LOCUS       plasmid                 6355 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1863..2888)
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(3280..3708)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3754..3978)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4928..5127)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1612..1733)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5792..5896
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(5897..6355,1..402)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4041..4334)
                     /note="pLannotate"
                     /label="Cxcl10"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      573..1161
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(5129..5508)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(2937..3266)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1485..1515
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1449..1470
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(4357..4375)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1523..1539
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     intron          complement(4460..4549)
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="39.1"
                     /fragment="True"
                     /other="intron"
     CDS             complement(1728..1853)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    5681..5733
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4395..4441)
                     /note="pLannotate"
                     /label="IgH signal sequence (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="33.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(4822..4860)
                     /note="pLannotate"
                     /label="Ad SA (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             4580..4789
                     /note="pLannotate"
                     /label="PTP (fragment)"
                     /database="swissprot"
                     /identity="98.6"
                     /match_length="10.4"
                     /fragment="True"
                     /other="CDS"
     CDS             1344..1436
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     intron          complement(4561..4583)
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="10.0"
                     /fragment="True"
                     /other="intron"
     promoter        complement(3729..3750)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
       61 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      121 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      181 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      241 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      301 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      361 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      421 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      481 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1261 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1321 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1381 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     1441 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     1501 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     1561 gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca
     1621 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1681 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg
     1741 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     1801 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg
     1861 tgctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     1921 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     1981 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     2041 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     2101 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     2161 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     2221 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     2281 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     2341 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     2401 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     2461 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     2521 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     2581 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     2641 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     2701 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     2761 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     2821 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     2881 tttttcatca cgtgctgatc agatccgaaa atggatatac aagctcccgg gagctttttg
     2941 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag
     3001 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     3061 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     3121 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     3181 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     3241 gactttccac accctaactg acacacattc cacagaatta attcgcgtta aatttttgtt
     3301 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     3361 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     3421 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     3481 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     3541 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3601 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3661 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc
     3721 tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
     3781 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     3841 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     3901 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     3961 ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggcccg aattcggcgg
     4021 ccgctctaga ctcgagttta aggagccctt ttagaccttt tttggctaaa cgctttcatt
     4081 aaattcttga tggtcttaga ttccggattc agacatctct gctcatcatt ctttttcatc
     4141 gtggcaatga tctcaacacg tgggcaggat aggctcgcag ggatgatttc aagcttccct
     4201 atggccctca ttctcactgg cccgtcatcg atatggatgc agttgcagcg gaccgtcctt
     4261 gcgagaggga tcccttgagt cccactcaga cccagcagga tgaggcagaa aatgacggca
     4321 gcacttgggt tcatggtacc aagcttggtg gcggccccta tagtgagtcg tattaaagtt
     4381 taaacttgga cctgggagtg gacacctgtg gagagaaagg caaagtggat gtcattgtca
     4441 ctcaagtgta tggccagatc gggccaggtg aatatcaaat cctcctcgtt tttggaaact
     4501 gacaatctta gcgcagaagt aatgcccgct tttgagaggg agtactcacc ccaacagctg
     4561 gatctcaagc ctgccacacc tcacctcgac catccgccgt ctcaagaccg cctactttaa
     4621 ttacatcatc agcagcacct ccgccagaaa caaccccgac cgccacccgc tgccgcccgc
     4681 cacggtgctc agcctacctt gcgactgtga ctggttagac gcctttctcg agaggttttc
     4741 cgatccggtc gatgcggact cgctcaggtc cctcggtggc ggagtaccgt tcggaggccg
     4801 acgggtttcc gatccaagag tactggaaag accgcgaaga gtttgtcctc aaccgcgagc
     4861 ccaacagctg gccctcgcag acagcgatgc ggaagagagt gaggatctga cggttcacta
     4921 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaacg
     4981 gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     5041 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     5101 cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     5161 actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag gcgggccatt
     5221 taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga tgtactgcca
     5281 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5341 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     5401 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     5461 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa catggcggtc
     5521 atattggaca tgagccaata taaatgtact cgcgaagcag cgcaaaacgc ctaaccctaa
     5581 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     5641 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     5701 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     5761 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5821 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5881 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5941 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6001 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6061 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatg
//
