LOCUS       plasmid                 3645 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2736..3551)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(1668..1767)
                     /note="pLannotate"
                     /label="attL2"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(2012..2600)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      44..90
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             405..1664
                     /note="pLannotate"
                     /label="IRF8"
                     /database="swissprot"
                     /identity="56.0"
                     /match_length="98.6"
                     /fragment="False"
                     /other="CDS"
     protein_bind    288..387
                     /note="pLannotate"
                     /label="attL1"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(192..235)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="25.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    22..44
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.3"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 gacacaacgt ggctttcccc cgccgctcta gaactagtgg atccaaataa aacgaaaggc
       61 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcgcattata cgagacgtcc
      121 aggttgggat acctgaaaca aaacccatcg tacggccaag gaagtctcca ataactgtga
      181 tccaccacaa gcgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc
      241 ataatccgca cgcatctgga ataaggaagt gccattccgc ctgacctcaa ataatgattt
      301 tattttgact gatagtgacc tgttcgttgc aacagattga tgagcaatgc ttttttataa
      361 tgccaacttt gtacaaaaaa gcaggctccg ccaccatgaa ctcgggcggt cgcagactga
      421 aacagtggct tatagaacag ataaacagta acatctataa tggactgcag tgggaggatg
      481 aggaccgcac tatgtttcga atcccctgga aacatgcggg aaaacaagat tacaatcaag
      541 aggtggatgc gtccattttc aaagcgtggg caatatttaa agggaagttt aaggaggggg
      601 ataaagctga accagcgaca tggaagacca gattgcgctg tgcactcaac aaaagcccag
      661 attttgagga agttactgac cgatcccagc ttgacatttc tgagccctac aaagtgtacc
      721 ggattgtgcc agaggaggaa caaaagttag gtaaaggcac agtcaccacc gtaaaagaca
      781 ccactgacat ggactgctca ccagacttgg atgaaattat taaagagtct tcaaatgatg
      841 aatacatggg catcttgaga agtagtcatt ctcccctgga tgaacgcagt agcatgcctt
      901 cagtgcaaga atggtggcag caaggacctc tcaatgctgc tgttgttcat caggatcctg
      961 caggctcatt aaattctgca ttctctcaga tgttgatttc tttctactac ggaggtcaga
     1021 tggtggacaa catggtgacc actcatccag agggctgccg tatctccccc tgcctgccct
     1081 ctacggctaa tggcttcttg tacgggtcag acagcctcca gaacatttac ttcccatcca
     1141 ttgacggcat taagaatgag cgccagcgtc acgtcacccg caaacttttc agtcacttgg
     1201 agcgcggcgt actgctgcgt gccaacagag agggcatttt tatcaagcgt ctgtgccaga
     1261 gcagagtgtt ctggattggc caggatgctc ggtacaaccc ctgcaaacta gagcgagatg
     1321 ctgtggtgaa gatcttcgat acagccagat tcctacaagc actgcagttg tatcaggatg
     1381 gtcattatca ggctccagag cccactgtca cactctgctt cggggaagag tttaacgatt
     1441 tcagcactgt caagagcaaa ttaatcattg tggagatcac agcatggaac tgtcagcagc
     1501 ttttgaatgc agtgactgca cggcgaaccc agtgcagtag tgggaatatg gaaatctcag
     1561 acaacttagt cagtgatcag atggcttgta tatatcagga cctgtgcagc tacccagttc
     1621 ctccacgagc ttcctgtttc agggacaact tacagatacc agtctgaacc cagctttctt
     1681 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1741 tatcagtcaa aataaaatca ttatttgagg ctaggtggag gctcagtgat gataagtctg
     1801 cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1861 agagggcaca atcctattcc gcgctatccg acaatctcca agacattagg tggagttcag
     1921 ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc aaaaggccag caaaaggcca
     1981 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2041 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2101 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2161 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2221 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2281 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2341 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2401 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2461 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2521 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2581 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctctat
     2641 tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc aaatcgtccg
     2701 ctctgccagt gttacaacca attaacaaat tctgattaga aaaactcatc gagcatcaaa
     2761 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2821 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2881 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2941 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     3001 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     3061 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     3121 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     3181 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     3241 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     3301 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     3361 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     3421 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3481 tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga
     3541 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     3601 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttga
//
