LOCUS       plasmid                 2875 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     protein_bind    complement(1759..1858)
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(join(2827..2875,1..750))
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="97.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2103..2691)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      888..934
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    1132..1231
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1036..1079)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    866..888
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.3"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
       61 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
      121 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
      181 cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt
      241 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
      301 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
      361 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
      421 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
      481 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
      541 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
      601 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
      661 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
      721 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
      781 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
      841 ttgagacaca acgtggcttt cccccgccgc tctagaacta gtggatccaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcgcat tatacgagac
      961 gtccaggttg ggatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact
     1021 gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc
     1081 atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc tcaaataatg
     1141 attttatttt gactgatagt gacctgttcg ttgcaacaga ttgatgagca atgctttttt
     1201 ataatgccaa ctttgtacaa aaaagcaggc tccgccacca tgatccaaac acggactttt
     1261 ttactctctt ttgttttctt tgtcttcact ttttcggtgg ttgttgctca gacggccaaa
     1321 agtccagtaa caaactgcac tataaaatcc aacacaactt gtgaagattg tttgaagatt
     1381 gtttcgtgtt tgtggtgcat ctcaagccag aaatgcattg actatccagt gaacagcgtt
     1441 ctgccctctc atagtgtttg tccactttca gatgcacgat ggggactgtg ttggatgaat
     1501 ttccagattc tcatcattac tatctctgtg atcgcgggag tgattgtcat cgccatattc
     1561 gtctgctgtt tctgctgctg taaatgtgaa aacattggca ggtctaaaag gagtgaggag
     1621 cgaatggaga atcaagctag tctgagaaag tttcgtcatg aggagaggaa agcagagatg
     1681 aagcagagac atgatgaaat gcgattgaaa tatggtttga agaaggataa ccagtactcc
     1741 agatttgaga acagctagac ccagctttct tgtacaaagt tggcattata agaaagcatt
     1801 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgag
     1861 gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata
     1921 gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc
     1981 gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag
     2041 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2101 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2161 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2221 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2281 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2341 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2401 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2461 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2521 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2581 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2641 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2701 ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag
     2761 cgtaaatggg tagggggctt caaatcgtcc gctctgccag tgttacaacc aattaacaaa
     2821 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatca
//
