LOCUS       plasmid                 7489 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    complement(5833..6421)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     enhancer        complement(571..874)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    complement(3932..4156)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(4170..4961)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(5332..5468)
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      complement(1288..1424)
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     polyA_signal    5659..5780
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(2298..2402)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1437..2297)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(6488..6805)
                     /note="pLannotate"
                     /label="IGLC2"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(5216..5287)
                     /note="pLannotate"
                     /label="3' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(1072..1143)
                     /note="pLannotate"
                     /label="5' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      3148..3736
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(367..418)
                     /note="pLannotate"
                     /label="CMVd2 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    955..1003
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     intron          complement(join(7253..7489,1..233))
                     /note="pLannotate"
                     /label="β-globin intron"
                     /database="snapgene"
                     /identity="98.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     promoter        complement(4975..5171)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(6842..7180)
                     /note="pLannotate"
                     /label="IGLV1-40"
                     /database="swissprot"
                     /identity="68.4"
                     /match_length="95.8"
                     /fragment="False"
                     /other="CDS"
     terminator      complement(5498..5544)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     terminator      1021..1067
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     CDS             2912..2976
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="CDS"
     CDS             5567..5636
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="CDS"
     promoter        481..498
                     /note="pLannotate"
                     /label="CMV promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 aggcatgata cattgtatca ttattgccct gaaagaaaga gattagggaa agtattagaa
       61 ataagataaa caaaaaagta tattaaaaga agaaagcatt ttttaaaatt acaaatgcaa
      121 aattaccctg atttggtcaa tatgtgtacc ctgttacttc tccccttcct atgacatgaa
      181 cttaaccata gaaaagaagg ggaaagaaaa catcaagggt cccatagact caccctgaag
      241 ttctcaggat ccgagctcgg taccacatgt aagcttcgag gggaggctgg atcggtcccg
      301 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
      361 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      421 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      481 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      541 cccattgatg tactgccaaa accgcatcag catggtaata gcgatgacta atacgtagat
      601 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      661 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      721 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      781 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      841 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      901 taatgacccc gtaattgatt actagatctg gctacactcg atcagcagtt agataataaa
      961 atcgctatcc atcgaagatg gatgtgtgtt ggttttttgt gtgtgtaacg caacgattga
     1021 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgaat tgagaggtag
     1081 ctgaagctgc tgttatgacc actagaggga agaagatacc tgtggagcta atggtccaag
     1141 atgtcgggag ccgcgatcgc tgccaaaaag gtaagctgac gatagctgac gagattcgat
     1201 gcccgacgag caaatgagcg ccacggctgt tcgcgtaagc tcgaaacgac ggagattcga
     1261 tagagccaga gtcgagttta aacgatctta aggataaatt tctggtaagg aggacacgta
     1321 tggaagtggg caagttgggg aagccgtatc cgttgctgaa tctggcatat gtgggagtat
     1381 aagacgcgca gcgtcgcatc aggcattttt ttctgcgcca atgcacctag aatcggttac
     1441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1681 atcaactgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt acgcaaggtt
     1741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1861 agctccttcg gtcctccaat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1981 actggtgaat actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2101 attggaaagc gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2161 tcgatgtaac ccactcgcgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2401 cggcgaaagc ctcctgctga tcctgtctct tgatagatgt ttgcaaaagc taggactcga
     2461 ttatacgttg accgatcaga ctatcaatat ctaggtttaa acagatcggt acgctgccgt
     2521 gaccaggctt gagtacaata tctgacgatt ggacttcctg ggtcccaagc tggattccga
     2581 ctggctgact gccaagcaac gatatggatc gtcgagactc gcactgagag gatcgttctc
     2641 ggtacaccag gcgcgccccg atttcgatat tcctcggatc tcggatagat cgggattgag
     2701 gatgtaggtt gaacttctct cgggtgatcc cgatctcgga tattcgggat tggagtaata
