LOCUS       plasmid                 8344 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    366..954
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    7755..8343
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     intron          6448..6923
                     /note="pLannotate"
                     /label="β-globin intron"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     enhancer        5807..6110
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     polyA_signal    2525..2749
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             1720..2511
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      1319..1455
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      5257..5393
                     /note="pLannotate"
                     /label="rrnG terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     polyA_signal    complement(1007..1128)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4279..4383
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             4384..5244
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7371..7688
                     /note="pLannotate"
                     /label="IGLC2"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    5538..5609
                     /note="pLannotate"
                     /label="5' β-globin insulator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(2945..3533)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        6263..6314
                     /note="pLannotate"
                     /label="CMVd2 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(5678..5726)
                     /note="pLannotate"
                     /label="poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             6996..7331
                     /note="pLannotate"
                     /label="IGLV1-50 (fragment)"
                     /database="swissprot"
                     /identity="72.4"
                     /match_length="94.9"
                     /fragment="True"
                     /other="CDS"
     terminator      complement(5614..5660)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     terminator      1243..1289
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="97.9"
                     /fragment="False"
                     /other="terminator"
     CDS             complement(3705..3769)
                     /note="pLannotate"
                     /label="bla(M) (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1151..1220)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(6183..6200)
                     /note="pLannotate"
                     /label="CMV promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="9.0"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 tgtccgttac cctgtttccc ccatcctccg aagaattgca ggcaaataaa gctaccctgg
       61 tgtgccttat ctccgatttt taccccgggg ccgtgactgt ggcatggaag gcagattctt
      121 cgcctgtgaa ggctggtgtt gaaacaacca caccctccaa gcaaagcaac aacaagtacg
      181 cagccagctc ctatctgtcc ctgaccccgg aacaatggaa gtctcatagg tcctacagct
      241 gtcaggtcac acatgagggc tccacagtgg agaaaacagt ggctccaaca gagtgtagtt
      301 agtctagaag ttgtctcctc ctgcactgac tgactgatac aatcgatttc tggatccgca
      361 ggcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
      421 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
      481 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
      541 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
      601 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
      661 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
      721 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
      781 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
      841 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
      901 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtct
      961 agcttgactg actgagatac agcgtacctt cagctcacag acatgataag atacattgat
     1021 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1081 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     1141 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     1201 aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa ccccttgggg
     1261 cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct atctaccggc
     1321 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
     1381 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
     1441 gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt cgatcaagac
     1501 attcctttaa tggtcttttc tggacaccag gggtcagaag tacacaaatt tttccctcca
     1561 atggattggg ctacgaaaaa aaagagaaat agtcagaaag cccccccccc cccgcccgtt
     1621 ccgccctccg ccccagattt tttaaatatg gagggcgagg gcgcccggcc cgaaaggggg
     1681 gggttttttg gggcgggttt tgcaaaggag gtagccaaca tgattgaaca agatggattg
     1741 cacgcaggtt ctcccgccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     1801 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     1861 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta
     1921 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     1981 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     2041 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     2101 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     2161 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     2221 gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc
     2281 cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     2341 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     2401 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     2461 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctagtatgta
     2521 agccctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2581 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2641 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2701 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt taattaacca
     2761 gtcaagtcag ctacttggcg agatcgactt gtctgggttt cgactacgct cagaattgcg
     2821 tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg agtttcattt
     2881 aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2941 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3001 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3061 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3121 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3181 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3241 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3301 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3361 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3421 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3481 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3541 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3601 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3661 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3721 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca tttaaatttc
     3781 cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag
     3841 gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt ggctattgct
     3901 tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg ggatcacccg
     3961 agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga atatcgaaat
     4021 cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac gatccatatc
     4081 gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc aatcgtcaga
     4141 tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag atattgatag
     4201 tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca agagacagga
     4261 tcagcaggag gctttcgccg cggaacccct atttgtttat ttttctaaat acattcaaat
     4321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4501 gcgcgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4561 cccgaagaac gctttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4681 ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4801 attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4921 atgcctgtag caatggcaac aaccttgcgt aaactattaa ctggcgaact acttactcta
     4981 gcttcccggc aacagttgat agactggatg gaggcggata aagttgcagg accacttctg
     5041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5221 gcctcactga ttaagcattg gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct
     5281 gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt
     5341 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagatcgtt
     5401 taaactcgac tctggctcta tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg
     5461 cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac ctttttggca
     5521 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt
     5581 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga
     5641 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc
     5701 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt
     5761 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     5821 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     5881 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5941 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6001 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6061 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt
     6121 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     6181 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     6241 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     6301 atataagcag agctggttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     6361 ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt acatgtggta
     6421 ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc
     6481 ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata
     6541 ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata
     6601 tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa
     6661 tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg
     6721 ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt
     6781 aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttat
     6841 ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca
     6901 tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc
     6961 actttggcaa aggcggccgc gccgccacca tgagagcctg gatctttttc ctgctgtgcc
     7021 tggctgggcg cgccctggcc cagtctgtgc tgactcagcc accctcagcg tctgggaccc
     7081 ccgggcagcg ggtctccatc tcttgtgttg gaactagctc caacatcgga actaattatg
     7141 tgtcttggta tcagcacctc ccacgaacgg cccccaaact cctcatctat aggaataatc
     7201 ggcggccctc agcggtccct gaccgattct ctggctccaa gtctggcacc tcagcctccc
     7261 tggccatcag tgggctccgg tccgaggatg aggctgatta ttactgtgca gcttgggatg
     7321 acagcctgaa tggtcccgtg tttggcggag ggaccaagct gaccgtccta ggccagccca
     7381 aggcagcccc atccgttacc ctgtttcccc catcctccga agaattgcag gcaaataaag
     7441 ctaccctggt gtgccttatc tccgattttt accccggggc cgtgactgtg gcatggaagg
     7501 cagattcttc gcctgtgaag gctggtgttg aaacaaccac accctccaag caaagcaaca
     7561 acaagtacgc agccagctcc tatctgtccc tgaccccgga acaatggaag tctcataggt
     7621 cctacagctg tcaggtcaca catgagggct ccacagtgga gaaaacagtg gctccaacag
     7681 agtgtagtta gtctagaagt tgtctcctcc tgcactgact gactgataca atcgatttct
     7741 ggatccgcag gcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     8041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     8101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     8161 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8341 cgcc
//
