LOCUS       plasmid                 2682 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             617..1426
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1573..2161
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      complement(2491..2518)
                     /note="pLannotate"
                     /label="rrnB T2 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    complement(332..352)
                     /note="pLannotate"
                     /label="attB3"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(465..483)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(156..279)
                     /note="pLannotate"
                     /label="attR2"
                     /database="snapgene"
                     /identity="98.4"
                     /match_length="99.2"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(343..428)
                     /note="pLannotate"
                     /label="attL1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="86.0"
                     /fragment="True"
                     /other="protein_bind"
     terminator      complement(2610..2682)
                     /note="pLannotate"
                     /label="rrnB T1 terminator (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="89.0"
                     /fragment="True"
                     /other="terminator"
     CDS             2344..2436
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(49..85)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="21.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
       61 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat
      121 tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata tgttgtgttt
      181 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      241 cattttacgt ttctcgttca actttcttgt acaaagtggg gcgcgccgac gtcccgcggg
      301 tcgaccctgc aggtacgtaa ctagtctcga gcaactttat tatacaaagt tggcattata
      361 aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      421 attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta
      481 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      541 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      601 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
      661 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
      721 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
      781 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
      841 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
      901 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
      961 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1021 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1081 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1141 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1201 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1261 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1321 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1381 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     1441 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     1501 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     1561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1681 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1801 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1861 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1921 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1981 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2041 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2101 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2161 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2221 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2281 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2341 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2401 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     2461 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     2521 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     2581 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     2641 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cc
//
