LOCUS       plasmid                 2551 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     protein_bind    complement(1795..1894)
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(86..803)
                     /note="pLannotate"
                     /label="KanR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="88.6"
                     /fragment="True"
                     /other="CDS"
     terminator      939..985
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     rep_origin      complement(join(2139..2551,1..4))
                     /note="pLannotate"
                     /label="ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="70.8"
                     /fragment="True"
                     /other="rep_origin"
     promoter        1113..1131
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    1168..1267
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(1973..1988)
                     /note="pLannotate"
                     /label="lac operator (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="64.0"
                     /fragment="True"
                     /other="protein_bind"
     misc_feature    917..939
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.3"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 ggtgtttaac caacgagtct cttgggaccc atagagtttt cgcatttatc gtgaaacgct
       61 ttcgcgtttt tcgtgcgccg cttcaaagga gaaaactcac cgaggcagtt ccataggatg
      121 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
      181 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
      241 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
      301 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
      361 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
      421 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
      481 aatacctgga atgctgtttt tccggggatc gcagtggtga gtaaccatgc atcatcagga
      541 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
      601 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
      661 ggcgcatcgg gcttcccata caagcgatag attgtcgcac ctgattgccc gacattatcg
      721 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgac
      781 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
      841 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
      901 acacgggctt tcccccgccg ctctagaact agtggatcca aataaaacga aaggctcagt
      961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcgca ttatacgaga cgtccaggtt
     1021 gggatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac
     1081 cacagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag gggatatcgc
     1141 ggccgcgtta acgctaccat ggagctccaa ataatgattt tattttgact gatagtgacc
     1201 tgttcgttgc aacaaattga taagcaatgc ttttttataa tgccaacttt gtataataaa
     1261 gcaggctccg ccaccatgat ccaaacacgg acttttttac tctcttttgt tttctttgtc
     1321 ttcacttttt cggtggttgt tgctcagacg gccaaaagtc cagtaacaaa ctgcactata
     1381 aaatccaaca caacttgtga agattgtttg aagattgttt cgtgtttgtg gtgcatctca
     1441 agccagaaat gcattgacta tccagtgaac agcgttctgc cctctcatag tgtttgtcca
     1501 ctttcagatg cacgatgggg actgtgttgg atgaatttcc agattctcat cattactatc
     1561 tctgtgatcg cgggagtgat tgtcatcgcc atattcgtct gctgtttctg ctgctgtaaa
     1621 tgtgaaaaca ttggcaggtc taaaaggagt gaggagcgaa tggagaatca agctagtctg
     1681 gaaaagtttc gtcatgagga gaggaaagca gagatgaagc agagacatga tgaaatgcga
     1741 ttggaatatg gtttgaagaa ggataaccag tactccagat ttgagaacag ctagacccag
     1801 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1861 aggtcactat cagtcaaaat aaaatcatta tttgaggcta ggtggaggct cagtgatgat
     1921 aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta
     1981 tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg
     2041 agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa
     2101 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2521 tatgtaggcg gtgctacaga gttcttgaag t
//
