LOCUS       plasmid                 8206 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    complement(5114..5702)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     intron          complement(7245..7720)
                     /note="pLannotate"
                     /label="β-globin intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     enhancer        complement(join(8058..8206,1..155))
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(3213..3437)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(3451..4242)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(4613..4749)
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(569..705)
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     polyA_signal    4940..5061
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(1579..1683)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(718..1578)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(4497..4568)
                     /note="pLannotate"
                     /label="3' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(353..424)
                     /note="pLannotate"
                     /label="5' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(5769..7127)
                     /note="pLannotate"
                     /label="IGG1_HUMAN"
                     /database="swissprot"
                     /identity="88.1"
                     /match_length="100.9"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2429..3017
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(7854..7905)
                     /note="pLannotate"
                     /label="CMVd2 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    236..284
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4256..4452)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      302..348
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(4779..4825)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     CDS             2193..2257
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="CDS"
     CDS             4848..4917
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="CDS"
     promoter        7968..7985
                     /note="pLannotate"
                     /label="CMV promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
       61 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
      121 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
      181 ctaatgaccc cgtaattgat tactagatct ggctacactc gatcagcagt tagataataa
      241 aatcgctatc catcgaagat ggatgtgtgt tggttttttg tgtgtgtaac gcaacgattg
      301 atagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgaa ttgagaggta
      361 gctgaagctg ctgttatgac cactagaggg aagaagatac ctgtggagct aatggtccaa
      421 gatgtcggga gccgcgatcg ctgccaaaaa ggtaagctga cgatagctga cgagattcga
      481 tgcccgacga gcaaatgagc gccacggctg ttcgcgtaag ctcgaaacga cggagattcg
      541 atagagccag agtcgagttt aaacgatctt aaggataaat ttctggtaag gaggacacgt
      601 atggaagtgg gcaagttggg gaagccgtat ccgttgctga atctggcata tgtgggagta
      661 taagacgcgc agcgtcgcat caggcatttt tttctgcgcc aatgcaccta gaatcggtta
      721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      961 tatcaactgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tacgcaaggt
     1021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1141 tagctccttc ggtcctccaa tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1261 gactggtgaa tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1381 cattggaaag cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1441 ttcgatgtaa cccactcgcg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1681 gcggcgaaag cctcctgctg atcctgtctc ttgatagatg tttgcaaaag ctaggactcg
     1741 attatacgtt gaccgatcag actatcaata tctaggttta aacagatcgg tacgctgccg
     1801 tgaccaggct tgagtacaat atctgacgat tggacttcct gggtcccaag ctggattccg
     1861 actggctgac tgccaagcaa cgatatggat cgtcgagact cgcactgaga ggatcgttct
     1921 cggtacacca ggcgcgcccc gatttcgata ttcctcggat ctcggataga tcgggattga
     1981 ggatgtaggt tgaacttctc tcgggtgatc ccgatctcgg atattcggga ttggagtaat
     2041 acctggcgat aggcgctgcc aagcaatagc caaggcgcaa ggccggccaa gagacttgcg
     2101 atagttcgtt cgagaggtag cctgcaagat ggatcggacg aaaggtttga agattttccg
     2161 acgagggcct tggagagttc ggaaatttaa atgcaactat ggatgaacga aatagacaga
     2221 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3061 ggccttttgc tcacatgatt taaatgaaac tcgtaatcgg agaacgggtg caatagcaag
     3121 gaccagatcg aacttgactg acgcaattct gagcgtagtc gaaacccaga caagtcgatc
     3181 tcgccaagta gctgacttga ctggttaatt aaccatagag cccaccgcat ccccagcatg
     3241 cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat
     3301 agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt
     3361 gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg
     3421 gcaactagaa ggcacagggc ttacatacta gaagaactcg tcaagaaggc gatagaaggc
     3481 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     3541 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     3601 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     3661 cggcaagcag gcatcgccgt gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt
