LOCUS plasmid 8344 bp DNA circular SYN 22-JAN-2026 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE . ORGANISM . . COMMENT Annotated with pLannotate v1.2.0 FEATURES Location/Qualifiers misc_feature 366..954 /note="pLannotate" /label="WPRE" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="misc_feature" misc_feature 7755..8343 /note="pLannotate" /label="WPRE" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="misc_feature" intron 6448..6923 /note="pLannotate" /label="β-globin intron" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="intron" enhancer 5807..6110 /note="pLannotate" /label="CMV enhancer" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="enhancer" polyA_signal 2525..2749 /note="pLannotate" /label="bGH poly(A) signal" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="polyA_signal" CDS 1720..2511 /note="pLannotate" /label="neo" /database="swissprot" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" terminator 1319..1455 /note="pLannotate" /label="rrnG terminator" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="terminator" terminator 5257..5393 /note="pLannotate" /label="rrnG terminator" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="terminator" polyA_signal complement(1007..1128) /note="pLannotate" /label="SV40 poly(A) signal" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="polyA_signal" promoter 4279..4383 /note="pLannotate" /label="AmpR promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS 4384..5244 /note="pLannotate" /label="AmpR" /database="snapgene" /identity="98.8" /match_length="100.0" /fragment="False" /other="CDS" CDS 7371..7688 /note="pLannotate" /label="IGLC2" /database="swissprot" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" misc_feature 5538..5609 /note="pLannotate" /label="5' β-globin insulator" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="misc_feature" rep_origin complement(2945..3533) /note="pLannotate" /label="ori" /database="snapgene" /identity="99.8" /match_length="100.0" /fragment="False" /other="rep_origin" promoter 6263..6314 /note="pLannotate" /label="CMVd2 promoter" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" polyA_signal complement(5678..5726) /note="pLannotate" /label="poly(A) signal" /database="snapgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="polyA_signal" CDS 6996..7331 /note="pLannotate" /label="IGLV1-50 (fragment)" /database="swissprot" /identity="72.4" /match_length="94.9" /fragment="True" /other="CDS" terminator complement(5614..5660) /note="pLannotate" /label="T7 terminator" /database="snapgene" /identity="100.0" /match_length="97.9" /fragment="False" /other="terminator" terminator 1243..1289 /note="pLannotate" /label="T7 terminator" /database="snapgene" /identity="100.0" /match_length="97.9" /fragment="False" /other="terminator" CDS complement(3705..3769) /note="pLannotate" /label="bla(M) (fragment)" /database="snapgene" /identity="100.0" /match_length="8.2" /fragment="True" /other="CDS" CDS complement(1151..1220) /note="pLannotate" /label="large T antigen (fragment)" /database="snapgene" /identity="100.0" /match_length="3.3" /fragment="True" /other="CDS" promoter complement(6183..6200) /note="pLannotate" /label="CMV promoter (fragment)" /database="snapgene" /identity="100.0" /match_length="9.0" /fragment="True" /other="promoter" ORIGIN 1 tgtccgttac cctgtttccc ccatcctccg aagaattgca ggcaaataaa gctaccctgg 61 tgtgccttat ctccgatttt taccccgggg ccgtgactgt ggcatggaag gcagattctt 121 cgcctgtgaa ggctggtgtt gaaacaacca caccctccaa gcaaagcaac aacaagtacg 181 cagccagctc ctatctgtcc ctgaccccgg aacaatggaa gtctcatagg tcctacagct 241 gtcaggtcac acatgagggc tccacagtgg agaaaacagt ggctccaaca gagtgtagtt 301 agtctagaag ttgtctcctc ctgcactgac tgactgatac aatcgatttc tggatccgca 361 ggcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 421 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 481 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 541 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 601 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 661 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 721 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 781 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 841 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 901 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgtct 961 agcttgactg actgagatac agcgtacctt cagctcacag acatgataag atacattgat 1021 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt 1081 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat 1141 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa 1201 aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa ccccttgggg 1261 cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct atctaccggc 1321 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca 1381 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca 1441 gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt cgatcaagac 1501 attcctttaa tggtcttttc tggacaccag gggtcagaag tacacaaatt tttccctcca 1561 atggattggg ctacgaaaaa aaagagaaat agtcagaaag cccccccccc cccgcccgtt 1621 ccgccctccg ccccagattt tttaaatatg gagggcgagg gcgcccggcc cgaaaggggg 1681 gggttttttg gggcgggttt tgcaaaggag gtagccaaca tgattgaaca agatggattg 1741 cacgcaggtt ctcccgccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 1801 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 1861 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta 1921 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 1981 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 2041 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 2101 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 2161 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 2221 gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct cgtcgtgacc 2281 cacggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 2341 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 2401 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 2461 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctagtatgta 2521 agccctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 2581 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 2641 