LOCUS       plasmid                 6407 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             1221..2081
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2252..2840
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3267..3458)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             5207..6307
                     /note="pLannotate"
                     /label="MBP"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4097..4997)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="75.7"
                     /fragment="True"
                     /other="CDS"
     terminator      562..609
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             5168..5197
                     /note="pLannotate"
                     /label="10xHis"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(975..1003)
                     /note="pLannotate"
                     /label="tet promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     RBS             5126..5148
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        5019..5037
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3026..3165)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        1116..1220
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             108..389
                     /note="pLannotate"
                     /label="hh2b (fragment)"
                     /database="swissprot"
                     /identity="90.4"
                     /match_length="68.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(635..927)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3475..3855
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     CDS             505..554
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             6362..6378
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cgcatagttc tgcatgatgg gttaggtttt cgcatttatc gtgaaacgct ttcgcgtttt
       61 tcgtgcgccg cttcattctg catgatgggt taggttttcg catttatcgt gaaacgcttt
      121 cgcgtttttc gtgcgccgct tctctgaagc aggttcaccc ggataccggc atcagcaatc
      181 gtgcaatgag catcctcaac agcttcgtga acgacatctt tgaacgcgtg gccacggagg
      241 catctaagct ggcggcatac aacaagaaaa gcaccattag cagccgtgag atccaaacca
      301 gcgtgcgtct gattttgcca ggtgaactgg cgaaacatgc cgtttccgag ggtactaaag
      361 cggtcaccaa atactcctct tccactaaga cggagacttc gcaagtagcg ccggcgtaat
      421 aataacattg gaagtggata acggatccgc gatcgcggcg cgccacctgg tggccggccg
      481 gtaccacgcg tgcgcgctga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      541 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      601 ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat
      661 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
      721 gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc
      781 tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc
      841 cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat
      901 gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg
      961 tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa
     1021 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     1081 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1141 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1201 aacattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1261 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1321 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1381 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1441 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     1501 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1561 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1621 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1681 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1741 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     1801 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1861 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1921 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1981 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2041 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2101 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2161 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2221 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2281 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2341 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2401 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2461 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2521 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2581 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2641 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2701 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2761 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2821 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2881 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2941 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3001 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     3061 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3121 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     3181 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3241 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3301 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     3361 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     3421 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     3481 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     3541 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     3601 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     3661 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     3721 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     3781 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     3841 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     3901 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg
     3961 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc
     4021 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg
     4081 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg
     4141 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg
     4201 ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat gatcgaagtt aggctggtaa
     4261 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca
     4321 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg
     4381 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc
     4441 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     4501 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     4561 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     4621 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     4681 aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact
     4741 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     4801 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     4861 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     4921 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     4981 gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggag
     5041 accacaacgg tttccctcta gtgccggctc cggagagctc tttaattaag cggccgccct
     5101 gcaggactcg agttctagaa ataattttgt ttaactttaa gaaggagata tacatatgaa
     5161 atcttctcac catcaccatc accatcacca tcaccatggt tcttctatga aaatcgaaga
     5221 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
     5281 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
     5341 agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca
     5401 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
     5461 gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat
     5521 tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa
     5581 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
     5641 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
     5701 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
     5761 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
     5821 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
     5881 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
     5941 aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc
     6001 aggtattaac gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct
     6061 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
     6121 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacta tggaaaacgc
     6181 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg
     6241 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc
     6301 gcagactaat tcgagctcga acaacaacaa caataacaat aacaacaacc tcgggatcga
     6361 ggaaaacctg tacttccaat ccaatgcaat gcctccaaaa cccgctg
//
