LOCUS       plasmid                 8206 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    4248..4836
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     intron          2230..2705
                     /note="pLannotate"
                     /label="β-globin intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     enhancer        1589..1892
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    6513..6737
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             5708..6499
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      1039..1175
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      5201..5337
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     polyA_signal    complement(4889..5010)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        61..165
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             166..1026
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    5382..5453
                     /note="pLannotate"
                     /label="3' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    1320..1391
                     /note="pLannotate"
                     /label="5' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             2823..4181
                     /note="pLannotate"
                     /label="IGG1_HUMAN"
                     /database="swissprot"
                     /identity="87.7"
                     /match_length="100.9"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(6933..7521)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        2045..2096
                     /note="pLannotate"
                     /label="CMVd2 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1460..1508)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5498..5694
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      5125..5171
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(1396..1442)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(7693..7757)
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5033..5102)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(1965..1982)
                     /note="pLannotate"
                     /label="CMV promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 aatcgagtcc tagcttttgc aaacatctat caagagacag gatcagcagg aggctttcgc
       61 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      121 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      181 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      241 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcgcgagt gggttacatc
      301 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgctttcca
      361 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      421 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtattcacca
      481 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      541 accatgagtg ataacactgc ggccaactta cttctgacaa cgattggagg accgaaggag
      601 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      661 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      721 acaaccttgc gtaaactatt aactggcgaa ctacttactc tagcttcccg gcaacagttg
      781 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      841 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      901 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      961 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1021 tggtaaccga ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc
     1081 ttatactccc acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc
     1141 catacgtgtc ctccttacca gaaatttatc cttaagatcg tttaaactcg actctggctc
     1201 tatcgaatct ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg
     1261 gcatcgaatc tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac
     1321 atcttggacc attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc
     1381 agctacctct caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     1441 tatcaatcgt tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg
     1501 attttattat ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat
     1561 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     1621 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     1681 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1741 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1801 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1861 acatctacgt attagtcatc gctattacca tgctgatgcg gttttggcag tacatcaatg
     1921 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1981 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2041 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     2101 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     2161 accgggaccg atccagcctc ccctcgaagc ttacatgtgg taccgagctc ggatcctgag
     2221 aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta
     2281 agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa
     2341 ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt
     2401 atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc
     2461 ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat
     2521 ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct
     2581 aatagcagct acaatccagc taccattctg cttttatttt atggttggga taaggctgga
     2641 ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc
     2701 cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaaggcggcc
     2761 gcgccgccac catgagagcc tggatctttt tcctgctgtg cctggctggg cgcgccctgg
     2821 ccgaggtgca gctggtggag tctgggggag gcttggtgaa gccagggcgg tccctgagac
     2881 tctcctgtac aggatctgga atcagtcttg ccgaaaggtc tctgagttgg ttccgccagg
     2941 ctcctgggaa ggggctggag tgggtgtgtc taattcgaag tggaatttat gctgcgacag
     3001 aagaatgtgc cgcgtctgtg agagacagat tcattatctc aagagatgat tccaatggca
     3061 tcgcctatct gcaaatgaac agcctgaaag ccgaggatac agccgtctat tattgtgcag
     3121 cagaggtaag cggtgactac aaattggact ggttcgaccc ctggggccag ggaaccctgg
     3181 tcactgtctc ctcagcttcc accaagggcc cctctgtttt cccattggct ccttcatcaa
     3241 agtctacaag cggcggcacc gctgctctag gatgtctcgt caaagactac ttccctgaac
     3301 ctgttacagt gagctggaac agtggcgcgc taacgtctgg agtgcacaca ttcccagctg
     3361 tgctgcagag ttctggcctg tattctctgt ctagcgtggt gacagtgcct tccagttccc
     3421 tcggtacgca gacttacatt tgcaacgtaa accacaaacc ctcaaacaca aaagtggata
     3481 agaaggttga gcctaaatct tgcgataaga cacacacatg ccccccatgc ccagctcctg
     3541 agctgctagg ggggccatcc gtctttctgt ttcctcctaa accaaaagac acactcatga
     3601 tctcccggac cccagaggta acttgcgtgg tggtggacgt gtctcatgaa gacccagagg
     3661 tgaagttcaa ctggtatgtc gatggagtcg aggtacataa cgcaaagact aaacccaggg
     3721 aagagcagta caacagcacc taccgtgttg tgtctgtgct gactgtgctc caccaggact
     3781 ggctcaacgg caaggaatat aaatgcaaag tctccaataa ggcactaccc gcgcccattg
     3841 agaaaaccat ctctaaggca aaagggcaac cacgcgagcc tcaggtttac actctgcccc
     3901 ccagcaggga agagatgact aagaatcagg tttccctgac atgtctcgtg aagggctttt
     3961 acccatcgga tattgccgtc gaatgggaga gtaacggaca gcccgagaat aattacaaga
     4021 ctacaccccc cgtactcgac tccgatggaa gctttttcct gtactccaag ctgaccgttg
     4081 acaagagccg ctggcaacaa ggcaatgtgt tctcttgcag tgtaatgcac gaggccctcc
     4141 ataatcacta cacccagaag tcactgagtt tatcccccgg gtagtctaga agttgtctcc
     4201 tcctgcactg actgactgat acaatcgatt tctggatccg caggcctaat caacctctgg
     4261 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4321 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4381 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4441 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4501 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4561 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4621 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     4681 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4741 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4801 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ctagcttgac tgactgagat
     4861 acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca
     4921 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     4981 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     5041 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     5101 attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga
     5161 ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat
     5221 gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg
     5281 gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt
     5341 cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt
     5401 tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat
     5461 tggttacctt gggctatcga aacttaatta agcgatctgc atctcaatta gtcagcaacc
     5521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5581 ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaggaggt
     5701 agccaacatg attgaacaag atggattgca cgcaggttct cccgccgctt gggtggagag
     5761 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5821 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5881 tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5941 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6001 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6061 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6121 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6181 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat
     6241 gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt
     6301 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6361 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6421 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6481 ccttcttgac gagttcttct agtatgtaag ccctgtgcct tctagttgcc agccatctgt
     6541 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6601 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6661 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6721 tgcggtgggc tctatggtta attaaccagt caagtcagct acttggcgag atcgacttgt
     6781 ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc ttgctattgc
     6841 acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca gcaaaaggcc
     6901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7741 cgttcatcca tagttgcatt taaatttccg aactctccaa ggccctcgtc ggaaaatctt
     7801 caaacctttc gtccgatcca tcttgcaggc tacctctcga acgaactatc gcaagtctct
     7861 tggccggcct tgcgccttgg ctattgcttg gcagcgccta tcgccaggta ttactccaat
     7921 cccgaatatc cgagatcggg atcacccgag agaagttcaa cctacatcct caatcccgat
     7981 ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct ggtgtaccga gaacgatcct
     8041 ctcagtgcga gtctcgacga tccatatcgt tgcttggcag tcagccagtc ggaatccagc
     8101 ttgggaccca ggaagtccaa tcgtcagata ttgtactcaa gcctggtcac ggcagcgtac
     8161 cgatctgttt aaacctagat attgatagtc tgatcggtca acgtat
//