     3721 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     3781 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     3841 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     3901 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     3961 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     4021 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tggagttcat tcagggcacc
     4081 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     4141 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     4201 agcggcggga gaacctgcgt gcaatccatc ttgttcaatc atgttggcta cctcctttgc
     4261 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     4321 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg atggggcgga gaatgggcgg
     4381 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     4441 aattgagatg cagatcgctt aattaagttt cgatagccca aggtaaccaa tgagattagg
     4501 gagggaagaa agtttgatga actacttctg acccctagtg gtgtccagaa aagaccatta
     4561 aaggaatgtc ttgatcgaaa tcgagagatc gcctgcagga tagctgacga ccttaaggat
     4621 aaatttctgg taaggaggac acgtatggaa gtgggcaagt tggggaagcc gtatccgttg
     4681 ctgaatctgg catatgtggg agtataagac gcgcagcgtc gcatcaggca tttttttctg
     4741 cgccaatgcc ggtagatagc aatccggccc gaggggcaca aaaaacccct caagacccgt
     4801 ttagaggccc caaggggtta tgctacgata ccgatagaga tgggccaata ccacatttgt
     4861 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4921 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4981 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5041 caaactcatc aatgtatctt atcatgtctg tgagctgaag gtacgctgta tctcagtcag
     5101 tcaagctaga caggcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg
     5161 cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga
     5221 ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag
     5281 gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc
     5341 aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca
     5401 atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa
     5461 ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc
     5521 cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc
     5581 atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa
     5641 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
     5701 ttaggcctgc ggatccagaa atcgattgta tcagtcagtc agtgcaggag gagacaactt
     5761 ctagactacc cgggggataa actcagtgac ttctgggtgt agtgattatg gagggcctcg
     5821 tgcattacac tgcaagagaa cacattgcct tgttgccagc ggctcttgtc aacggtcagc
     5881 ttggagtaca ggaaaaagct tccatcggag tcgagtacgg ggggtgtagt cttgtaatta
     5941 ttctcgggct gtccgttact ctcccattcg acggcaatat ccgatgggta aaagcccttc
     6001 acgagacatg tcagggaaac ctgattctta gtcatctctt ccctgctggg gggcagagtg
     6061 taaacctgag gctcgcgtgg ttgccctttt gccttagaga tggttttctc aatgggcgcg
     6121 ggtagtgcct tattggagac tttgcattta tattccttgc cgttgagcca gtcctggtgg
     6181 agcacagtca gcacagacac aacacggtag gtgctgttgt actgctcttc cctgggttta
     6241 gtctttgcgt tatgtacctc gactccatcg acataccagt tgaacttcac ctctgggtct
     6301 tcatgagaca cgtccaccac cacgcaagtt acctctgggg tccgggagat catgagtgtg
     6361 tcttttggtt taggaggaaa cagaaagacg gatggccccc ctagcagctc aggagctggg
     6421 catggggggc atgtgtgtgt cttatcgcaa gatttaggct caaccttctt atccactttt
     6481 gtgtttgagg gtttgtggtt tacgttgcaa atgtaagtct gcgtaccgag ggaactggaa
     6541 ggcactgtca ccacgctaga cagagaatac aggccagaac tctgcagcac agctgggaat
     6601 gtgtgcactc cagacgttag cgcgccactg ttccagctca ctgtaacagg ttcagggaag
     6661 tagtctttga cgagacatcc tagagcagcg gtgccgccgc ttgtagactt tgatgaagga
     6721 gccaatggga aaacagaggg gcccttggtg gaagctgagg agacggtgac cagggttccc
     6781 tggccccagg ggtcgaacca gtccaatttg tagtcaccgt ttacctctgt tgcacaataa
     6841 tagacggctg tgtcctcggt tttcaggctg ttcatttgca gataggcgat gccattggaa
     6901 tcatctctgg agatggtgaa tctgtctttc acagacgcgg cgcactcttt tgtcgcagca
     6961 taaatttcac ttcgaattag acacacccac tccagcccct tccctggagc ctggcggaac
     7021 caactaagag acctttcgcc tagactgatt ccagatcctg tacaggagag tctcagggac
     7081 cgccctggct tcaccaagcc tcccccagac tccaccagct gcacctcggc cagggcgcgc
     7141 ccagccaggc acagcaggaa aaagatccag gctctcatgg tggcggcgcg gccgcctttg
     7201 ccaaagtgat gggccagcac acagaccagc acgttgccca ggagctgtgg gaggaagata
     7261 agaggtatga acatgattag caaaagggcc tagcttggac tcagaataat ccagccttat
     7321 cccaaccata aaataaaagc agaatggtag ctggattgta gctgctatta gcaatatgaa
     7381 acctcttaca tcagttacaa tttatatgca gaaatattta tatgcagaaa tattgctatt
     7441 gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagagg catgatacat
     7501 tgtatcatta ttgccctgaa agaaagagat tagggaaagt attagaaata agataaacaa
     7561 aaaagtatat taaaagaaga aagcattttt taaaattaca aatgcaaaat taccctgatt
     7621 tggtcaatat gtgtaccctg ttacttctcc ccttcctatg acatgaactt aaccatagaa
     7681 aagaagggga aagaaaacat caagggtccc atagactcac cctgaagttc tcaggatccg
     7741 agctcggtac cacatgtaag cttcgagggg aggctggatc ggtcccggtg tcttctatgg
     7801 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa accagctctg
     7861 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     7921 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     7981 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     8041 tgccaaaacc gcatcagcat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     8101 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     8161 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactg
//
