LOCUS       plasmid                 6395 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(4379..5239)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(3620..4208)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3002..3193
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(153..1253)
                     /note="pLannotate"
                     /label="MBP"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1463..2363
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="75.7"
                     /fragment="True"
                     /other="CDS"
     terminator      complement(5851..5898)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(1263..1292)
                     /note="pLannotate"
                     /label="10xHis"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        5457..5485
                     /note="pLannotate"
                     /label="tet promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     RBS             complement(1312..1334)
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        complement(1423..1441)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(6071..6395,1..71))
                     /note="pLannotate"
                     /label="hh2b"
                     /database="swissprot"
                     /identity="89.1"
                     /match_length="96.4"
                     /fragment="False"
                     /other="CDS"
     misc_feature    3295..3434
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        complement(5240..5344)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             5533..5825
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2605..2985)
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5906..5955)
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(82..98)
                     /note="pLannotate"
                     /label="TEV site (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttctccggag cttttgacgc cgtcgccgga gccttcgaag cgggcttttt atcagcgggt
       61 tttggaggca ttgcattgga ttggaagtac aggttttcct cgatcccgag gttgttgtta
      121 ttgttattgt tgttgttgtt cgagctcgaa ttagtctgcg cgtctttcag ggcttcatcg
      181 acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata ccagaaagcg
      241 gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat agtggcggca
      301 atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgctacggc acccagcggt
      361 ttgtctttat taaccgcttc cagaccttca tcagtcagca gatagttttc gaggaactct
      421 tttgccagct ctttgttcgg actggcggcg ttaatacctg cgctcagcac gccaacgaac
      481 ggtttggatg gttgaccctt gaaggtcggc agtaccgtta caccataatt cactttgctg
      541 gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc gcctttatta
      601 aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt tttaatcagg
      661 tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacgcccac gtctttaatg
      721 tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat cagcggccag
      781 gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc tttcagttct
      841 ttatccagcg ccgggatctc ttcccaggtt tttggcgggt tcggcagcag atctttgtta
      901 taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc gttgtaacgt
      961 acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtccgg ggtgatttca
     1021 gccaacaggc cagattgagc gtagccacca aagcggtcgt gtgcccagaa gataatgtca
     1081 gggccatcgc cagttgccgc aacctgtggg aatttctctt ccagtttatc cggatgctca
     1141 acggtgactt taattccggt atctttctcg aatttcttac cgacttcagc gagaccgtta
     1201 tagcctttat cgccgttaat ccagattacc agtttacctt cttcgatttt catagaagaa
     1261 ccatggtgat ggtgatggtg atggtgatgg tgagaagatt tcatatgtat atctccttct
     1321 taaagttaaa caaaattatt tctagaactc gagtcctgca gggcggccgc ttaattaaag
     1381 agctctccgg agccggcact agagggaaac cgttgtggtc tccctatagt gagtcgtatt
     1441 aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg
     1501 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     1561 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     1621 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     1681 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     1741 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     1801 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     1861 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     1921 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     1981 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     2041 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     2101 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     2161 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
     2221 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
     2281 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
     2341 ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
     2401 tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
     2461 gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
     2521 cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg
     2581 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
     2641 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
     2701 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
     2761 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
     2821 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
     2881 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
     2941 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
     3001 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
     3061 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
     3121 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
     3181 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     3241 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     3301 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     3361 cagattgtac tgagagtgca ccattgcggt gtgaaatacc gcacagatgc gtaaggagaa
     3421 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3481 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4021 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4381 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4441 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4501 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4561 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4621 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4681 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4741 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4801 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4861 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4921 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4981 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5041 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5101 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5161 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5221 ggaaatgttg aatactcata ctcttccttt ttcaatgtta ttgaagcatt tatcagggtt
     5281 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5341 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5401 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattc tcatgtttga
     5461 cagcttatca tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt
     5521 caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg
     5581 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
     5641 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
     5701 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
     5761 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
     5821 tggatatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc
     5881 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg
     5941 ggctttgtta gcagccggat cagcgcgcac gcgtggtacc ggccggccac caggtggcgc
     6001 gccgcgatcg cggatccgtt atccacttcc aatgttatta ttacgccggc gctacttgcg
     6061 aagtctccgt cttagtggaa gaggagtatt tggtgaccgc tttagtaccc tcggaaacgg
     6121 catgtttcgc cagttcacct ggcaaaatca gacgcacgct ggtttggatc tcacggctgc
     6181 taatggtgct tttcttgttg tatgccgcca gcttagatgc ctccgtggcc acgcgttcaa
     6241 agatgtcgtt cacgaagctg ttgaggatgc tcattgcacg attgctgatg ccggtatccg
     6301 ggtgaacctg cttcagaact ttataaatat agctagagta ggtttcttta cgcgccttgg
     6361 tgcgcttctt tttatcaccg ctcgccgcgg tcttt
//
