1956..1975)
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.2"
                     /fragment="True"
                     /other="CDS"
     CDS             3288..3635
                     /note="pLannotate"
                     /label="YPR2_ECOLX (fragment)"
                     /database="swissprot"
                     /identity="94.8"
                     /match_length="88.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1122..1205)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(592..608)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.8"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
       61 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      121 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
      181 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
      241 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaagaa
      301 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      361 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      421 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
      481 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      541 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
      601 caggctgcaa ataagcgttg atattcagtc aattacaaac attaataacg aagagatgac
      661 agaaaaattt tcattctgtg acagagaaaa agtagccgaa gatgacggtt tgtcacatgg
      721 agttggcagg atgtttgatt aaaaacataa caggaagaaa aatgccccgc tgtgggcgga
      781 caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat ttagggaaga
      841 gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa ttaaaaatga
      901 caaaatagtt tggaactaga tttcacttat ctggttcgga tctcctaggc tcaagcagtg
      961 atcagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     1021 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     1081 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     1141 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     1201 tgatcctcta gtacttctcg acaagcttgg taccgcatgc ctcgagttag tggtgatggt
     1261 ggtggtgggt gatgccagcg gctgtcacga attccagcag gaccatgtgg tctctcttct
     1321 cgttagggtc cttagacaga gcggactgtg tgctcaggta gtggttgtca ggcagcagca
     1381 cggggccgtc gccgataggg gtgttctgct ggtagtgatc ggccagctgc acgctgccat
     1441 cttccacatt gtgtctgatc ttgaagttgg ccttgatacc attcttctgc ttgtcggctg
     1501 tgatgtacac gttgtggctg ttgaagttgt attccagctt gtggcccagg atgttgccat
     1561 cttccttgaa gtcgatgccc ttcagctcga ttctgttcac cagtgtatct ccctcgaact
     1621 tcacctcggc tctggtctta taggtgccat cgtctttaaa agagatggtc cgctcctgca
     1681 cgtagccctc aggcatggcg gatttgaaga aatcatgccg cttcatgtgg tcggggtatc
     1741 tgctaaaaca ttgcacgccg tatgtcaggg ttgtgaccag ggtgggccaa ggcacaggca
     1801 gtttgccggt ggtgcagata aatttcaggg tcagctttcc gttggtggcg tcgccttcgc
     1861 cctcgccccg cacgctgaac ttgtgtccat tcacgtcgcc gtcgagctca accagaattg
     1921 gcaccactcc ggtgaacagt tcctcgccct tgcttccctg aaaatacagg ttttctccca
     1981 tatgatcgcc catgggtgta tggatgtcgt tgtgaccgcc tatacctccg ccgttattgt
     2041 tgttgttggc gttcttcttt ttctctttct ttgcccagcg aagaccgcag gcattgcata
     2101 gtgtcttggg tccactaggg ccttttcgcc attcggggga atcgagcgta ccgcagtcgg
     2161 tgcacacgta ttcctctgca accttgatct tcttcttctt ctctcccgtc cggggatctc
     2221 ggtctagggg aatcgcgatt cccgcatcac ccttaatgag cgtcgggctg gcattacccg
     2281 ttgtgatacc atgacttctt tcaccctcct ggtacttgag tcctgtaagc atttcaatcg
     2341 tgtctgcgtg attgccctcg taacgcaaca tcttttcgcg tatagaatcc ggccggcttc
     2401 cggcaatgcc ttccaggttc tcccgcgtca gcgcaatgtt aagcgacgac gccggctggc
     2461 tgggtggtgg catcataacg tcagcttggg cattcatggg aatgtagaag ggcgtcattc
     2521 ccatctgggt cgcggtatcg tcggacggcg taacatcaga tcgtccctcc tggctcattc
     2581 gccatgtcct atgcgactct tcctgctcct cctgctcctc tctgcgcagc tcggcaatcc
     2641 tgcgcttcag tctttcgttt tcaatcttgt gttccagaaa ggaatccaac agacccgcgt
     2701 tcttggtcgg gtagggtcga gccatcatga acacggcctg gcagaaaggc gactggttct
     2761 gtggattggg tgcaaacttg gcggcggcga tgtgcgcatg accaacagtc tcgaaaatgg
     2821 tccagtttcc atcctttttc cggaaccggt agaacagtcg caattgggat cccgtcgcaa
     2881 tggcctcgtt caactccgcg gtgaagaccc cgacgtcatc cgggtggatc aggtctctga
     2941 ggaacaagtc aatgatttcg ggcggcttat agccggtcag gggttctacg ctgggcgaaa
     3001 catgcttgat ccgtccgttg gcatccagaa tgtgttccca gtcctggagc tcttcgacaa
     3061 ccttggcggg ccaattgcgg cgcttggtga attcggtcag ggtgcttccg ccgcccattt
     3121 cggaccggga tccgcgcccg atggtgggac ggtatgaata atccggaata tttataggtt
     3181 tttttattac aaaactgtta cgaaaacagt aaaatactta tttatttgcg agatggttat
     3241 cattttaatt atctccatga tctattaata ttccggagta tacgtagcca accactagaa
     3301 ctatagctag agtcctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     3361 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     3421 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     3481 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     3541 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     3601 gcacgaaccc agttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
     3661 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     3721 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
     3781 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     3841 cagggcagtc gccctaaaac aaagttaggt ggctcaagta tgggcatcat tcgcacatgt
     3901 aggctcggcc ctgaccaagt caaatccatg cgggctgctc ttgatctttt cggtcgtgag
     3961 ttcggagacg tagccaccta ctcccaacat cagccggact ccgattacct cgggaacttg
     4021 ctccgtagta agacattcat cgcgcttgct gccttcgacc aagaagcggt tgttggcgct
     4081 ctcgcggctt acgttctgcc caagtttgag cagccgcgta gtgagatcta tatctatgat
     4141 ctcgcagtct ccggcgagca ccggaggcag ggcattgcca ccgcgctcat caatctcctc
     4201 aagcatgagg ccaacgcgct tggtgcttat gtgatctacg tgcaagcaga ttacggtgac
     4261 gatcccgcag tggctctcta tacaaagttg ggcatacggg aagaagtgat gcactttgat
     4321 atcgacccaa gtaccgccac ctaacaattc gttcaagccg agatcggctt cccggccgcg
     4381 gagttgttcg gtaaattgtc acaacgccgc gaatatagtc tttaccatgc ccttggccac
     4441 gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca
     4501 taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat
     4561 tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt
     4621 tattgtccgc ccacacccgc ttacgcaggg catccattta ttactcaacc gtaaccgatt
     4681 ttgccaggtt acgcggctgg tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     4741 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5341 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6301 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6541 ggaaatgttg aatactc
//
