LOCUS       plasmid                 8194 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    5356..5944
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     intron          3350..3825
                     /note="pLannotate"
                     /label="β-globin intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     enhancer        2709..3012
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    7621..7845
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             6816..7607
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      2159..2295
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      6309..6445
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     polyA_signal    complement(5997..6118)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        1181..1285
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1286..2146
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    6490..6561
                     /note="pLannotate"
                     /label="3' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    2440..2511
                     /note="pLannotate"
                     /label="5' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(join(8041..8194,1..435))
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3943..5289
                     /note="pLannotate"
                     /label="IGG1_HUMAN"
                     /database="swissprot"
                     /identity="84.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        3165..3216
                     /note="pLannotate"
                     /label="CMVd2 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(2580..2628)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6606..6802
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      complement(2516..2562)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     terminator      6233..6279
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(607..671)
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6141..6210)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(3085..3102)
                     /note="pLannotate"
                     /label="CMV promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
       61 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      661 tccatagttg catttaaatt tccgaactct ccaaggccct cgtcggaaaa tcttcaaacc
      721 tttcgtccga tccatcttgc aggctacctc tcgaacgaac tatcgcaagt ctcttggccg
      781 gccttgcgcc ttggctattg cttggcagcg cctatcgcca ggtattactc caatcccgaa
      841 tatccgagat cgggatcacc cgagagaagt tcaacctaca tcctcaatcc cgatctatcc
      901 gagatccgag gaatatcgaa atcggggcgc gcctggtgta ccgagaacga tcctctcagt
      961 gcgagtctcg acgatccata tcgttgcttg gcagtcagcc agtcggaatc cagcttggga
     1021 cccaggaagt ccaatcgtca gatattgtac tcaagcctgg tcacggcagc gtaccgatct
     1081 gtttaaacct agatattgat agtctgatcg gtcaacgtat aatcgagtcc tagcttttgc
     1141 aaacatctat caagagacag gatcagcagg aggctttcgc cgcggaaccc ctatttgttt
     1201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1261 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1321 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1381 agatgctgaa gatcagttgg gtgcgcgagt gggttacatc gaactggatc tcaacagcgg
     1441 taagatcctt gagagttttc gccccgaaga acgctttcca atgatgagca cttttaaagt
     1501 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1561 catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac
     1621 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1681 ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa
     1741 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1801 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaaccttgc gtaaactatt
     1861 aactggcgaa ctacttactc tagcttcccg gcaacagttg atagactgga tggaggcgga
     1921 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1981 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2041 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2101 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaaccga ttctaggtgc
     2161 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
     2221 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
     2281 gaaatttatc cttaagatcg tttaaactcg actctggctc tatcgaatct ccgtcgtttc
     2341 gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc tcgtcagcta
     2401 tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc attagctcca
     2461 caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct caattcaaaa
     2521 aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt tgcgttacac
     2581 acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat ctaactgctg
     2641 atcgagtgta gccagatcta gtaatcaatt acggggtcat tagttcatag cccatatatg
     2701 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2761 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2821 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2881 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2941 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3001 gctattacca tgctgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     3061 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     3121 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3181 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatcgcc