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 2701 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt taattaacca 2761 gtcaagtcag ctacttggcg agatcgactt gtctgggttt cgactacgct cagaattgcg 2821 tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg agtttcattt 2881 aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 2941 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3001 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3061 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 3121 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 3181 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 3241 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 3301 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 3361 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 3421 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 3481 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 3541 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 3601 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 3661 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 3721 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca tttaaatttc 3781 cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag 3841 gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt ggctattgct 3901 tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg ggatcacccg 3961 agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga atatcgaaat 4021 cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac gatccatatc 4081 gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc aatcgtcaga 4141 tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag atattgatag 4201 tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca agagacagga 4261 tcagcaggag gctttcgccg cggaacccct atttgtttat ttttctaaat acattcaaat 4321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 4381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 4441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 4501 gcgcgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 4561 cccgaagaac gctttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 4621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 4681 ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 4741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 4801 attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 4861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 4921 atgcctgtag caatggcaac aaccttgcgt aaactattaa ctggcgaact acttactcta 4981 gcttcccggc aacagttgat agactggatg gaggcggata aagttgcagg accacttctg 5041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 5101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 5161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 5221 gcctcactga ttaagcattg gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct 5281 gatgcgacgc tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggctt 5341 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagatcgtt 5401 taaactcgac tctggctcta tcgaatctcc gtcgtttcga gcttacgcga acagccgtgg 5461 cgctcatttg ctcgtcgggc atcgaatctc gtcagctatc gtcagcttac ctttttggca 5521 gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt 5581 ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga 5641 ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc 5701 catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctagt 5761 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta 5821 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga 5881 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt 5941 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta 6001 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg 6061 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg ctgatgcggt 6121 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc 6181 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat 6241 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct 6301 atataagcag agctggttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt 6361 ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt acatgtggta 6421 ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg ttttctttcc 6481 ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag ggtacacata 6541 ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct tcttttaata 6601 tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa 6661 tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg 6721 ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt 6781 aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttat 6841 ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca 6901 tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc 6961 actttggcaa aggcggccgc gccgccacca tgagagcctg gatctttttc ctgctgtgcc 7021 tggctgggcg cgccctggcc cagtctgtgc tgactcagcc accctcagcg tctgggaccc 7081 ccgggcagcg ggtctccatc tcttgtgttg gaactagctc caacatcgga actaattatg 7141 tgtcttggta tcagcacctc ccacgaacgg cccccaaact cctcatctat aggaataatc 7201 ggcggccctc agcggtccct gaccgattct ctggctccaa gtctggcacc tcagcctccc 7261 tggccatcag tgggctccgg tccgaggatg aggctgatta ttactgtgca gcttgggatg 7321 acagcctgaa tggtcccgtg tttggcggag ggaccaagct gaccgtccta ggccagccca 7381 aggcagcccc atccgttacc ctgtttcccc catcctccga agaattgcag gcaaataaag 7441 ctaccctggt gtgccttatc tccgattttt accccggggc cgtgactgtg gcatggaagg 7501 cagattcttc gcctgtgaag gctggtgttg aaacaaccac accctccaag caaagcaaca 7561 acaagtacgc agccagctcc tatctgtccc tgaccccgga acaatggaag tctcataggt 7621 cctacagctg tcaggtcaca catgagggct ccacagtgga gaaaacagtg gctccaacag 7681 agtgtagtta gtctagaagt tgtctcctcc tgcactgact gactgataca atcgatttct 7741 ggatccgcag gcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 7801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 7861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 7921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 7981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 8041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 8101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 8161 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 8221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 8281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 8341 cgcc //