     2761 cctggcgata ggcgctgcca agcaatagcc aaggcgcaag gccggccaag agacttgcga
     2821 tagttcgttc gagaggtagc ctgcaagatg gatcggacga aaggtttgaa gattttccga
     2881 cgagggcctt ggagagttcg gaaatttaaa tgcaactatg gatgaacgaa atagacagat
     2941 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3001 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3061 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3121 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3181 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3241 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3301 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3361 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3421 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3481 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3541 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3601 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3661 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3721 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3781 gccttttgct cacatgattt aaatgaaact cgtaatcgga gaacgggtgc aatagcaagg
     3841 accagatcga acttgactga cgcaattctg agcgtagtcg aaacccagac aagtcgatct
     3901 cgccaagtag ctgacttgac tggttaatta accatagagc ccaccgcatc cccagcatgc
     3961 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     4021 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     4081 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     4141 caactagaag gcacagggct tacatactag aagaactcgt caagaaggcg atagaaggcg
     4201 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4261 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     4321 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4381 ggcaagcagg catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg
     4441 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4501 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     4561 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4621 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4681 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     4741 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct ggagttcatt cagggcaccg
     4801 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4861 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4921 gcggcgggag aacctgcgtg caatccatct tgttcaatca tgttggctac ctcctttgca
     4981 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     5041 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcga tggggcggag aatgggcgga
     5101 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     5161 attgagatgc agatcgctta attaagtttc gatagcccaa ggtaaccaat gagattaggg
     5221 agggaagaaa gtttgatgaa ctacttctga cccctagtgg tgtccagaaa agaccattaa
     5281 aggaatgtct tgatcgaaat cgagagatcg cctgcaggat agctgacgac cttaaggata
     5341 aatttctggt aaggaggaca cgtatggaag tgggcaagtt ggggaagccg tatccgttgc
     5401 tgaatctggc atatgtggga gtataagacg cgcagcgtcg catcaggcat ttttttctgc
     5461 gccaatgccg gtagatagca atccggcccg aggggcacaa aaaacccctc aagacccgtt
     5521 tagaggcccc aaggggttat gctacgatac cgatagagat gggccaatac cacatttgta
     5581 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     5641 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5761 aaactcatca atgtatctta tcatgtctgt gagctgaagg tacgctgtat ctcagtcagt
     5821 caagctagac aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc
     5881 gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat
     5941 tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg
     6001 cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca
     6061 acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa
     6121 tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac
     6181 cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc
     6241 acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca
     6301 tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     6361 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     6421 taggcctgcg gatccagaaa tcgattgtat cagtcagtca gtgcaggagg agacaacttc
     6481 tagactaact acactctgtt ggagccactg ttttctccac tgtggagccc tcatgtgtga
     6541 cctgacagct gtaggaccta tgagacttcc attgttccgg ggtcagggac agataggagc
     6601 tggctgcgta cttgttgttg ctttgcttgg agggtgtggt tgtttcaaca ccagccttca
     6661 caggcgaaga atctgccttc catgccacag tcacggcccc ggggtaaaaa tcggagataa
     6721 ggcacaccag ggtagcttta tttgcctgca attcttcgga ggatggggga aacagggtaa
     6781 cggatggggc tgccttgggc tggcctcgga cggtcagctt ggtccctccg ccaaacacgg
     6841 gaccatgcag gctgtcatcc caagctgcac agtagtaatc agcctcatcc tcggaccgga
     6901 gcccactgat ggccagggag gctgaggtgc cagacttgga gccagagaat cggtcaggga
     6961 ccgctgaggg ccgccgatta tccctataga tgaggagttt gggggccgtt cctgggaggt
     7021 gctgatacca agacacataa ttagttccga tgttgggcct agttccaaga caagagatgg
     7081 agacccgctg cccgggggtc ccagacgctg agggtggctg agtcagcaca gactgggcca
     7141 gggcgcgccc agccaggcac agcaggaaaa agatccaggc tctcatggtg gcggcgcggc
     7201 cgcctttgcc aaagtgatgg gccagcacac agaccagcac gttgcccagg agctgtggga
     7261 ggaagataag aggtatgaac atgattagca aaagggccta gcttggactc agaataatcc
     7321 agccttatcc caaccataaa ataaaagcag aatggtagct ggattgtagc tgctattagc
     7381 aatatgaaac ctcttacatc agttacaatt tatatgcaga aatatttata tgcagaaata
     7441 ttgctattgc cttaacccag aaattatcac tgttattctt tagaatggt
//