     3241 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     3301 ccctcgaagc ttacatgtgg taccgagctc ggatcctgag aacttcaggg tgagtctatg
     3361 ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg
     3421 gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt gtaattttaa
     3481 aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata ctttccctaa
     3541 tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac cattctaaag
     3601 aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat aaatatttct
     3661 gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct acaatccagc
     3721 taccattctg cttttatttt atggttggga taaggctgga ttattctgag tccaagctag
     3781 gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg
     3841 ctggtctgtg tgctggccca tcactttggc aaaggcggcc gcgccgccac catgagagcc
     3901 tggatctttt tcctgctgtg cctggctggg cgcgccctgg cccaggtgca gctgcaggag
     3961 tcgggcccag gactggtgaa gccttcggag accctgtccc tcacctgcag tgtctctggt
     4021 gcctccattc gtgatcacta cttgaactgg atccggcagc ccccaggggg gggactggag
     4081 tggattgggt atatctctta cagtgggtac accaactaca agccctccct caggaatcga
     4141 gtcaccataa cagcagacgc gtccaagaaa cagttctccc tgaagttgag ctctgtgacc
     4201 gctgcggaca cggccgtgta tttctgtgcg agagatggcg ggccgtatag tagggaaggt
     4261 tttgatatct ggggccaagg gacaatggtc accgtgtctt cagcttccac caagggcccc
     4321 tctgttttcc cattggctcc ttcatcaaag tctacaagcg gcggcaccgc tgctctagga
     4381 tgtctcgtca aagactactt ccctgaacct gttacagtga gctggaacag tggcgcgcta
     4441 acgtctggag tgcacacatt cccagctgtg ctgcagagtt ctggcctgta ttctctgtct
     4501 agcgtggtga cagtgccttc cagttccctc ggtacgcaga cttacatttg caacgtaaac
     4561 cacaaaccct caaacacaaa agtggataag aaggttgagc ctaaatcttg cgataagaca
     4621 cacacatgcc ccccatgccc agctcctgag ctgctagggg ggccatccgt ctttctgttt
     4681 cctcctaaac caaaagacac actcatgatc tcccggaccc cagaggtaac ttgcgtggtg
     4741 gtggacgtgt ctcatgaaga cccagaggtg aagttcaact ggtatgtcga tggagtcgag
     4801 gtacataacg caaagactaa acccagggaa gagcagtaca acagcaccta ccgtgttgtg
     4861 tctgtgctga ctgtgctcca ccaggactgg ctcaacggca aggaatataa atgcaaagtc
     4921 tccaataagg cactacccgc gcccattgag aaaaccatct ctaaggcaaa agggcaacca
     4981 cgcgagcctc aggtttacac tctgcccccc agcagggaag agatgactaa gaatcaggtt
     5041 tccctgacat gtctcgtgaa gggcttttac ccatcggata ttgccgtcga atgggagagt
     5101 aacggacagc ccgagaataa ttacaagact acaccccccg tactcgactc cgatggaagc
     5161 tttttcctgt actccaagct gaccgttgac aagagccgct ggcaacaagg caatgtgttc
     5221 tcttgcagtg taatgcacga ggccctccat aatcactaca cccagaagtc actgagttta
     5281 tcccccgggt agtctagaag ttgtctcctc ctgcactgac tgactgatac aatcgatttc
     5341 tggatccgca ggcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5401 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5461 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5521 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5581 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5641 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5701 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5761 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5821 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5881 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5941 ccgcctgtct agcttgactg actgagatac agcgtacctt cagctcacag acatgataag
     6001 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6061 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6121 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6181 aagcaagtaa aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa
     6241 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct
     6301 atctaccggc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc
     6361 acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc
     6421 ctccttacca gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt
     6481 cgatcaagac attcctttaa tggtcttttc tggacaccac taggggtcag aagtagttca
     6541 tcaaactttc ttccctccct aatctcattg gttaccttgg gctatcgaaa cttaattaag
     6601 cgatctgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6661 cctaactccg cccagttccg cccattctcc gccccatcgc tgactaattt tttttattta
     6721 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6781 tggaggccta ggcttttgca aaggaggtag ccaacatgat tgaacaagat ggattgcacg
     6841 caggttctcc cgccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     6901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6961 tcaagaccga cctgtccggt gccctgaatg aactccagga cgaggcagcg cggctatcgt
     7021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7321 aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc gtgacccacg
     7381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctag tatgtaagcc
     7621 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7681 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7741 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7801 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggttaat taaccagtca
     7861 agtcagctac ttggcgagat cgacttgtct gggtttcgac tacgctcaga attgcgtcag
     7921 tcaagttcga tctggtcctt gctattgcac ccgttctccg attacgagtt tcatttaaat
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atac
//
