LOCUS       plasmid                 2691 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             join(2155..2691,1..273)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      420..1008
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      1497..1540
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(1338..1365)
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    complement(1870..1890)
                     /note="pLannotate"
                     /label="attB3"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(2003..2021)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1694..1817)
                     /note="pLannotate"
                     /label="attR2"
                     /database="snapgene"
                     /identity="98.4"
                     /match_length="99.2"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(1881..1966)
                     /note="pLannotate"
                     /label="attL1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="86.0"
                     /fragment="True"
                     /other="protein_bind"
     CDS             1191..1283
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1587..1623)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
       61 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
      121 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
      181 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
      241 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
      301 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
      361 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     1201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1261 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     1321 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     1381 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     1441 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     1501 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     1561 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     1621 agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac taataccatc
     1681 taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg tagtctgttt
     1741 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
     1801 tttcttgtac aaagtggggc gcgccgacgt cccgcgggtc gaccctgcag gtacgtaact
     1861 agtctcgagc aactttatta tacaaagttg gcattataaa aaagcattgc ttatcaattt
     1921 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttggagc tccatggtag
     1981 cgttaacgcg gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2041 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2101 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     2161 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     2221 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     2281 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2341 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2401 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2461 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2521 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2581 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2641 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga a
//
