LOCUS plasmid 6395 bp DNA circular SYN 22-JAN-2026 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE . ORGANISM . . COMMENT Annotated with pLannotate v1.2.0 FEATURES Location/Qualifiers CDS complement(4379..5239) /note="pLannotate" /label="AmpR" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" rep_origin complement(3620..4208) /note="pLannotate" /label="ori" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="rep_origin" CDS 3002..3193 /note="pLannotate" /label="rop" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" CDS complement(153..1253) /note="pLannotate" /label="MBP" /database="snapgene" /identity="99.8" /match_length="100.0" /fragment="False" /other="CDS" CDS 1463..2363 /note="pLannotate" /label="TcR (fragment)" /database="snapgene" /identity="100.0" /match_length="75.7" /fragment="True" /other="CDS" terminator complement(5851..5898) /note="pLannotate" /label="T7 terminator" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="terminator" CDS complement(1263..1292) /note="pLannotate" /label="10xHis" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" promoter 5457..5485 /note="pLannotate" /label="tet promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" RBS complement(1312..1334) /note="pLannotate" /label="RBS" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="RBS" promoter complement(1423..1441) /note="pLannotate" /label="T7 promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS complement(join(6071..6395,1..71)) /note="pLannotate" /label="hh2b" /database="swissprot" /identity="89.1" /match_length="96.4" /fragment="False" /other="CDS" misc_feature 3295..3434 /note="pLannotate" /label="bom" /database="snapgene" /identity="99.3" /match_length="100.0" /fragment="False" /other="misc_feature" promoter complement(5240..5344) /note="pLannotate" /label="AmpR promoter" /database="snapgene" /identity="99.0" /match_length="100.0" /fragment="False" /other="promoter" CDS 5533..5825 /note="pLannotate" /label="TcR (fragment)" /database="snapgene" /identity="100.0" /match_length="24.6" /fragment="True" /other="CDS" CDS complement(2605..2985) /note="pLannotate" /label="YPB1_ECOLX" /database="swissprot" /identity="99.2" /match_length="96.2" /fragment="False" /other="CDS" CDS complement(5906..5955) /note="pLannotate" /label="SET1 (fragment)" /database="snapgene" /identity="100.0" /match_length="39.7" /fragment="True" /other="CDS" CDS complement(82..98) /note="pLannotate" /label="TEV site (fragment)" /database="snapgene" /identity="100.0" /match_length="81.0" /fragment="True" /other="CDS" ORIGIN 1 ttctccggag cttttgacgc cgtcgccgga gccttcgaag cgggcttttt atcagcgggt 61 tttggaggca ttgcattgga ttggaagtac aggttttcct cgatcccgag gttgttgtta 121 ttgttattgt tgttgttgtt cgagctcgaa ttagtctgcg cgtctttcag ggcttcatcg 181 acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata ccagaaagcg 241 gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat agtggcggca 301 atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgctacggc acccagcggt 361 ttgtctttat taaccgcttc cagaccttca tcagtcagca gatagttttc gaggaactct 421 tttgccagct ctttgttcgg actggcggcg ttaatacctg cgctcagcac gccaacgaac 481 ggtttggatg gttgaccctt gaaggtcggc agtaccgtta caccataatt cactttgctg 541 gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc gcctttatta 601 aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt tttaatcagg 661 tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacgcccac gtctttaatg 721 tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat cagcggccag 781 gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc tttcagttct 841 ttatccagcg ccgggatctc ttcccaggtt tttggcgggt tcggcagcag atctttgtta 901 taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc gttgtaacgt 961 acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtccgg ggtgatttca 1021 gccaacaggc cagattgagc gtagccacca aagcggtcgt gtgcccagaa gataatgtca 1081 gggccatcgc cagttgccgc aacctgtggg aatttctctt ccagtttatc cggatgctca 1141 acggtgactt taattccggt atctttctcg aatttcttac cgacttcagc gagaccgtta 1201 tagcctttat cgccgttaat ccagattacc agtttacctt cttcgatttt catagaagaa 1261 ccatggtgat ggtgatggtg atggtgatgg tgagaagatt tcatatgtat atctccttct 1321 taaagttaaa caaaattatt tctagaactc gagtcctgca gggcggccgc ttaattaaag 1381 agctctccgg agccggcact agagggaaac cgttgtggtc tccctatagt gagtcgtatt 1441 aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg 1501 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 1561 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 1621 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 1681 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 1741 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga 1801 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 1861 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc 1921 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg 1981 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg 2041 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc 2101 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg 2161 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca 2221 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg 2281 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat 2341 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac 2401 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg 2461 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag 2521 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg 2581 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag 2641 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg 2701 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg 2761 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg 2821 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc 2881 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct 2941 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc 3001 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt 3061 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc 3121 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg 3181 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 3241 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc 3301 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag 3361 cagattgtac tgagagtgca ccattgcggt gtgaaatacc gcacagatgc gtaaggagaa 3421 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 3481 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 3661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 3841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 4021 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 4081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 4141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 4201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 4261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 4321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 4381 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 4441 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 4501 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 4561 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 4621 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 4681 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 4741 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 4801 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 4861 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 4921 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 4981 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 5041 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 5101 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 5161 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 5221 ggaaatgttg aatactcata ctcttccttt ttcaatgtta ttgaagcatt tatcagggtt 5281 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 5341 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 5401 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc tcatgtttga 5461 cagcttatca tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt 5521 caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg 5581 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc 5641 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa 5701 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg 5761 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg 5821 tggatatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc 5881 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg 5941 ggctttgtta gcagccggat cagcgcgcac gcgtggtacc ggccggccac caggtggcgc 6001 gccgcgatcg cggatccgtt atccacttcc aatgttatta ttacgccggc gctacttgcg 6061 aagtctccgt cttagtggaa gaggagtatt tggtgaccgc tttagtaccc tcggaaacgg 6121 catgtttcgc cagttcacct ggcaaaatca gacgcacgct ggtttggatc tcacggctgc 6181 taatggtgct tttcttgttg tatgccgcca gcttagatgc ctccgtggcc acgcgttcaa 6241 agatgtcgtt cacgaagctg ttgaggatgc tcattgcacg attgctgatg ccggtatccg 6301 ggtgaacctg cttcagaact ttataaatat agctagagta ggtttcttta cgcgccttgg 6361 tgcgcttctt tttatcaccg ctcgccgcgg tcttt //