LOCUS       plasmid                 5031 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             3933..4793
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      join(4964..5031,1..521)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(948..1139)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1778..2678)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="75.7"
                     /fragment="True"
                     /other="CDS"
     terminator      3274..3321
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             2849..2878
                     /note="pLannotate"
                     /label="10xHis"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3687..3715)
                     /note="pLannotate"
                     /label="tet promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     RBS             2807..2829
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        2700..2718
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(707..846)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             2888..3258
                     /note="pLannotate"
                     /label="MBP (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="33.7"
                     /fragment="True"
                     /other="CDS"
     promoter        3828..3932
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3347..3639)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.6"
                     /fragment="True"
                     /other="CDS"
     CDS             1156..1536
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
ORIGIN
        1 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
       61 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      601 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      661 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
      721 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
      781 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
      841 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
      901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
      961 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     1021 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     1081 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     1141 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     1201 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     1261 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     1321 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
     1381 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
     1441 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
     1501 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
     1561 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg
     1621 ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
     1681 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
     1741 gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
     1801 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg
     1861 ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa
     1921 tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt
     1981 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     2041 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     2101 ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     2161 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     2221 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     2281 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     2341 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     2401 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     2461 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     2521 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     2581 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     2641 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt
     2701 aatacgactc actataggga gaccacaacg gtttccctct agtgccggct ccggagagct
     2761 ctttaattaa gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta
     2821 agaaggagat atacatatga aatcttctca ccatcaccat caccatcacc atcaccatgg
     2881 ttcttctatg aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta
     2941 taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt
     3001 tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc
     3061 tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc
     3121 tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt
     3181 acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta
     3241 taacaaagat ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     3301 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg
     3361 tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg
     3421 gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa
     3481 cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc
     3541 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
     3601 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     3661 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg
     3721 agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3781 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3901 aaatgcttca ataacattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4021 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4081 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4141 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     4201 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4261 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4321 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4381 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4441 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     4501 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4561 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg g
//
