LOCUS       plasmid                 2691 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(join(2080..2691,1..198))
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(1345..1933)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      complement(813..856)
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      988..1015
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    463..483
                     /note="pLannotate"
                     /label="attB3"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        332..350
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    536..659
                     /note="pLannotate"
                     /label="attR2"
                     /database="snapgene"
                     /identity="98.4"
                     /match_length="99.2"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    387..472
                     /note="pLannotate"
                     /label="attL1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="86.0"
                     /fragment="True"
                     /other="protein_bind"
     CDS             complement(1070..1162)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             730..766
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
       61 atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
      121 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tcgacgtttc
      181 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
      241 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg
      301 ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcgcg
      361 gccgcgttaa cgctaccatg gagctccaaa taatgatttt attttgactg atagtgacct
      421 gttcgttgca acaaattgat aagcaatgct tttttataat gccaactttg tataataaag
      481 ttgctcgaga ctagttacgt acctgcaggg tcgacccgcg ggacgtcggc gcgccccact
      541 ttgtacaaga aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
      601 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatgca gtcactatga
      661 accaactact tagatggtat tagtgacctg tagaattcga gctctagagc tgcagggccc
      721 gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc atgctagcgt
      781 taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcggaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      901 cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg gtggcgggca ggacgcccgc
      961 cataaactgc caggcatcaa actaagcaga aggccatcct gacggatggc ctttttgcgt
     1021 ttctacaaac tcttcctggc tagcggtacg cgtattaatt gcgttgcgct cactgcccgc
     1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1981 cgcgtaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt
     2041 aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact catcgagcat
     2101 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     2161 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     2221 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     2281 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     2341 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     2401 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     2461 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     2521 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     2581 tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     2641 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca t
//
